Bap1 (NM_001107292) Rat Tagged ORF Clone Lentiviral Particle

CAT#: RR205856L4V

Lenti ORF particles, Bap1 (GFP-tagged ORF) - Rat BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) (Bap1), 200ul, >10^7 TU/mL

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: mGFP
• Symbol: Bap1
• Mammalian Cell Selection: Puromycin
• Vector: pLenti-C-mGFP-P2A-Puro
• ACCN: NM_001107292
• ORF Size: 2220 bp
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RR205856).
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001107292.1, NP_001100762.1
• RefSeq Size: 3751 bp
• RefSeq ORF: 2223 bp
• Locus ID: 306257
• UniProt ID: D3ZHS6
• Cytogenetics: 16p16
• Gene Summary: Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration. Acts as a tumor suppressor.[UniProtKB/Swiss-Prot Function]