ORF E1 (NC_001354) Virus Tagged ORF Clone

CAT#: VC101934

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein [Human papillomavirus type 41], codon optimized for human cell expression, NP_040287

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101934 represents NCBI reference of NP_040287 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCCCGGGTATCAGACACTGGAAATGGCAATGAGAACAAGGAGAATGAGGGGACCGTAGCCAGCG
  ATCATAGCGAAGCCCGGTGTAGTTACATCCTGTTCGAGGCTGAATGCTCAGACGGAGGCGACGATGAGGA
  GTCCATGGAGGACAGTCTGGTCGAGGACCTCGTAGATGATGCTAGCGTCCACCAGGGTAACTCACTGAGC
  CTGTTCCATGCCCAGACCGTAGAAGAATACGAGGGCGAAATACAGAGCCTTAAGCGGAAATTCATCCTGT
  CCCCACTGCACAGAGATGTCGCCGAACTCTCCCCCAGGCTGGCCGGTGTGTCCCTCGAAGAAAATAGGGG
  GAAAAAAGCCCGCAAGTCCCTTTTTCATGACGATTCCGGCATCGATTCCAGTGCAGTCGAGGTGTCACAG
  CTCTCCAGCACTCCGTCCGCGCCCGGCCCTGATATCCGGCTGCCCAAGCCCTCTGACATTGATCTCGAGC
  CTCTCTTCCAGTCAAGACAGCGGTGCACTCACATGTATAGTAAGTTTAAGGCTGTATACGGGGTAAGCTT
  TACTGATATTACAAGGCCGTTCAAGAGCGATAAGACCACAAGTCAGCACTGGGTGGTGGCTGCCTACTAT
  CTGGCGTTTGATTCAGAAATCAGTGCCATGGAAGTGCTGCTCCGACAGCAGTGTCAGTTCCTGTATATCG
  ACAATAACGACGGTATCATACTGTTCTTTCTGGAGTACAATGTGCAAAAATCTCGAACTACCGTCTATAA
  TTGGTTCACTGCCAACTTTCATTACAACGAAAATAGGATGCTGGCCAATCCACCCAGAACCAGAAACATG
  CCTGCAGCCCTGTTTTTCTACCACCGATTTATGGGCACCGGCGGTATCAAGCACGGCGCGATGCCAGAAA
  TCATTGTTAATCAGTGCGTGGTCAGTAACCAGCAGACCGACACCTTCGAGCTTTCCCGCATGGTCCAGTG
  GGCTCTGGATAATGACCTGCAGGACGAGCACATGTTGGCACTCGAGTACGCATTGTTGGCCGAGAGCGAC
  GGGAACGCGAGAGCCTTCCTCAAACAGAACAACCAGCCAATGATTGTGAAAAATTGCTCCATTATGGTTA
  GACATTATAAAACTGCGTTGGTGGCGAAAATGTCAATCTCACAATACGTTAATAAAAGGTGCCTGGATCA
  CGGGGAGGCGGACGAAAATTCCTGGAGGGGAATTGTGCATTTCCTGAGATACCAGGGCCAGGAGTTCCTC
  CCTTTTATGTGCAAGATGCATAACTTCCTGCACCACAGACCGAAAAAGAGCACACTTGTCCTCTGTGGTC
  CATCTGACACCGGCAAATCTTACTTTGCTAATGGGTTGAACAAGTTCCTGGACGGGCACGTACTGTCCTT
  CGTAAGTAACGGTAGTCACTTCTGGCTCTCCCCACTTCGAGGCGCCCGGTGTTGCTTGATTGACGACGCC
  ACCCTCACATTCTGGCGGTATGCAGATCAGAACATGAGGGCACTGCTGGATGGATATGAAATTAGTATCG
  ATGCCAAACACCGAAATCCCATGCAGACAAGGGCACCACCTCTGATAATCACAACCAACGAAGATATTAT
  GAGGCTGGACGAATTTAAGTACCTCCAGACCAGAACAATGTACGTGTACTTTAATAAACCCTTCCCACTG
  AAGGGCAACGGACAGCCACTCTATTATATAGACGGATATACGTGGAACAGCTTCTTCCGGAAATTCTGGC
  GACACCTTAATCTCAAGGATCCGGAAGATGAAAGCGACGGTGAAACTCCTGGCACCATCCGACTGTATAC
  GCGAGCCGATACTGATACTATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101934 representing NP_040287
  Red=Cloning sites Green=Tags
  
  MASRVSDTGNGNENKENEGTVASDHSEARCSYILFEAECSDGGDDEESMEDSLVEDLVDDASVHQGNSLS
  LFHAQTVEEYEGEIQSLKRKFILSPLHRDVAELSPRLAGVSLEENRGKKARKSLFHDDSGIDSSAVEVSQ
  LSSTPSAPGPDIRLPKPSDIDLEPLFQSRQRCTHMYSKFKAVYGVSFTDITRPFKSDKTTSQHWVVAAYY
  LAFDSEISAMEVLLRQQCQFLYIDNNDGIILFFLEYNVQKSRTTVYNWFTANFHYNENRMLANPPRTRNM
  PAALFFYHRFMGTGGIKHGAMPEIIVNQCVVSNQQTDTFELSRMVQWALDNDLQDEHMLALEYALLAESD
  GNARAFLKQNNQPMIVKNCSIMVRHYKTALVAKMSISQYVNKRCLDHGEADENSWRGIVHFLRYQGQEFL
  PFMCKMHNFLHHRPKKSTLVLCGPSDTGKSYFANGLNKFLDGHVLSFVSNGSHFWLSPLRGARCCLIDDA
  TLTFWRYADQNMRALLDGYEISIDAKHRNPMQTRAPPLIITTNEDIMRLDEFKYLQTRTMYVYFNKPFPL
  KGNGQPLYYIDGYTWNSFFRKFWRHLNLKDPEDESDGETPGTIRLYTRADTDTI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001354
• ORF Size: 1842 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001354.1, NP_040287
• RefSeq ORF: 1842 bp
• Locus ID: 1489277
• MW: 70.3 kDa