vif (NC_001802) Virus Tagged ORF Clone

CAT#: VC101719

Myc-DDK-tagged ORF clone of viral ORF for Vif [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057851

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: vif
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101719 represents NCBI reference of NP_057851 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAACAGGTGGCAAGTGATGATTGTCTGGCAAGTGGACCGCATGCGAATTAGGACGTGGAAGAGCT
  TGGTGAAACACCATATGTACGTAAGTGGCAAGGCTCGGGGATGGTTCTACAGACATCACTACGAATCTCC
  ACACCCTCGAATTTCTTCCGAGGTACATATCCCGCTCGGGGATGCGCGATTGGTCATTACGACCTACTGG
  GGCCTCCATACCGGCGAGCGGGACTGGCACCTGGGCCAGGGCGTTTCTATTGAGTGGCGAAAAAAGCGGT
  ACTCTACTCAGGTAGACCCCGAACTGGCAGACCAGCTGATACATCTGTATTATTTTGATTGCTTTAGTGA
  CTCCGCGATCCGGAAGGCCCTCCTTGGCCATATCGTCTCCCCCAGGTGCGAGTACCAGGCCGGCCATAAT
  AAAGTGGGTAGTCTGCAGTATCTGGCTTTGGCCGCACTGATTACTCCCAAGAAGATTAAACCCCCTCTCC
  CATCAGTAACTAAGCTTACGGAAGACAGATGGAACAAACCCCAAAAGACAAAAGGACATCGCGGGAGTCA
  TACAATGAATGGACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101719 representing NP_057851
  Red=Cloning sites Green=Tags
  
  MENRWQVMIVWQVDRMRIRTWKSLVKHHMYVSGKARGWFYRHHYESPHPRISSEVHIPLGDARLVITTYW
  GLHTGERDWHLGQGVSIEWRKKRYSTQVDPELADQLIHLYYFDCFSDSAIRKALLGHIVSPRCEYQAGHN
  KVGSLQYLALAALITPKKIKPPLPSVTKLTEDRWNKPQKTKGHRGSHTMNGH
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001802
• ORF Size: 576 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001802.1, NP_057851
• RefSeq ORF: 576 bp
• Locus ID: 155459
• UniProt ID: P04585
• MW: 22.5 kDa