Menin (MEN1) (NM_130799) Human Mutant ORF Clone

CAT#: RC403519

MEN1 Mutant (G42D), Myc-DDK-tagged ORF clone of Homo sapiens multiple endocrine neoplasia I (MEN1), transcript variant 2 as transfection-ready DNA

Specifications

Product Data

• Mutation Description: G42D
• Affected Codon#: 42
• Affected NT#: 125
• Tag: Myc-DDK
• Effect: Multiple endocrine neoplasia 1
• Symbol: MEN1
• Synonyms: MEAI; SCG2
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Vector: pCMV6-Entry
• ACCN: NM_130799
• ORF Size: 1830 bp
• Sequence Data:
  ORF Nucleotide Sequence

  >RC403519 representing NM_130799
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTGAAGGCCGCCCAGAAGACGCTGTTCCCGCTGCGCTCCATCGACGACGTGGTGCGCCTGTTTG
  CTGCCGAGCTGGGCCGAGAGGAGCCGGACCTGGTGCTCCTTTCCTTGGTGCTGGACTTCGTGGAGCATTT
  TCTGGCTGTCAACCGCGTCATCCCTACCAACGTTCCCGAGCTCACCTTCCAGCCCAGCCCCGCCCCCGAC
  CCGCCTGGCGGCCTCACCTACTTTCCCGTGGCCGACCTGTCTATCATCGCCGCCCTCTATGCCCGCTTCA
  CCGCCCAGATCCGAGGCGCCGTCGACCTGTCCCTCTATCCTCGAGAAGGGGGTGTCTCCAGCCGTGAGCT
  GGTGAAGAAGGTCTCCGATGTCATATGGAACAGCCTCAGCCGCTCCTACTTCAAGGATCGGGCCCACATC
  CAGTCCCTCTTCAGCTTCATCACAGGCACCAAATTGGACAGCTCCGGTGTGGCCTTTGCTGTGGTTGGGG
  CCTGCCAGGCCCTGGGTCTCCGGGATGTCCACCTCGCCCTGTCTGAGGATCATGCCTGGGTAGTGTTTGG
  GCCCAATGGGGAGCAGACAGCTGAGGTCACCTGGCACGGCAAGGGCAACGAGGACCGCAGGGGCCAGACA
  GTCAATGCCGGTGTGGCTGAGCGGAGCTGGCTGTACCTGAAAGGATCATACATGCGCTGTGACCGCAAGA
  TGGAGGTGGCGTTCATGGTGTGTGCCATCAACCCTTCCATTGACCTGCACACCGACTCGCTGGAGCTTCT
  GCAGCTGCAGCAGAAGCTGCTCTGGCTGCTCTATGACCTGGGACATCTGGAAAGGTACCCCATGGCCTTA
  GGGAACCTGGCAGATCTAGAGGAGCTGGAGCCCACCCCTGGCCGGCCAGACCCACTCACCCTCTACCACA
  AGGGCATTGCCTCAGCCAAGACCTACTATCGGGATGAACACATCTACCCCTACATGTACCTGGCTGGCTA
  CCACTGTCGCAACCGCAATGTGCGGGAAGCCCTGCAGGCCTGGGCGGACACGGCCACTGTCATCCAGGAC
  TACAACTACTGCCGGGAAGACGAGGAGATCTACAAGGAGTTCTTTGAAGTAGCCAATGATGTCATCCCCA
  ACCTGCTGAAGGAGGCAGCCAGCTTGCTGGAGGCGGGCGAGGAGCGGCCGGGGGAGCAAAGCCAGGGCAC
  CCAGAGCCAAGGTTCCGCCCTCCAGGACCCTGAGTGCTTCGCCCACCTGCTGCGATTCTACGACGGCATC
  TGCAAATGGGAGGAGGGCAGTCCCACGCCTGTGCTGCACGTGGGCTGGGCCACCTTTCTTGTGCAGTCCC
  TAGGCCGTTTTGAGGGACAGGTGCGGCAGAAGGTGCGCATAGTGAGCCGAGAGGCCGAGGCGGCCGAGGC
  CGAGGAGCCGTGGGGCGAGGAAGCCCGGGAAGGCCGGCGGCGGGGCCCACGGCGGGAGTCCAAGCCAGAG
  GAGCCCCCGCCGCCCAAGAAGCCAGCACTGGACAAGGGCCTGGGCACCGGCCAGGGTGCAGTGTCAGGAC
  CCCCCCGGAAGCCTCCTGGGACTGTCGCTGGCACAGCCCGAGGCCCTGAAGGTGGCAGCACGGCTCAGGT
  GCCAGCACCCGCAGCATCACCACCGCCGGAGGGTCCAGTGCTCACTTTCCAGAGTGAGAAGATGAAGGGC
  ATGAAGGAGCTGCTGGTGGCCACCAAGATCAACTCGAGCGCCATCAAGCTGCAACTCACGGCACAGTCGC
  AAGTGCAGATGAAGAAGCAGAAAGTGTCCACCCCTAGTGACTACACTCTGTCTTTCCTCAAGCGGCAGCG
  CAAAGGCCTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGA TAAGGTTTAA
  Protein Sequence

  >RC403519 representing NM_130799
  Red=Cloning site Green=Tags(s)
  
  MGLKAAQKTLFPLRSIDDVVRLFAAELGREEPDLVLLSLVLDFVEHFLAVNRVIPTNVPELTFQPSPAPD
  PPGGLTYFPVADLSIIAALYARFTAQIRGAVDLSLYPREGGVSSRELVKKVSDVIWNSLSRSYFKDRAHI
  QSLFSFITGTKLDSSGVAFAVVGACQALGLRDVHLALSEDHAWVVFGPNGEQTAEVTWHGKGNEDRRGQT
  VNAGVAERSWLYLKGSYMRCDRKMEVAFMVCAINPSIDLHTDSLELLQLQQKLLWLLYDLGHLERYPMAL
  GNLADLEELEPTPGRPDPLTLYHKGIASAKTYYRDEHIYPYMYLAGYHCRNRNVREALQAWADTATVIQD
  YNYCREDEEIYKEFFEVANDVIPNLLKEAASLLEAGEERPGEQSQGTQSQGSALQDPECFAHLLRFYDGI
  CKWEEGSPTPVLHVGWATFLVQSLGRFEGQVRQKVRIVSREAEAAEAEEPWGEEAREGRRRGPRRESKPE
  EPPPPKKPALDKGLGTGQGAVSGPPRKPPGTVAGTARGPEGGSTAQVPAPAASPPPEGPVLTFQSEKMKG
  MKELLVATKINSSAIKLQLTAQSQVQMKKQKVSTPSDYTLSFLKRQRKGL
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NP_570711
• RefSeq Size: 1830 bp
• RefSeq ORF: 1833 bp
• Locus ID: 4221
• Cytogenetics: 11q13.1
• Domains: Menin
• Protein Families: Druggable Genome, Transcription Factors
• MW: 67.1 kDa
• Gene Summary: This gene encodes menin, a tumor suppressor associated with a syndrome known as multiple endocrine neoplasia type 1. Menin is a scaffold protein that functions in histone modification and epigenetic gene regulation. It is thought to regulate several pathways and processes by altering chromatin structure through the modification of histones. [provided by RefSeq, May 2019]