gag-pro (NC_001436) Virus Tagged ORF Clone

CAT#: VC102142

Myc-DDK-tagged ORF clone of viral ORF for Pr gag-pro [Human T-lymphotropic virus 1], codon optimized for human cell expression, NP_057861

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: gag-pro
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102142 represents NCBI reference of NP_057861 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCAAATTTTCAGCCGGTCTGCAAGTCCTATTCCTAGACCTCCACGCGGCTTGGCTGCCCATCACT
  GGTTGAACTTCCTGCAGGCAGCTTATAGGCTGGAGCCAGGCCCTAGTTCTTACGATTTTCACCAGCTGAA
  AAAGTTCCTCAAGATCGCCCTGGAAACACCAGTTTGGATTTGCCCTATTAACTATTCTCTCCTGGCCTCT
  CTTCTTCCCAAGGGATATCCCGGCAGAGTGAACGAGATCCTGCACATCCTGATACAGACACAGGCTCAGA
  TTCCCAGTAGGCCAGCTCCTCCGCCACCCTCTTCCTCCACACATGACCCACCCGATAGCGACCCACAGAT
  CCCACCGCCGTACGTGGAGCCCACAGCTCCCCAGGTGCTCCCCGTGATGCACCCTCACGGTGCACCTCCA
  AACCACAGGCCATGGCAGATGAAGGATCTGCAGGCGATCAAACAAGAAGTTAGCCAGGCAGCTCCGGGGT
  CACCACAATTTATGCAGACGATCCGCCTTGCCGTCCAGCAGTTTGACCCCACAGCCAAGGACCTCCAGGA
  TTTGCTGCAATACCTTTGCTCTTCTCTCGTCGCTTCTCTGCATCACCAGCAACTTGATAGCCTTATTAGC
  GAGGCCGAGACCCGGGGCATCACTGGATATAACCCACTCGCGGGTCCCCTCAGAGTCCAGGCCAACAACC
  CCCAGCAGCAAGGTCTGAGACGCGAATACCAACAGCTCTGGCTTGCTGCCTTTGCCGCTCTGCCAGGAAG
  CGCCAAAGATCCTTCCTGGGCATCCATCCTGCAGGGACTGGAGGAGCCCTACCACGCCTTTGTCGAAAGA
  CTGAATATCGCCCTGGATAACGGCCTTCCTGAGGGCACCCCTAAAGATCCCATCCTCAGAAGCCTCGCCT
  ACAGCAACGCTAATAAAGAATGCCAGAAACTGCTTCAGGCCAGAGGCCATACCAACTCACCGCTCGGCGA
  CATGCTGAGAGCTTGCCAGGCTTGGACCCCTAAAGACAAAACCAAGGTCCTGGTAGTTCAGCCAAAAAAG
  CCCCCTCCAAACCAGCCCTGCTTCAGGTGCGGGAAAGCCGGGCATTGGTCTCGGGACTGTACCCAACCCA
  GGCCGCCTCCTGGCCCATGTCCTCTCTGCCAAGACCCAACGCACTGGAAACGGGATTGCCCCCGACTTAA
  GCCTACCATCCCCGAGCCCGAACCCGAAGAGGACGCCCTTCTGCTTGACCTTCCTGCCGACATCCCGCAC
  CCTAAGAATCTTCACAGGGGAGGAGGCCTTACATCTCCCCCAACTTTGCAACAGGTGCTGCCGAATCAGG
  ATCCAACATCTATCCTCCCGGTTATACCACTTGATCCAGCGAGAAGACCCGTGATTAAAGCACAGATTGA
  CACACAGACCAGCCACCCCAAAACAATAGAGGCCTTGCTGGACACCGGGGCCGATATGACGGTGCTCCCA
  ATTGCTCTGTTTAGTAGTAATACCCCACTGAAGAACACCAGCGTTCTGGGCGCAGGCGGCCAGACCCAGG
  ACCACTTCAAATTGACCTCCCTCCCTGTGCTGATAAGGTTGCCGTTTAGAACCACTCCTATTGTTCTTAC
  CTCATGTCTCGTCGACACCAAGAACAACTGGGCGATTATTGGGCGAGACGCTCTGCAACAGTGTCAGGGG
  GTCCTGTACCTCCCCGAGGCTAAACGACCCCCCGTTATATTGCCTATCCAGGCCCCCGCGGTCCTGGGGC
  TTGAACACCTTCCTAGACCTCCCGAGATTAGCCAGTTTCCCCTGAATCAGAATGCCTCCAGACCATGTAA
  CACGTGGAGCGGCCGCCCATGGAGACAAGCGATTAGCAATCCCACACCAGGGCAAGAGATAACACAATAC
  AGCCAGCTGAAGAAGCCTATGGAGCCTGGAGATAGTTCAACCACTTGCGGGCCTCTGACACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102142 representing NP_057861
  Red=Cloning sites Green=Tags
  
  MGQIFSRSASPIPRPPRGLAAHHWLNFLQAAYRLEPGPSSYDFHQLKKFLKIALETPVWICPINYSLLAS
  LLPKGYPGRVNEILHILIQTQAQIPSRPAPPPPSSSTHDPPDSDPQIPPPYVEPTAPQVLPVMHPHGAPP
  NHRPWQMKDLQAIKQEVSQAAPGSPQFMQTIRLAVQQFDPTAKDLQDLLQYLCSSLVASLHHQQLDSLIS
  EAETRGITGYNPLAGPLRVQANNPQQQGLRREYQQLWLAAFAALPGSAKDPSWASILQGLEEPYHAFVER
  LNIALDNGLPEGTPKDPILRSLAYSNANKECQKLLQARGHTNSPLGDMLRACQAWTPKDKTKVLVVQPKK
  PPPNQPCFRCGKAGHWSRDCTQPRPPPGPCPLCQDPTHWKRDCPRLKPTIPEPEPEEDALLLDLPADIPH
  PKNLHRGGGLTSPPTLQQVLPNQDPTSILPVIPLDPARRPVIKAQIDTQTSHPKTIEALLDTGADMTVLP
  IALFSSNTPLKNTSVLGAGGQTQDHFKLTSLPVLIRLPFRTTPIVLTSCLVDTKNNWAIIGRDALQQCQG
  VLYLPEAKRPPVILPIQAPAVLGLEHLPRPPEISQFPLNQNASRPCNTWSGRPWRQAISNPTPGQEITQY
  SQLKKPMEPGDSSTTCGPLTL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001436
• ORF Size: 1953 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001436.1, NP_057861
• RefSeq ORF: 1953 bp
• MW: 71.7 kDa