E2B (NC_001454) Virus Tagged ORF Clone

CAT#: VC100505

Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase [Human adenovirus F], codon optimized for human cell expression, NP_040853

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100505 represents NCBI reference of NP_040853 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGGTCCCTTCTCCGAGGGCAGGCGGGTTCCTGCCAGCAGAAACCCATAGCGGGCCGCAACCCC
  CACGAAGGAGAGTTAGGCAAAGTACCGCGGGGGCAGCCCCAACGGCAACTCGGGCTCCTCGACGAAGAGC
  CGCCACCGCTTCTCCTGGCGAACCTCCAAGTACTACGGCGTCTGGAAGACCTCCAGCCGCCAACAACGTG
  AGCCTCACACCCAACTCACGGCTCAGGGGTACCATTGTGGCCCCACGGGGTCAAGGTCTGTTGTATGCTA
  TCGACACCGCTACTAATTCCCCCATGGAGATCAAGTTCCATCGCCGACTTGCATCCGCTCTGACGCGATT
  GTTGCAAGTGAACCTGCGGAGCGTGCCTGCCGATCTGAACGAGGCATTTCTCGATTCACTGGATTCAAGC
  CAGATCCGCACACTTGCCCTGAAGCTTAAGGTGCCAAGAGTGGAGGTTTGGACATGTGGAAGTCGGGGGG
  TAGTGGTGCCCTCCATTATCCACCCACAACAGGAACGCGCAGGCGCTGAGGAGGGTGACGAGGGCGAACG
  GCAGGACACAGAAGACTTTCTCAACTTCCCTCTCAGATTCCTGGTGAGGGGCAGGCAGGTTCATCTGATC
  CAGGAGATGCAATCAGTGCAGCGGTGCGAGTACTGCGCCCGCTTTTATAAGTATCAGCATGAGTGTACCG
  TACGCCGGCGGGATTTTTATTTTCACCACATTAATGCCCATAGCTCTGGATGGTGGCAGAAAATTAACTT
  TTTTCCGATCGGATCTCATCCTAGAGTCGAGCGCCTCTTCGTGACCTACGATGTGGAAACGTATACATGG
  ATGGGTGCCTTCGGAAAGCAGCTGGTCCCGTTCATGCTGGTAATGCACCTGTCAGGCGAGGAGGCTCTTG
  TTAAGGAAGCATGTAGGCTGGCCTGTGAGCTGCAGTGGGACACTTGGGGCAATGATGAACGAACATTCTA
  TGTAGTGACCCCGGAGAAGCTGGCCGTTGGGAAAAAGTTTCGAGAATATCGGAACCGGCTGCAGGCCCAT
  TTTGCCCTGCAATTGTGGCGGGGTTTTCTCGCGGCTAATCCACAGCTGGCAGAGTGGGCATGCTTGGAAA
  TGGGCCTGTTTAGCCCTGATTATCTCACGTACGAAGAACTTCAGAAGGCCCCCAAGCTCCAAGGGCGGCC
  CAGGTTTCTCGAATTGTACATCGTGGGACACAACATTAACGGGTTCGACGAGATCGTGCTCGCGGCACAG
  GTGATCAACAACAGGAGCGACGTACCTGGGCCTTTTAAGATCACACGAAATTTTATGCCTAGAGCTGGAA
  AAATTCTGTTCAATGACATCACTTTTGCACTGCCAAACCCATCATCTAAAAAGCGGACAGACTACCGCCT
  GTGGGAGCAGGGAGCTTGCGACGATTCAGACTTTAAGTACCAGTTTCTCAAAGTTATGGTCCGCGACACC
  TTTGCATTGACACATACTTCACTGAGAAAGGCCGCACAGGCATATACTTTGCCTGTCGAGAAGGGATGCT
  GTCCCTATAAGGCGGTGAACGAATTCTACATGCTGGGCTCTTATCGAGCCGATGAACGGGGTTTTCCCGC
  TGAGGACTACTGGAAGGACCGGGAGGAGTACCTGCTGAACAGAGAATTGTGGGAGAAGAAGCAGTGTCCA
  CACTATGACCTGGTACGGGAAACGCTGGATTATTGCGCGCTGGATGTCTTGGTGACAGCCGCATTGGTTC
  AAAAGTTGCGCGAATCATACGCCCAGTTCATCAGGGATGCCGTCGGCTTGCCCGAGGCCTCATTCAACGT
  ATTTCAGCGGCCCACGATTTCTAGCAACAGCCACGCTATATTCCGACAAATCCTTTATAGAACAGTGAAG
  CCACAGAGAAGCGACTTGGGGGGCTCTCTGCTTGCTCCATCACATGAAATGTATGACTATGTGCGCGCCA
  GCATCCGGGGTGGTAGATGCTACCCCACCTACATTGGCGTCCTGAGGGAGCCGCTTTACGTTTACGACAT
  TTGTGGCATGTACGCTTCTGCGCTGACACACCCTATGCCATGGGGGTTCCCCTTGAACCCATACGAGAGG
  GCACTGGCTGTTCGGGATTGGGAACACGCACTGCTGCAGGTGGGGACCCCCATCGACTATTTTAACCGGA
  CTCTGCTCCCAGGAATCTTCACGATCGACGCCGATCCTCCTCCCGAGAACCTGTTGGACGTGCTGCCCCC
  ACTGTGCTCCAGGAAGGGTGGGCGGCTGTGTTGGACCAATGAGCCCCTTCGCGGTGAGGTCGTTACAAGC
  GTCGATCTGATCACACTGCACAATCGAGGATGGCATGTGCGCCTTCTGCCCGATGAACGGGCCACTGTGT
  TTCCCGAATGGCGCTGTGTGGCCAAAGAGTACGTGCACCTGAATATTACAGCTAAAGAAAGGGCCGACAG
  GGAGAAGAATCAGACTCTGCGCAGTATCGCCAAGCTCTTGTCCAACGCCCTGTACGGGAGCTTCGCAACC
  AAGCTCGATAATAAGAAGATAGTATTTTCTGACCAGATGGATAGCGCAACAATTAAGAGCATCGCCGCCG
  GGCAGATCAATATAAAGTCTACATCTTTTGTGGAAACCGACACCCTCAGTGCAGAAGTCATGCCAACATT
  TCAGCGCGCTTACTCTCCGGAGCAGCTGGCTGTGGTCCATAGCGATGCTGAAGAATCAGATGAAGAGCCC
  GGCCACGCCCCATTTTACACTCCCACCCACAAGCCCAACGATCATGTGACCTACACGTACAAACCTATCA
  CATTTATGGACGCCGAAGAGGACGACCTTTGCCTGCACACTCTCGAGAAGGTGGACCCGCTGGTTGAGAA
  TAATCGCTACCCCTCCCAGATAGCCTCCTTCGTCCTCGCGTGGACCCGGGCATTCGTGTCCGAATGGTCA
  GAAATTCTGTACGCAGAGGATCGCGGGACCCCTTTGGAACAGCGGACTCTCAAATCCGTCTACGGCGACA
  CAGACTCTCTGTTTGTAACTGAAGCGGGCTATAGACTGATGGAGACAAGAGGCAAGAAGCGAATCAAGAA
  ACACGGCGGTAACCTGGTTTTTGATCCTAAGCATCCCGAGCTTGCTTGGCTGGTGGAATGCGAAACCGTC
  TGTGCTCAATGCGGGGCGGACGCTTATAGCCCAGAGTCCGTTTTCCTTGCGCCAAAGCTGTATGCACTGA
  AATGCCTCCGGTGTCCGAGCTGTCAGCAAATTTCCAAAGGCAAGCTCAGAGCGAAAGGGCACGCTGCCGA
  GACTCTGAATTATGATCTCATGCTTAAATGCTATCTCGCGGACTTCCAGGGAGAGGACGCCAGATTTCAC
  ACAAGCCGGATGTCTTTGAAAAGGACCCTGGCCAGCGCACAGCCTGGGGCAAGACCTTTTACAGTAACCG
  AAACGAATCTCACAAGGACATTGAGACCTTGGAAAGATATCACCCTGGCCCCACTGGATGCCCATCGCCT
  GGTGCCATATTCTCAGAGTCGGCCCAATCCAAGGAACCAGGAAGTTTGCTGGATCGAAATGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100505 representing NP_040853
  Red=Cloning sites Green=Tags
  
