PB2 (NC_006505) Virus Tagged ORF Clone

CAT#: VC102281

Myc-DDK-tagged ORF clone of viral ORF for PB2 polymerase [Infectious salmon anemia virus], codon optimized for human cell expression, YP_145807

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PB2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102281 represents NCBI reference of YP_145807 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTTCATCAGCGAAAATACCATCAGCGACAAAACTACCCTCGAAGAGCTGAAAAATGCCACTCTGT
  TTCAAGTCACTAAAGTGGACGACCGGGATTGCCTGCGGGCCAGACGGATCTGCAACGCCCCCAAGGGGCA
  TTGGGCCGGACTCATGGAGAAAGCCAAAGCAATGGGGGACCCCACCGAGGAGGAGAAGGACGAGCTCAAG
  AAGATCGTCGAGAGCTACAACACCGTGAGCGTGCTCGGCGTGAGCAAATCCGAAGGCGCAACCGGACCCC
  GGCTCGTGTCCAGCCTCAAGGGCCTGAAGAACCTGCTGCCCGGCGTCAACCCCAAGACCCTGCAGGAGAC
  ACTGCTGGTGGGCGCCCCCTGTCCCAGCACCGAGCCCACCACTGAGGAGTATTGGAATGTGTGCCGGGCC
  GCTGTCGGAGCCTCCATGGGCAGCGCCAAGATCAACATGTCCCAGAAAGTGGTGATGGGCGCCAGCGTCA
  TCGGCTGGGGACAGCTCAATCAGAGCGGCCCCGGCGTGTACTTCCTCAACACCAAGGAGATTGTGACCGC
  CGAAGGCAAGGTGGACGAGACCCGGGGCCCCCTCGAGCGGACCTCCGCCCCCCTCATGCGGGATATCTCC
  CGGCTGATCCAGGAAACCATCGAGGAGGTCGAGACCGGCGGAGATCCCTCCTTTAGCGTGCGGAGCGAGG
  GCGGCTCCAAGATTGAAGGGCGGATCGCCTTCTCCCTGCACTCCGAGGTCTCCACCCTGAAGATGCGGAT
  CGCACTGGAGCAGAAGCTGGCCAAATACGAGTACATGGGCGAGAACCTCCTCACCCTCGTGAAGAACACC
  AGCATCGACCGGATGCAGCCCGACAGCGCCATGATGGGCAAAATGGTGCTCGAGAGCCTGCGGACCCATA
  CCGTCAGCAGCGAGCAGCTCAACGGCCGGATGATCACCGTGCAGAGCCAGGGGCTCGAAACCATTGCAAT
  CAGCAGCCCCTTCGACGTGGAGTACGACGATGGCTACGTCTTCACCCGGATGAAAGGCAACTTCGTGGCT
  GTGGGCCGGGACTACAAGGGCGCCATCCTGTGCTTCCGGGAGGGGCAGGGCACCTTCTTCAGCGGCCGGG
  GCAATTGGAGCGGCCTCATGGAAAAGTGCCTGGTCGAAATGCGGCTCTGCCCCTGCTTCTACAGCTGCAC
  CTGGCAGGACTACCCCGACAAGAAGAGCCTGTACGAGAAAGCCACCTTTGAGGCCAAGCAGATCGTGTTC
  GCCATGGGCGAGAACACCGGCGTGGACATCCGGGTGAACACCGACGGCGAGATCGGCGACAAGGGCATCA
  GCCTGCTGACCCGGGAGCGGGAGGACAAGTACATGAGCAAGGTGTCCTACGAGTGCCGGGTGGTGAGCGG
  CAAGCTGGTGATGGGCCTCGACAAAATGAGCCGGGTCGCCAAGGGCAACCTGGAGGTCGTCCGGGAGAAG
  GGGGACGACACCTCCCAAAGCGACAGCTTCTACGAGGGCGTGCTGCAAGTCGGCAGCATGATTGGCACAA
  CAATGGAAAGCCTGAAGCAGCAGCTGCAGGGCCCCGTGGGCATCTGGCGGGCCTCCGGCGTCTCCGCAAT
  GGAGCGGTGCATGAAGCGGGGCCAGTCCAAAACCGTCGTCGCCTCCGCCCGGTACACCTTCCAGAAGATG
  ATGGAGAAGATGGCCACCGGGCGGGAGGTCTCCAAGTACTCCCTGATCATCGTCATGCGGTGCTGCATCG
  GCTTTACCAGCGAGGCCAACAAGCGGGCCCTGACCAACATCAGCGGCACCGGGTACTACATCAGCGTGGC
  CCAGCCCACCGTGGTGAAGCTGGCCGGCGAGTGGCTCATCACCCCCGTCGGCCGGAGCAAGACCGGGGAA
  GTGCAATACGTCAGCGCCAAGCTGAAGAAGGGGATGACCACCGGCAAGCTCGAGCTCATCAAGAAGGCCG
  ACCGGTCCGACCTCGACAACTTCCCCGAGCCAAGCGCCGACGAGCTGCTGCGGGAAGGCACCATTGTGCT
  GATGCAGATCGGCAAGGACAAATGGCTGTGCCGGGTGCGGACCGGCGACCGGCGGGTGCGGACCGACACA
  GACATTCAGCGGGCCGAGGCCAAGTCCCAGGTCGAGAAGGAGGACCTCATGGACGAGTACGGGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102281 representing YP_145807
  Red=Cloning sites Green=Tags
  
  MDFISENTISDKTTLEELKNATLFQVTKVDDRDCLRARRICNAPKGHWAGLMEKAKAMGDPTEEEKDELK
  KIVESYNTVSVLGVSKSEGATGPRLVSSLKGLKNLLPGVNPKTLQETLLVGAPCPSTEPTTEEYWNVCRA
  AVGASMGSAKINMSQKVVMGASVIGWGQLNQSGPGVYFLNTKEIVTAEGKVDETRGPLERTSAPLMRDIS
  RLIQETIEEVETGGDPSFSVRSEGGSKIEGRIAFSLHSEVSTLKMRIALEQKLAKYEYMGENLLTLVKNT
  SIDRMQPDSAMMGKMVLESLRTHTVSSEQLNGRMITVQSQGLETIAISSPFDVEYDDGYVFTRMKGNFVA
  VGRDYKGAILCFREGQGTFFSGRGNWSGLMEKCLVEMRLCPCFYSCTWQDYPDKKSLYEKATFEAKQIVF
  AMGENTGVDIRVNTDGEIGDKGISLLTREREDKYMSKVSYECRVVSGKLVMGLDKMSRVAKGNLEVVREK
  GDDTSQSDSFYEGVLQVGSMIGTTMESLKQQLQGPVGIWRASGVSAMERCMKRGQSKTVVASARYTFQKM
  MEKMATGREVSKYSLIIVMRCCIGFTSEANKRALTNISGTGYYISVAQPTVVKLAGEWLITPVGRSKTGE
  VQYVSAKLKKGMTTGKLELIKKADRSDLDNFPEPSADELLREGTIVLMQIGKDKWLCRVRTGDRRVRTDT
  DIQRAEAKSQVEKEDLMDEYGV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006505
• ORF Size: 2166 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_006505.1, YP_145807
• RefSeq ORF: 2166 bp
• Locus ID: 3170818
• MW: 79.9 kDa