UL30 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100818

Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase catalytic subunit [Human herpesvirus 1], codon optimized for human cell expression, NP_044632. Note: ORF is codon optimized

Product Images

Circular map for VC100818

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >VC100818 representing NP_044632, codon optimized.
  Due to the complexity of NP_044632, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTTAGCGGAGGTGGGGGACCTCTGTCTCCTGGAGGAAAAAGCGCGGCACGAGCCGCATCTGGCTTT
  TTCGCACCTGCGGGTCCACGCGGTGCGTCACGCGGCCCACCACCCTGTCTTCGCCAGAACTTCTATAAC
  CCCTACCTGGCACCTGTCGGCACTCAACAGAAGCCTACTGGTCCCACCCAAAGGCACACCTACTACTCT
  GAGTGCGACGAGTTCCGATTCATTGCCCCGCGAGTGTTGGATGAGGATGCCCCCCCAGAGAAGCGGGCC
  GGGGTGCATGATGGACATCTGAAGAGAGCACCGAAGGTTTACTGTGGAGGGGATGAAAGGGATGTGCTT
  CGGGTGGGCTCAGGTGGATTTTGGCCCCGAAGAAGTAGACTCTGGGGTGGGGTGGATCATGCTCCTGCC
  GGTTTCAATCCCACTGTCACTGTGTTCCACGTGTATGACATTCTTGAAAACGTCGAACACGCTTACGGT
  ATGCGAGCCGCTCAGTTTCACGCGAGATTTATGGACGCCATCACACCAACTGGCACCGTGATCACTCTC
  CTGGGCCTTACTCCAGAAGGCCACCGCGTTGCTGTGCATGTGTACGGCACCAGGCAGTACTTCTATATG
  AACAAAGAAGAGGTGGATAGACACCTTCAGTGTAGAGCACCTCGCGACCTGTGTGAAAGAATGGCAGCA
  GCTCTCCGAGAATCACCAGGCGCTAGCTTTCGGGGTATCAGCGCAGACCATTTTGAGGCCGAAGTGGTA
  GAGCGCACCGACGTCTACTACTATGAAACTCGACCCGCCCTGTTCTACCGCGTATATGTGCGCTCTGGA
  AGAGTTCTCTCTTACCTCTGCGATAACTTTTGTCCTGCCATTAAGAAGTATGAAGGGGGTGTCGACGCT
  ACCACACGCTTCATCTTGGATAACCCGGGGTTCGTTACATTCGGTTGGTATCGACTGAAGCCAGGTCGG
  AACAATACCCTCGCACAACCAGCGGCCCCGATGGCCTTTGGGACATCCTCCGACGTGGAGTTCAACTGC
  ACTGCTGATAACCTGGCTATAGAAGGCGGCATGAGCGACTTGCCAGCATACAAGTTGATGTGCTTTGAT
  ATCGAGTGCAAAGCAGGGGGAGAGGACGAACTGGCCTTCCCTGTCGCGGGGCATCCTGAAGATCTGGTG
  ATCCAAATTTCATGCCTGCTGTATGATCTCTCCACCACTGCCCTTGAGCATGTGCTTCTCTTTTCTTTG
  GGCAGTTGCGACCTGCCCGAGTCTCACCTGAACGAGCTGGCCGCGCGCGGCCTGCCCACCCCAGTGGTC
  CTGGAATTCGACAGTGAGTTTGAGATGCTCCTGGCTTTTATGACCCTCGTGAAGCAGTATGGGCCTGAG
  TTTGTAACAGGATATAACATAATTAATTTTGACTGGCCCTTTTTGCTCGCAAAACTGACCGACATCTAC
