Mouse Depdc5 activation kit by CRISPRa

CAT#: GA216165

Depdc5 CRISPRa kit - CRISPR gene activation of mouse DEP domain containing 5

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Depdc5
• Locus ID: 277854
• Kit Components:

  GA216165G1, Depdc5 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA216165G2, Depdc5 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA216165G3, Depdc5 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001025426, NM_001170567, NM_177786, NM_001360014
• UniProt ID: P61460
• Synonyms: AV016528
• Summary: As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.[UniProtKB/Swiss-Prot Function]