SPTLC1 (NM_178324) Human Tagged ORF Clone Lentiviral Particle

CAT#: RC216310L4V

Lenti ORF particles, SPTLC1 (mGFP-tagged)-Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), transcript variant 2, 200ul, >10^7 TU/mL

Product Images

RC216310L4 was used to prepare Lentiviral particles using TR30037 packaging kit. HEK293T cells were transduced with RC216310L4V particle to overexpress human SPTLC1-mGFP fusion protein.

Specifications

Product Data

• Type: Human Tagged ORF Clone Lentiviral Particle
• Tag: mGFP
• Symbol: SPTLC1
• Synonyms: HSAN1; HSN1; LBC1; LCB1; SPT1; SPTI
• Mammalian Cell Selection: Puromycin
• Vector: pLenti-C-mGFP-P2A-Puro
• ACCN: NM_178324
• ORF Size: 429 bp
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC216310).
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_178324.1
• RefSeq Size: 998 bp
• RefSeq ORF: 432 bp
• Locus ID: 10558
• UniProt ID: O15269
• Cytogenetics: 9q22.31
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• MW: 15.9 kDa
• Gene Summary: This gene encodes a member of the class-II pyridoxal-phosphate-dependent aminotransferase family. The encoded protein is the long chain base subunit 1 of serine palmitoyltransferase. Serine palmitoyltransferase converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate and is the key enzyme in sphingolipid biosynthesis. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified. Pseudogenes of this gene have been defined on chromosomes 1, 6, 10, and 13. [provided by RefSeq, Jul 2013]