HA (NC_007366) Virus Tagged ORF Clone

CAT#: VC102455

Myc-DDK-tagged ORF clone of viral ORF for hemagglutinin [Influenza A virus (A/New York/392/2004(H3N2))], codon optimized for human cell expression, YP_308839

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102455 represents NCBI reference of YP_308839 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACCATCATTGCCCTGAGCTATATCTTGTGTCTCGTTTTCGCGCAGAAGCTTCCGGGAAACGACA
  ATTCTACAGCAACTCTGTGTCTCGGACACCATGCGGTGCCTAACGGAACAATTGTGAAGACCATAACGAA
  CGACCAGATCGAAGTGACAAACGCTACAGAGCTCGTGCAATCTTCCTCCACAGGGGGGATCTGTGACTCA
  CCACATCAAATCCTGGACGGCGAAAACTGCACCCTGATAGACGCCCTGCTGGGTGATCCCCAGTGTGACG
  GGTTCCAGAACAAGAAGTGGGACCTTTTTGTGGAACGGTCTAAGGCATACAGTAATTGCTATCCTTATGA
  CGTCCCCGACTACGCATCCCTCAGGTCACTCGTAGCTAGTTCTGGTACACTGGAGTTCAATAATGAGAGC
  TTCAACTGGACGGGCGTTACCCAAAACGGAACTTCCAGCGCTTGTAAGCGCCGGTCCAATAATAGCTTTT
  TTTCCAGGCTGAACTGGCTGACTCATCTGAAATTTAAGTACCCTGCCCTTAATGTGACCATGCCCAACAA
  CGAGAAGTTCGATAAACTCTATATTTGGGGCGTCCACCATCCAGGGACCGATAACGACCAAATTTCCCTG
  TACGCCCAGGCTTCTGGCCGCATCACAGTATCCACGAAAAGGTCCCAGCAGACAGTTATCCCTTCTATCG
  GCTCCCGCCCACGCATCCGAGACGTGCCTTCCAGGATCTCCATATATTGGACGATCGTCAAGCCCGGAGA
  CATTCTCCTGATTAACTCAACTGGCAATCTCATTGCTCCTAGAGGCTACTTTAAAATCCGGTCCGGCAAA
  TCATCCATCATGCGATCCGACGCCCCAATTGGAAAGTGCAACAGTGAGTGTATCACTCCAAACGGCAGCA
  TCCCTAACGATAAACCCTTTCAGAATGTCAACCGAATCACATACGGAGCTTGCCCACGGTACGTCAAGCA
  GAACACCCTTAAACTGGCCACCGGGATGAGAAACGTCCCGGAAAAACAGACCAGAGGCATCTTCGGCGCT
  ATAGCGGGGTTTATTGAGAACGGGTGGGAAGGTATGGTGGACGGCTGGTATGGCTTCAGACATCAAAATA
  GTGAGGGTACAGGACAGGCCGCCGACCTGAAGTCCACTCAGGCTGCAATTAATCAGATTAATGGCAAGCT
  CAATCGGTTGATCGGGAAGACTAATGAGAAGTTTCACCAAATCGAGAAGGAATTCTCAGAAGTGGAAGGT
  CGGATCCAGGATCTCGAGAAGTACGTTGAAGATACCAAAATTGATCTGTGGTCCTATAACGCGGAGCTGC
  TGGTGGCCCTTGAGAATCAACATACAATCGATTTGACTGACAGCGAGATGAATAAACTGTTTGAACGCAC
  AAAGAAGCAGTTGAGAGAGAACGCCGAAGATATGGGCAATGGCTGTTTCAAGATCTATCACAAGTGTGAT
  AACGCTTGCATCGGATCAATTAGAAACGGGACATACGACCATGATGTCTATAGAGATGAGGCACTGAACA
  ATCGGTTTCAAATCAAGGGCGTCGAACTTAAGTCCGGCTATAAAGACTGGATCTTGTGGATAAGCTTTGC
  TATTTCTTGTTTTTTGCTGTGTGTGGCACTGCTCGGTTTCATCATGTGGGCATGCCAGAAAGGCAATATC
  CGCTGTAACATCTGCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102455 representing YP_308839
  Red=Cloning sites Green=Tags
  
  MKTIIALSYILCLVFAQKLPGNDNSTATLCLGHHAVPNGTIVKTITNDQIEVTNATELVQSSSTGGICDS
  PHQILDGENCTLIDALLGDPQCDGFQNKKWDLFVERSKAYSNCYPYDVPDYASLRSLVASSGTLEFNNES
  FNWTGVTQNGTSSACKRRSNNSFFSRLNWLTHLKFKYPALNVTMPNNEKFDKLYIWGVHHPGTDNDQISL
  YAQASGRITVSTKRSQQTVIPSIGSRPRIRDVPSRISIYWTIVKPGDILLINSTGNLIAPRGYFKIRSGK
  SSIMRSDAPIGKCNSECITPNGSIPNDKPFQNVNRITYGACPRYVKQNTLKLATGMRNVPEKQTRGIFGA
  IAGFIENGWEGMVDGWYGFRHQNSEGTGQAADLKSTQAAINQINGKLNRLIGKTNEKFHQIEKEFSEVEG
  RIQDLEKYVEDTKIDLWSYNAELLVALENQHTIDLTDSEMNKLFERTKKQLRENAEDMGNGCFKIYHKCD
  NACIGSIRNGTYDHDVYRDEALNNRFQIKGVELKSGYKDWILWISFAISCFLLCVALLGFIMWACQKGNI
  RCNICI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007366
• ORF Size: 1698 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007366.1, YP_308839
• RefSeq ORF: 1698 bp
• Locus ID: 3655151
• MW: 63.5 kDa