  AAAGTACCGCTCGACGGCTATGGGCGCATGAATGGCCGCGGCGTCTTCCGCGTGTGGGACATTGGCCAA
  TCCCATTTCCAGAAAAGATCTAAGATTAAGGTTAACGGAATGGTGAATATCGATATGTACGGCATAATC
  ACAGACAAGATCAAGTTGAGCAGTTATAAACTTAACGCAGTGGCTGAAGCCGTTCTGAAGGATAAAAAG
  AAGGATCTGAGTTACAGGGATATCCCGGCTTACTATGCCGCTGGCCCTGCTCAGCGCGGCGTCATTGGG
  GAGTACTGTATCCAGGACAGTCTTCTGGTTGGACAGCTGTTTTTCAAATTTTTGCCCCATCTGGAACTG
  TCCGCCGTGGCACGACTGGCCGGAATCAACATAACCCGAACCATTTATGATGGGCAGCAGATCCGGGTA
  TTCACATGTCTGTTGAGACTGGCTGACCAGAAAGGTTTCATCCTTCCCGATACTCAGGGAAGATTCAGA
  GGCGCCGGGGGCGAGGCCCCTAAGCGACCTGCGGCAGCAAGAGAGGATGAAGAACGGCCAGAAGAAGAG
  GGAGAGGATGAAGACGAAAGGGAAGAGGGCGGCGGTGAAAGAGAACCTGAAGGTGCCAGAGAGACGGCC
  GGGCGACATGTTGGGTATCAGGGCGCTAGGGTCCTCGATCCCACATCCGGATTCCATGTTAATCCTGTG
  GTGGTCTTCGACTTTGCATCTCTCTACCCATCAATAATCCAGGCGCACAACCTTTGCTTCTCTACACTC
  TCCCTGAGGGCAGACGCCGTGGCTCACCTGGAAGCCGGGAAAGACTACCTCGAAATTGAGGTGGGGGGT
  CGCCGACTGTTCTTTGTGAAAGCTCACGTGCGCGAAAGCCTCCTTAGTATCTTGCTGAGAGATTGGCTC
  GCTATGAGAAAACAAATCCGGAGCAGGATTCCCCAGAGTAGCCCAGAAGAAGCCGTTCTTCTTGACAAA
  CAGCAGGCTGCCATTAAGGTTGTGTGTAACTCTGTGTATGGCTTTACTGGGGTCCAACACGGGCTGCTC
  CCCTGCTTGCACGTGGCTGCTACTGTGACAACGATTGGACGCGAGATGCTGCTTGCCACACGCGAATAC
  GTACATGCTCGGTGGGCAGCGTTCGAGCAGCTCCTGGCCGACTTTCCAGAGGCAGCAGATATGAGAGCA
  CCCGGGCCGTACTCTATGAGAATTATCTACGGTGACACAGACAGCATTTTCGTGCTTTGCCGGGGACTC
  ACGGCCGCAGGCTTGACCGCTGTTGGGGATAAGATGGCCTCACACATAAGTCGCGCTCTGTTCCTGCCC
  CCAATCAAGCTGGAGTGCGAAAAAACCTTTACCAAACTGTTGCTTATAGCCAAGAAGAAGTACATTGGG
  GTTATCTATGGCGGGAAGATGCTTATCAAAGGAGTCGACCTCGTGCGAAAGAATAACTGCGCCTTCATC
  AACAGAACCTCCAGGGCACTTGTTGATCTCTTGTTCTATGACGACACCGTTAGCGGAGCAGCTGCAGCC
  CTCGCTGAGCGGCCTGCGGAGGAATGGTTGGCCAGACCACTGCCCGAAGGACTCCAGGCATTCGGTGCC
  GTGCTGGTCGATGCTCACAGGCGAATTACTGATCCAGAGCGCGACATTCAGGACTTCGTACTGACCGCC
  GAACTCTCACGGCATCCCAGAGCGTACACCAACAAACGCCTCGCTCATTTGACAGTGTATTACAAGCTT
  ATGGCTCGGAGAGCTCAAGTCCCATCCATCAAAGATAGAATTCCTTATGTCATTGTCGCGCAGACTCGA
  GAGGTTGAGGAAACCGTGGCAAGACTGGCCGCCCTGAGAGAACTGGATGCCGCTGCACCTGGTGATGAG
  CCTGCCCCCCCTGCAGCCCTGCCATCACCAGCTAAAAGGCCCAGAGAGACCCCGAGTCCAGCTGATCCA
