Mouse Drosha activation kit by CRISPRa

CAT#: GA201307

Drosha CRISPRa kit - CRISPR gene activation of mouse drosha, ribonuclease type III

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Drosha
• Locus ID: 14000
• Kit Components:

  GA201307G1, Drosha gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA201307G2, Drosha gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA201307G3, Drosha gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001130149, NM_026799
• UniProt ID: Q5HZJ0
• Synonyms: 1110013A17Rik; AI874853; Etohi2; Rn3; Rnasen
• Summary: Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.[UniProtKB/Swiss-Prot Function]