L1 (NC_011202) Virus Tagged ORF Clone

CAT#: VC100311

Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein 52K [Human adenovirus B], codon optimized for human cell expression, YP_002213805

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100311 represents NCBI reference of YP_002213805 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCCGGTGCTGAGACAGATGAGACCCCAGCAACAACCACCTAGTCAGCAGCAGCAACAGCAGTCCC
  AGAAGGCAGTTCCAGCGACTACTGCCACCGCAGCAGTCTCTGGAGCCGGACAGCCGGCATACGATCTGGA
  TCTCGAGGAAGGAGAGGGACTGGCCAGGCTCGGCGCACCCTCCCCAGAGAGACATCCGAGGGTGCAGCTC
  AAGAAGGACTCTCGGGAAGCTTACGTTCCTCAGCAGAATCTGTTCCGGGATAGAAGTGGCGAGGAGCCTG
  AGGAGATGAGAGCCAGTCGGTTTAACGCCGGAAGGGAGCTTCGGCACGGGCTCGATAGAAGAAGGGTCCT
  GAGAGACGAAGATTTTGAGGTGGATGAGATGACCGGCATCAGCCCTGCGCGCGCCCACGTAGCTGCCGCC
  AACCTCGTTAGCGCCTATGAACAGACAGTGAAAGAGGAGAGGAATTTTCAGAAAAGTTTCAACAATCACG
  TGCGGACACTGATCGCCCGAGAAGAAGTGACTCTCGGCCTGATGCACCTTTGGGACTTGATGGAAGCTAT
  TATACAGAACCCAACGTCCAAGCCTTTGACAGCCCAGCTTTTCCTTGTAGTTCAGCATTCACGCGATAAC
  GAGGCTTTCCGCGAGGCTCTCCTCAATATTACAGAACCGGAGGGACGGTGGCTGTACGATCTCATCAATA
  TCCTGCAATCTATCATCGTCCAGGAACGATCACTGGGCCTCGCAGAGAAGGTCGCAGCAATCAACTATTC
  AGTATTGAGCCTGGGTAAATACTATGCACGGAAGATATATAAAACTCCATACGTGCCCATTGACAAAGAA
  GTGAAGATAGACGGATTTTACATGCGCATGACTCTGAAGGTGCTTACCCTGAGCGATGATCTGGGGGTGT
  ACCGGAATGACAGAATGCATAGAGCTGTGTCCGCCAGCCGCCGAAGAGAGCTCTCTGATCGAGAGTTGAT
  GCACTCCCTGCAAAGGGCTCTGACAGGCGCCGGAACCGAGGGCGAGAATTACTTTGACATGGGCGCGGAT
  CTGCAGTGGCAGCCGTCAAGGCGCGCCCTGAGCGCAGCAACAGCCGGTTGCGAGTTGCCCTACATTGAGG
  AGGCAGATGAAGGGGAGGAGGAAGAGGGCGAGTATCTGGAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100311 representing YP_002213805
  Red=Cloning sites Green=Tags
  
  MHPVLRQMRPQQQPPSQQQQQQSQKAVPATTATAAVSGAGQPAYDLDLEEGEGLARLGAPSPERHPRVQL
  KKDSREAYVPQQNLFRDRSGEEPEEMRASRFNAGRELRHGLDRRRVLRDEDFEVDEMTGISPARAHVAAA
  NLVSAYEQTVKEERNFQKSFNNHVRTLIAREEVTLGLMHLWDLMEAIIQNPTSKPLTAQLFLVVQHSRDN
  EAFREALLNITEPEGRWLYDLINILQSIIVQERSLGLAEKVAAINYSVLSLGKYYARKIYKTPYVPIDKE
  VKIDGFYMRMTLKVLTLSDDLGVYRNDRMHRAVSASRRRELSDRELMHSLQRALTGAGTEGENYFDMGAD
  LQWQPSRRALSAATAGCELPYIEEADEGEEEEGEYLED
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_011202
• ORF Size: 1164 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_011202.1, YP_002213805
• RefSeq ORF: 1164 bp
• Locus ID: 6870507
• MW: 44.2 kDa