Von Hippel Lindau (VHL) (NM_000551) Human Tagged ORF Clone Lentiviral Particle

CAT#: RC216151L4V

Lenti ORF particles, VHL (mGFP-tagged) - Human von Hippel-Lindau tumor suppressor (VHL), transcript variant 1, 200ul, >10^7 TU/mL

Product Images

RC216151L4 was used to prepare Lentiviral particles using TR30037 packaging kit. HEK293T cells were transduced with RC216151L4V particle to overexpress human VHL-mGFP fusion protein.

Specifications

Product Data

• Type: Human Tagged ORF Clone Lentiviral Particle
• Tag: mGFP
• Symbol: Von Hippel Lindau
• Synonyms: HRCA1; pVHL; RCA1; VHL1
• Mammalian Cell Selection: Puromycin
• Vector: pLenti-C-mGFP-P2A-Puro
• ACCN: NM_000551
• ORF Size: 639 bp
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC216151).
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_000551.2
• RefSeq Size: 2968 bp
• RefSeq ORF: 642 bp
• Locus ID: 7428
• UniProt ID: P40337
• Cytogenetics: 3p25.3
• Domains: VHL
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis
• MW: 24 kDa
• Gene Summary: Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]