Human REST activation kit by CRISPRa

CAT#: GA104072

REST CRISPRa kit - CRISPR gene activation of human RE1 silencing transcription factor

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: REST
• Locus ID: 5978
• Kit Components:

  GA104072G1, REST gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104072G2, REST gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104072G3, REST gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001193508, NM_005612, NM_001363453
• UniProt ID: Q13127
• Synonyms: NRSF; WT6; XBR
• Summary: This gene was initially identified as a transcriptional repressor that represses neuronal genes in non-neuronal tissues. However, depending on the cellular context, this gene can act as either an oncogene or a tumor suppressor. The encoded protein is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regulator of neurogenesis. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2018]