RNase H1 (RNASEH1) (NM_002936) Human Tagged ORF Clone Lentiviral Particle

CAT#: RC200595L2V

Lenti ORF particles, RNASEH1 (mGFP-tagged) - Human ribonuclease H1 (RNASEH1), 200ul, >10^7 TU/mL

Product Images

RC200595L2 was used to prepare Lentiviral particles using TR30037 packaging kit. HEK293T cells were transduced with RC200595L2V particle to overexpress human RNASEH1-mGFP fusion protein.

Specifications

Product Data

• Type: Human Tagged ORF Clone Lentiviral Particle
• Tag: mGFP
• Symbol: RNASEH1
• Synonyms: H1RNA; PEOB2; RNH1
• Mammalian Cell Selection: None
• Vector: pLenti-C-mGFP
• ACCN: NM_002936
• ORF Size: 858 bp
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC200595).
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_002936.3
• RefSeq Size: 1865 bp
• RefSeq ORF: 861 bp
• Locus ID: 246243
• UniProt ID: O60930
• Cytogenetics: 2p25.3
• Domains: rnaseH
• Protein Pathways: DNA replication
• MW: 32.1 kDa
• Gene Summary: This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and plays a key role in DNA replication and repair. Alternate in-frame start codon initiation results in the production of alternate isoforms that are directed to the mitochondria or to the nucleus. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF). Mutations in this gene have been found in individuals with progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2. Alternative splicing results in additional coding and non-coding transcript variants. Pseudogenes of this gene have been defined on chromosomes 2 and 17. [provided by RefSeq, Jul 2017]