ORF085R (NC_003494) Virus Tagged ORF Clone

CAT#: VC102375

Myc-DDK-tagged ORF clone of viral ORF for ORF085R [Infectious spleen and kidney necrosis virus], codon optimized for human cell expression, NP_612307

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF085R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102375 represents NCBI reference of NP_612307 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACATAGCATTGTATAGTACTATGCGCTACCGGAACAAGATCAGAAAGATGGACTGCATTTGCCACC
  TTGATTGCTTGTTCACCAACCCCTTCACCAAGTGCTCATGTTCTTCCCATCAACACACCCCTTCATGCCC
  TCAAGCCAAGACTATGTGGTGTGACGATTACCTCACACGACGCCGGCTCGAGCTGATGCCTCCTCCACCT
  CCGCCACCACCCAAGGCCAGTCAGTGCACTGAGGAGTCATCTTCAGGTGGCCAGCCTCCCCCTCCTCCTT
  CACCACTCCCCAAGGCCAGCGAGTGTACGGAAGAGAGCAGCAGCGGCTGGACCTCTTCAGGCAGTGGTTC
  CAGTGCTGACCATGAATCCCGGCGCAAAAGAGGCACCGGAAGGCGGCATCCTTATTCCCGCTCAGAGCGA
  ATTTGCCATAATAATTCCGAGAGGGTGCGGCGGATGCAGTTCAAAGATGATATGGACTGCCTTGCCACGA
  TCATCGAACAGTGCGGACACACCGTGCAACGACGCACAAAGAGAGAAATCATTGCGTCAGCAATTCAGTG
  TTTGCTTAGCATGCAACAGAAGCTGAGGCAAGCTGCTCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102375 representing NP_612307
  Red=Cloning sites Green=Tags
  
  MNIALYSTMRYRNKIRKMDCICHLDCLFTNPFTKCSCSSHQHTPSCPQAKTMWCDDYLTRRRLELMPPPP
  PPPPKASQCTEESSSGGQPPPPPSPLPKASECTEESSSGWTSSGSGSSADHESRRKRGTGRRHPYSRSER
  ICHNNSERVRRMQFKDDMDCLATIIEQCGHTVQRRTKREIIASAIQCLLSMQQKLRQAAQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003494
• ORF Size: 600 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_003494.1, NP_612307
• RefSeq ORF: 600 bp
• Locus ID: 935352
• MW: 22.7 kDa