UL15 (NC_001798) Virus Tagged ORF Clone

CAT#: VC100879

Myc-DDK-tagged ORF clone of viral ORF for DNA packaging terminase subunit 1 [Human herpesvirus 2], codon optimized for human cell expression, NP_044484

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100879 represents NCBI reference of NP_044484 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGGTCAGCAGCTCGCCAGCGATGTACAGCAATACTTGGAACGGCTTGAGAAACAAAGGCAGCAGA
  AAGTTGGGGTTGATGAAGCTTCTGCCGGACTTACCCTGGGCGGAGACGCACTGAGAGTGCCCTTCCTGGA
  CTTCGCAACAGCCACACCTAAACGACACCAGACAGTGGTGCCCGGCGTGGGGACACTTCATGACTGCTGT
  GAGCACAGCCCACTGTTCAGCGCCGTCGCGCGGAGACTGCTCTTCAACTCTCTGGTACCCGCACAGCTGC
  GCGGTAGGGATTTCGGAGGAGATCACACCGCTAAGCTCGAATTTTTGGCTCCCGAACTCGTCAGAGCAGT
  GGCCAGGCTCCGATTTCGCGAGTGCGCTCCGGAAGACGCGGTGCCCCAGCGAAACGCCTACTATTCTGTG
  CTCAACACGTTTCAAGCCCTGCATCGATCCGAAGCCTTTCGACAACTGGTCCATTTCGTCCGGGACTTCG
  CGCAATTGCTTAAAACTAGCTTCCGGGCGTCAAGTCTCGCAGAGACCACTGGCCCACCAAAAAAACGCGC
  TAAAGTGGATGTCGCAACACATGGGCAGACCTACGGTACACTGGAACTTTTTCAGAAGATGATCCTCATG
  CATGCAACCTACTTCCTTGCCGCCGTTCTTCTGGGAGACCATGCTGAGCAAGTCAACACATTCCTCAGGC
  TGGTCTTTGAGATCCCGCTCTTTAGTGATACGGCCGTCCGCCACTTCCGGCAGAGAGCAACCGTGTTTTT
  GGTACCTAGACGCCATGGCAAGACTTGGTTCCTTGTGCCTCTGATCGCCCTGTCACTCGCAAGTTTTCGA
  GGCATCAAGATCGGCTACACGGCACACATCCGAAAAGCCACTGAGCCTGTCTTCGATGAAATCGATGCCT
  GTCTCAGGGGATGGTTCGGAAGCTCTCGCGTGGATCATGTCAAAGGTGAGACAATTTCCTTCAGTTTTCC
  TGACGGATCCCGGTCCACCATTGTTTTCGCCTCATCCCATAATACAAACGGCATCCGGGGGCAAGATTTT
  AATCTCCTGTTCGTGGATGAGGCTAACTTCATTAGGCCGGATGCTGTGCAGACTATCATGGGCTTCCTTA
  ACCAGGCAAATTGCAAGATTATATTCGTGTCTTCAACTAATACGGGTAAAGCCTCTACATCCTTCCTGTA
  TAACCTTAGGGGAGCAGCTGATGAACTGCTTAACGTTGTCACATATATTTGTGATGACCACATGCCAAGG
  GTCGTGACACACACCAATGCGACTGCTTGTAGCTGTTACATTCTCAATAAGCCGGTCTTCATAACTATGG
  ACGGTGCCGTGAGGCGCACGGCGGACTTGTTCTTGCCTGATTCATTCATGCAGGAAATTATCGGGGGCCA
  GGCCCGAGAAACAGGAGACGACCGCCCGGTGCTTACGAAATCCGCAGGTGAGAGGTTCCTCCTTTACAGG
  CCTTCTACTACCACAAATAGCGGACTGATGGCACCAGAGTTGTATGTTTACGTTGACCCGGCCTTCACCG
  CCAACACTAGAGCCTCTGGGACCGGAATCGCTGTTGTCGGAAGATATAGAGATGACTTCATCATTTTTGC
  CCTGGAGCACTTTTTCCTCAGGGCACTTACCGGCAGCGCTCCCGCTGACATCGCTAGATGCGTCGTGCAC
  AGCCTCGCCCAGGTGCTTGCCCTGCATCCAGGCGCGTTTCGGTCCGTGCGGGTGGCTGTGGAGGGAAACA
  GTTCCCAGGACAGCGCTGTCGCTATCGCTACACACGTTCATACTGAGATGCACAGAATCCTCGCTTCAGC
  CGGCGCTAACGGCCCAGGCCCTGAGCTGCTGTTCTATCATTGCGAACCACCAGGCGGAGCAGTGCTTTAT
  CCCTTCTTTCTGCTTAACAAGCAGAAGACCCCCGCTTTTGAATATTTTATCAAGAAATTCAACAGCGGTG
  GCGTGATGGCCAGCCAGGAACTGGTGTCTGTGACTGTGCGACTGCAAACGGACCCCGTGGAGTATCTGAG
  TGAGCAGCTGAACAATCTCATTGAGACCGTGAGCCCTAACACTGATGTGCGAATGTACTCCGGGAAGAGA
  AACGGAGCGGCAGACGATCTGATGGTGGCCGTGATTATGGCTATTTATCTGGCAGCCCCTACTGGAATCC
  CCCCCGCCTTCTTCCCCATCACGAGAACTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100879 representing NP_044484
  Red=Cloning sites Green=Tags
  
  MFGQQLASDVQQYLERLEKQRQQKVGVDEASAGLTLGGDALRVPFLDFATATPKRHQTVVPGVGTLHDCC
  EHSPLFSAVARRLLFNSLVPAQLRGRDFGGDHTAKLEFLAPELVRAVARLRFRECAPEDAVPQRNAYYSV
  LNTFQALHRSEAFRQLVHFVRDFAQLLKTSFRASSLAETTGPPKKRAKVDVATHGQTYGTLELFQKMILM
  HATYFLAAVLLGDHAEQVNTFLRLVFEIPLFSDTAVRHFRQRATVFLVPRRHGKTWFLVPLIALSLASFR
  GIKIGYTAHIRKATEPVFDEIDACLRGWFGSSRVDHVKGETISFSFPDGSRSTIVFASSHNTNGIRGQDF
  NLLFVDEANFIRPDAVQTIMGFLNQANCKIIFVSSTNTGKASTSFLYNLRGAADELLNVVTYICDDHMPR
  VVTHTNATACSCYILNKPVFITMDGAVRRTADLFLPDSFMQEIIGGQARETGDDRPVLTKSAGERFLLYR
  PSTTTNSGLMAPELYVYVDPAFTANTRASGTGIAVVGRYRDDFIIFALEHFFLRALTGSAPADIARCVVH
  SLAQVLALHPGAFRSVRVAVEGNSSQDSAVAIATHVHTEMHRILASAGANGPGPELLFYHCEPPGGAVLY
  PFFLLNKQKTPAFEYFIKKFNSGGVMASQELVSVTVRLQTDPVEYLSEQLNNLIETVSPNTDVRMYSGKR
  NGAADDLMVAVIMAIYLAAPTGIPPAFFPITRTS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001798
• ORF Size: 2202 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001798.1, NP_044484
• RefSeq ORF: 2202 bp
• Locus ID: 1487298
• MW: 81.0 kDa