F (NC_003443) Virus Tagged ORF Clone

CAT#: VC102055

Myc-DDK-tagged ORF clone of viral ORF for fusion protein [Human parainfluenza virus 2], codon optimized for human cell expression, NP_598404

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: F Protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102055 represents NCBI reference of NP_598404 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCATCTCCACCCTATGATTGTCTGTATTTTCGTCATGTATACCGGCATCGTGGGCAGTGATGCCA
  TAGCCGGGGACCAGCTTCTCAATATTGGTGTCATCCAATCTAAAATTCGCAGCCTGATGTATTACACCGA
  TGGGGGCGCCTCTTTCATCGTTGTTAAACTCCTGCCCAACCTGCCCCCCTCTAATGGCACCTGCAATATC
  ACTAGTCTTGACGCCTATAATGTGACACTCTTCAAACTGCTCACACCTCTGATAGAAAATCTGAGCAAGA
  TTAGTACCGTGACCGACACCAAGACACGCCAGAAGAGATTTGCCGGAGTCGTGGTGGGGCTGGCTGCCCT
  GGGTGTAGCGACCGCTGCTCAGATCACCGCCGCCGTGGCGATTGTGAAAGCGAATGCAAATGCCGCGGCT
  ATTAACAACCTGGCGTCATCCATCCAATCCACTAACAAAGCGGTGTCCGATGTGATTGATGCTTCTCGCA
  CCATTGCTACTGCTGTGCAGGCCATACAAGATCGAATCAACGGTGCTATCGTAAACGGAATCACTAGCGC
  TAGCTGCAGGGCTCATGACGCCTTGATCGGCAGTATCCTGAATCTGTATCTGACTGAGCTGACCACCATC
  TTCCACAACCAGATAACGAACCCCGCTCTGACACCACTCTCCATCCAAGCGCTGAGGATCCTTCTCGGGA
  GCACTTTGCCAATCGTGATCGAGAGCAAGCTGAACACCAACTTCAATACTGCAGAGCTCCTGAGTTCCGG
  CTTGCTGACGGGCCAGATCATCTCAATTTCACCAATGTACATGCAGATGCTGATCCAAATAAACGTGCCC
  ACCTTCATCATGCAGCCTGGGGCTAAAGTGATTGACTTGATCGCCATAAGCGCTAATCACAAACTGCAGG
  AGGTGGTGGTGCAAGTGCCTAACCGGATTCTCGAATATGCTAATGAACTGCAGAATTACCCCGCCAACGA
  TTGTGTGGTCACACCTAATTCCGTGTTCTGCAGATACAATGAAGGCAGTCCCATTCCCGAGAGTCAGTAC
  CAGTGTCTTCGGGGCAATCTGAACTCATGCACCTTCACTCCCATTATTGGAAATTTTCTGAAGCGATTCG
  CCTTCGCCAATGGAGTCCTTTACGCCAATTGCAAATCTCTTCTGTGTAGGTGTGCAGACCCCCCACACGT
  CGTCTCACAAGACGATACACAAGGAATATCTATCATCGATATCAAGAGATGCTCCGAGATGATGCTCGAT
  ACCTTTAGCTTTCGAATCACATCAACCTTTAATGCTACATACGTTACTGACTTCAGTATGATAAATGCCA
  ACATTGTCCATCTTAGTCCCCTCGACCTGTCTAATCAGATTAATTCAATCAATAAATCCCTCAAGAGCGC
  GGAAGACTGGATCGCCGACTCAAATTTCTTCGCGAACCAAGCCAGGACAGCTAAGACATTGTACTCCTTG
  AGCGCCATAGCCCTGATACTCTCAGTGATCACACTGGTGGTTGTTGGCCTGCTTATCGCTTACATCATTA
  AACTGGTGAGTCAAATACACCAATTCCGCTCCCTTGCAGCGACCACAATGTTTCATAGAGAAAACCCCGC
  ATTCTTTTCCAAAAATAATCATGGAAACATCTATGGCATTAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102055 representing NP_598404
  Red=Cloning sites Green=Tags
  
  MHHLHPMIVCIFVMYTGIVGSDAIAGDQLLNIGVIQSKIRSLMYYTDGGASFIVVKLLPNLPPSNGTCNI
  TSLDAYNVTLFKLLTPLIENLSKISTVTDTKTRQKRFAGVVVGLAALGVATAAQITAAVAIVKANANAAA
  INNLASSIQSTNKAVSDVIDASRTIATAVQAIQDRINGAIVNGITSASCRAHDALIGSILNLYLTELTTI
  FHNQITNPALTPLSIQALRILLGSTLPIVIESKLNTNFNTAELLSSGLLTGQIISISPMYMQMLIQINVP
  TFIMQPGAKVIDLIAISANHKLQEVVVQVPNRILEYANELQNYPANDCVVTPNSVFCRYNEGSPIPESQY
  QCLRGNLNSCTFTPIIGNFLKRFAFANGVLYANCKSLLCRCADPPHVVSQDDTQGISIIDIKRCSEMMLD
  TFSFRITSTFNATYVTDFSMINANIVHLSPLDLSNQINSINKSLKSAEDWIADSNFFANQARTAKTLYSL
  SAIALILSVITLVVVGLLIAYIIKLVSQIHQFRSLAATTMFHRENPAFFSKNNHGNIYGIS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_003443
• ORF Size: 1653 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_003443.1, NP_598404
• RefSeq ORF: 1653 bp
• Locus ID: 935186
• MW: 59.7 kDa