L1 (NC_001526) Virus Tagged ORF Clone

CAT#: VC101906

Myc-DDK-tagged ORF clone of viral ORF for major capsid L1 protein [Human papillomavirus type 16], codon optimized for human cell expression, NP_041332

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101906 represents NCBI reference of NP_041332 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAGTTACTTTCATCTACATTCTCGTTATTACATGTTATGAGAATGACGTGAATGTGTACCACATCT
  TTTTTCAGATGAGCCTGTGGCTCCCAAGTGAAGCAACAGTTTACCTGCCACCCGTGCCAGTAAGTAAGGT
  CGTGAGTACGGATGAGTACGTGGCACGCACGAATATTTACTATCACGCTGGTACTAGTAGACTTTTGGCT
  GTGGGGCACCCCTACTTTCCTATCAAAAAGCCTAACAACAACAAGATACTTGTGCCAAAGGTGAGCGGCC
  TCCAATACAGGGTGTTTCGCATCCACTTGCCTGACCCTAACAAATTCGGATTTCCCGATACTTCATTTTA
  CAATCCCGACACACAGCGGCTTGTGTGGGCTTGTGTTGGTGTAGAGGTGGGACGGGGGCAGCCACTGGGG
  GTGGGGATCAGTGGACACCCGCTTCTGAACAAGCTCGACGACACTGAGAATGCTAGCGCGTATGCAGCAA
  ATGCAGGGGTTGATAATAGAGAGTGTATCAGCATGGACTACAAGCAGACTCAACTCTGCCTGATTGGCTG
  CAAACCCCCGATCGGCGAGCACTGGGGAAAAGGTTCTCCTTGTACAAATGTTGCTGTGAACCCAGGTGAT
  TGTCCACCTCTTGAGCTGATTAATACAGTGATCCAGGATGGGGATATGGTGCATACTGGATTTGGTGCAA
  TGGATTTCACCACTCTCCAGGCAAATAAAAGTGAGGTTCCGCTCGATATTTGCACAAGCATTTGCAAATA
  CCCAGATTATATCAAGATGGTGAGCGAGCCCTACGGTGATTCCCTCTTCTTCTATCTGAGGCGCGAGCAG
  ATGTTCGTTAGGCATCTCTTTAACCGGGCTGGTACAGTTGGGGAAAATGTGCCCGACGACCTGTACATCA
  AAGGCTCTGGCAGCACTGCTAATCTCGCAAGCAGTAACTACTTTCCAACACCGTCTGGGTCCATGGTGAC
  CTCTGACGCCCAGATCTTTAACAAACCCTACTGGCTCCAAAGAGCTCAGGGACATAATAATGGCATCTGT
  TGGGGCAATCAGCTTTTCGTGACGGTGGTCGACACGACTAGGTCTACCAACATGAGCCTCTGTGCCGCGA
  TCAGTACATCAGAAACAACTTATAAAAACACAAATTTTAAAGAGTACCTGCGACATGGCGAAGAGTACGA
  CCTGCAATTTATTTTTCAGCTGTGCAAGATCACTCTGACAGCCGACGTTATGACGTACATCCACTCAATG
  AATTCCACAATCTTGGAGGATTGGAATTTTGGGCTGCAGCCACCACCGGGCGGGACCCTGGAGGACACTT
  ACCGCTTTGTCACTCAGGCAATTGCCTGCCAGAAACACACACCTCCAGCGCCTAAGGAGGACGACCCACT
  GAAAAAGTACACATTCTGGGAGGTGAACCTGAAAGAGAAGTTCAGCGCAGACCTTGACCAGTTCCCTCTT
  GGCAGAAAGTTTCTGCTTCAGGCCGGACTCAAGGCAAAGCCCAAATTCACACTGGGGAAGAGAAAAGCCA
  CACCAACTACAAGTTCTACCAGCACTACTGCCAAACGCAAGAAACGAAAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001526
• ORF Size: 1593 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001526.2, NP_041332
• RefSeq ORF: 1593 bp
• Locus ID: 1489082
• UniProt ID: P03120
• MW: 59.6 kDa