Human CBS activation kit by CRISPRa

CAT#: GA100617

CBS CRISPRa kit - CRISPR gene activation of human cystathionine-beta-synthase

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: CBS
• Locus ID: 875
• Kit Components:

  GA100617G1, CBS gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA100617G2, CBS gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA100617G3, CBS gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_000071, NM_001178008, NM_001178009, NM_001320298, NM_001321072
• UniProt ID: P35520
• Synonyms: HIP4
• Summary: The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. This gene is a major contributor to cellular hydrogen sulfide production. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2016]