  CCAGGTGGGGCCAGTAAACCTCGAAAACTCCTGGTAAGCGAGCTGGCAGAGGATCCAGCATATGCTATT
  GCCCACGGTGTTGCACTTAACACCGACTATTACTTTAGCCATCTCCTGGGGGCTGCTTGTGTTACATTC
  AAAGCACTGTTCGGGAACAACGCCAAGATCACCGAGTCCCTCTTGAAGAGATTTATACCAGAGGTCTGG
  CATCCCCCCGATGATGTCGCCGCTCGACTGCGCACAGCAGGGTTCGGCGCCGTCGGAGCAGGAGCAACC
  GCGGAGGAAACTCGACGAATGCTTCATAGGGCCTTTGATACGTTGGCC
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >VC100818 representing NP_044632
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MFSGGGGPLSPGGKSAARAASGFFAPAGPRGASRGPPPCLRQNFYNPYLAPVGTQQKPTGPTQRHTYYS
  ECDEFRFIAPRVLDEDAPPEKRAGVHDGHLKRAPKVYCGGDERDVLRVGSGGFWPRRSRLWGGVDHAPA
  GFNPTVTVFHVYDILENVEHAYGMRAAQFHARFMDAITPTGTVITLLGLTPEGHRVAVHVYGTRQYFYM
  NKEEVDRHLQCRAPRDLCERMAAALRESPGASFRGISADHFEAEVVERTDVYYYETRPALFYRVYVRSG
  RVLSYLCDNFCPAIKKYEGGVDATTRFILDNPGFVTFGWYRLKPGRNNTLAQPAAPMAFGTSSDVEFNC
  TADNLAIEGGMSDLPAYKLMCFDIECKAGGEDELAFPVAGHPEDLVIQISCLLYDLSTTALEHVLLFSL
  GSCDLPESHLNELAARGLPTPVVLEFDSEFEMLLAFMTLVKQYGPEFVTGYNIINFDWPFLLAKLTDIY
  KVPLDGYGRMNGRGVFRVWDIGQSHFQKRSKIKVNGMVNIDMYGIITDKIKLSSYKLNAVAEAVLKDKK
  KDLSYRDIPAYYAAGPAQRGVIGEYCIQDSLLVGQLFFKFLPHLELSAVARLAGINITRTIYDGQQIRV
  FTCLLRLADQKGFILPDTQGRFRGAGGEAPKRPAAAREDEERPEEEGEDEDEREEGGGEREPEGARETA
  GRHVGYQGARVLDPTSGFHVNPVVVFDFASLYPSIIQAHNLCFSTLSLRADAVAHLEAGKDYLEIEVGG
  RRLFFVKAHVRESLLSILLRDWLAMRKQIRSRIPQSSPEEAVLLDKQQAAIKVVCNSVYGFTGVQHGLL
  PCLHVAATVTTIGREMLLATREYVHARWAAFEQLLADFPEAADMRAPGPYSMRIIYGDTDSIFVLCRGL
  TAAGLTAVGDKMASHISRALFLPPIKLECEKTFTKLLLIAKKKYIGVIYGGKMLIKGVDLVRKNNCAFI
  NRTSRALVDLLFYDDTVSGAAAALAERPAEEWLARPLPEGLQAFGAVLVDAHRRITDPERDIQDFVLTA
  ELSRHPRAYTNKRLAHLTVYYKLMARRAQVPSIKDRIPYVIVAQTREVEETVARLAALRELDAAAPGDE
  PAPPAALPSPAKRPRETPSPADPPGGASKPRKLLVSELAEDPAYAIAHGVALNTDYYFSHLLGAACVTF
  KALFGNNAKITESLLKRFIPEVWHPPDDVAARLRTAGFGAVGAGATAEETRRMLHRAFDTLA
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NC_001806
• ORF Size: 3705 bp
• OTI Disclaimer: This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NP_044632
• RefSeq ORF: 3705 bp
• Locus ID: 2703462
• UniProt ID: P04413
• MW: 136.4 kDa