Human Rb (RB1) activation kit by CRISPRa

CAT#: GA104035

RB1 CRISPRa kit - CRISPR gene activation of human RB transcriptional corepressor 1

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: RB1
• Locus ID: 5925
• Kit Components:

  GA104035G1, Rb gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA104035G2, Rb gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA104035G3, Rb gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000321
• UniProt ID: P06400
• Synonyms: OSRC; p105-Rb; pp110; PPP1R130; pRb; RB
• Summary: The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]