U10 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101358

Myc-DDK-tagged ORF clone of viral ORF for protein UL31 [Human herpesvirus 6], codon optimized for human cell expression, NP_042901

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101358 represents NCBI reference of NP_042901 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAATCGTAACTTATAAGACCGTCAGTGCGCGCTCCCCAACCGTGACATGGTCTAGTGGCTTTGGCA
  GAGCCATCGCATCTATTCAGAAAAGAAACCAGGACAATATTCGGAAACCCCTCCGATTTTACACCGGACT
  CCTGCATTGTCTCATCAAGCAGTACGAGCATTGTCTTGTTCCACCCAATAAGTCTATCCGCTTCGATAAG
  GGCAAGATTGAGGTTGCTGCTCTGATTCTTGATCTGGGCCATCAGGTCCTTGGACGGCAAATACATGTGC
  GCCAACGGATCTATTCTTGGACTTCCATAACCCTTCCTAAGTTGTTCACTCCAAAAGAACTCTACTTCCT
  GATCGCATCTCCCGAAGAGGAAAATATCGCTTTCAATCCGACAATTACTAAGGGAGGATGGATCAGCGGT
  AGTTTCTCATACCCAGTAGATTACAGAAGCAATTTCTCCCTTACTGGGATGTCTGCAAACATTCTCATGG
  TCCCATTTGTCCCCTATAAATATCCTCTCAACTACGCTCGGTTTATCTCCTCCATCGATCTTATGATCCT
  TAATGAGCAGTTCCCAGAACATGAATGTGGGGACATTCAAATTCTGAAACAACGCAACTATCTGTACCTG
  GGTGTCATCAAAAATCTTACCTGGAAGAAATCAGTGACGGGAACGGGTCAGACGGCACCGCACAGAATTC
  TGAAGGCGTCCTTCATCGGTAGCTGGCCTGACACTTCACTGCCCGATCGAGTCGCATTGAGATTTTTTAA
  CAACACACGCTTCACAATACATTGTCACGAGTTCTCAATCAATATCGAGAATTTGGGCCTGGTCAAGAAT
  AAAGAAAAGGTATTTGGGACTCTCGCCACAGTGTGCTGCGAGCAGATTCCTTCACTCCTTACTACCGAGA
  ATCTCCCTAAATACCTTATTGTGCAATTTGAAGTTGTGACCCAAATTGAAGAGCCGGAGCCCTTGCTCTT
  CTCCAGTAATCCTAAGCTTTATTTTACAGGTGATGTGCTGAATGCTACTATCCAGCTGCAACACAATCCA
  AATTACTACGAGTTGCTGGTTCACGCCCCATACGATATTCATTTCTACCCTTCCCGGTGTCACATTGTGA
  TTCTGCCCATCCGGTATTTTACCAAACCCGACAAACAAATCCTTATCTCCGGGTACCAGAATGAAGGCTT
  CTTCGAAACTCAAGTCATGCTGTGGGCACCAGGGACTCCCCTCCACATAACTTTGAGATCCTTTTCACCC
  AATCTGATTCTGCCTCAGAGCACCAAGCGGAGGAACAGGAAAGCAGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101358 representing NP_042901
  Red=Cloning sites Green=Tags
  
  MEIVTYKTVSARSPTVTWSSGFGRAIASIQKRNQDNIRKPLRFYTGLLHCLIKQYEHCLVPPNKSIRFDK
  GKIEVAALILDLGHQVLGRQIHVRQRIYSWTSITLPKLFTPKELYFLIASPEEENIAFNPTITKGGWISG
  SFSYPVDYRSNFSLTGMSANILMVPFVPYKYPLNYARFISSIDLMILNEQFPEHECGDIQILKQRNYLYL
  GVIKNLTWKKSVTGTGQTAPHRILKASFIGSWPDTSLPDRVALRFFNNTRFTIHCHEFSINIENLGLVKN
  KEKVFGTLATVCCEQIPSLLTTENLPKYLIVQFEVVTQIEEPEPLLFSSNPKLYFTGDVLNATIQLQHNP
  NYYELLVHAPYDIHFYPSRCHIVILPIRYFTKPDKQILISGYQNEGFFETQVMLWAPGTPLHITLRSFSP
  NLILPQSTKRRNRKAV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 1308 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001664.2, NP_042901
• RefSeq ORF: 1308 bp
• Locus ID: 1487889
• MW: 50.1 kDa