  MALVPSPRAGGFLPAETHSGPQPPRRRVRQSTAGAAPTATRAPRRRAATASPGEPPSTTASGRPPAANNV
  SLTPNSRLRGTIVAPRGQGLLYAIDTATNSPMEIKFHRRLASALTRLLQVNLRSVPADLNEAFLDSLDSS
  QIRTLALKLKVPRVEVWTCGSRGVVVPSIIHPQQERAGAEEGDEGERQDTEDFLNFPLRFLVRGRQVHLI
  QEMQSVQRCEYCARFYKYQHECTVRRRDFYFHHINAHSSGWWQKINFFPIGSHPRVERLFVTYDVETYTW
  MGAFGKQLVPFMLVMHLSGEEALVKEACRLACELQWDTWGNDERTFYVVTPEKLAVGKKFREYRNRLQAH
  FALQLWRGFLAANPQLAEWACLEMGLFSPDYLTYEELQKAPKLQGRPRFLELYIVGHNINGFDEIVLAAQ
  VINNRSDVPGPFKITRNFMPRAGKILFNDITFALPNPSSKKRTDYRLWEQGACDDSDFKYQFLKVMVRDT
  FALTHTSLRKAAQAYTLPVEKGCCPYKAVNEFYMLGSYRADERGFPAEDYWKDREEYLLNRELWEKKQCP
  HYDLVRETLDYCALDVLVTAALVQKLRESYAQFIRDAVGLPEASFNVFQRPTISSNSHAIFRQILYRTVK
  PQRSDLGGSLLAPSHEMYDYVRASIRGGRCYPTYIGVLREPLYVYDICGMYASALTHPMPWGFPLNPYER
  ALAVRDWEHALLQVGTPIDYFNRTLLPGIFTIDADPPPENLLDVLPPLCSRKGGRLCWTNEPLRGEVVTS
  VDLITLHNRGWHVRLLPDERATVFPEWRCVAKEYVHLNITAKERADREKNQTLRSIAKLLSNALYGSFAT
  KLDNKKIVFSDQMDSATIKSIAAGQINIKSTSFVETDTLSAEVMPTFQRAYSPEQLAVVHSDAEESDEEP
  GHAPFYTPTHKPNDHVTYTYKPITFMDAEEDDLCLHTLEKVDPLVENNRYPSQIASFVLAWTRAFVSEWS
  EILYAEDRGTPLEQRTLKSVYGDTDSLFVTEAGYRLMETRGKKRIKKHGGNLVFDPKHPELAWLVECETV
  CAQCGADAYSPESVFLAPKLYALKCLRCPSCQQISKGKLRAKGHAAETLNYDLMLKCYLADFQGEDARFH
  TSRMSLKRTLASAQPGARPFTVTETNLTRTLRPWKDITLAPLDAHRLVPYSQSRPNPRNQEVCWIEMP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001454
• ORF Size: 3564 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001454.1, NP_040853
• RefSeq ORF: 3564 bp
• Locus ID: 2715933
• MW: 135.3 kDa