Calpain 2 (CAPN2) (NM_001146068) Human Untagged Clone

CAT#: SC327142

CAPN2 (untagged)-Human calpain 2 (m/II) large subunit (CAPN2) transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CAPN2
• Synonyms: CANP2; CANPL2; CANPml; mCANP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001146068, the custom clone sequence may differ by one or more nucleotides
  ATGGAGATCTGCGCTGACCCCCAGTTTATCATTGGAGGAGCCACCCGCACAGACATCTGC
  CAAGGAGCCCTGGGTGACTGCTGGCTGCTGGCAGCCATTGCCTCCCTCACCTTGAATGAA
  GAAATCCTGGCTCGAGTCGTCCCCCTAAACCAGAGCTTCCAGGAAAACTATGCAGGGATC
  TTTCACTTCCAGTTCTGGCAATACGGCGAGTGGGTGGAGGTGGTGGTGGATGACAGGCTG
  CCCACCAAGGACGGGGAGCTGCTCTTTGTGCATTCAGCCGAAGGGAGCGAGTTCTGGAGC
  GCCCTGCTGGAGAAGGCATACGCCAAGATCAACGGATGCTATGAAGCGCTATCAGGGGGT
  GCCACCACTGAGGGCTTCGAAGACTTCACCGGAGGCATTGCTGAGTGGTATGAGTTGAAG
  AAGCCCCCTCCCAACCTGTTCAAGATCATCCAGAAAGCTCTGCAAAAAGGCTCTCTCCTT
  GGCTGCTCCATCGACATCACCAGCGCCGCGGACTCGGAGGCCATCACGTTTCAGAAGCTG
  GTGAAGGGGCACGCGTACTCGGTCACCGGAGCCGAGGAGGTTGAAAGTAACGGAAGCCTA
  CAGAAACTGATCCGCATCCGAAATCCCTGGGGAGAAGTGGAGTGGACAGGGCGGTGGAAT
  GACAACTGCCCAAGCTGGAACACTATAGACCCAGAGGAGAGGGAAAGGCTGACCAGACGG
  CATGAAGATGGAGAATTCTGGATGTCTTTCAGTGACTTCCTGAGGCACTATTCCCGCCTG
  GAGATCTGTAACCTGACCCCAGACACTCTCACCAGCGATACCTACAAGAAGTGGAAACTC
  ACCAAAATGGATGGGAACTGGAGGCGGGGCTCCACCGCGGGAGGTTGCAGGAACTACCCG
  AACACATTCTGGATGAACCCTCAGTACCTGATCAAGCTGGAGGAGGAGGATGAGGACGAG
  GAGGATGGGGAGAGCGGCTGCACCTTCCTGGTGGGGCTCATTCAGAAGCACCGACGGCGG
  CAGAGGAAGATGGGCGAGGACATGCACACCATCGGCTTTGGCATCTATGAGGTTCCAGAG
  GAGTTAAGTGGGCAGACCAACATCCACCTCAGCAAAAACTTCTTCCTGACGAATCGCGCC
  AGGGAGCGCTCAGACACCTTCATCAACCTCCGGGAGGTGCTCAACCGCTTCAAGCTGCCG
  CCAGGAGAGTACATTCTCGTGCCTTCCACCTTCGAACCCAACAAGGATGGGGATTTCTGC
  ATCCGGGTCTTTTCTGAAAAGAAAGCTGACTACCAAGCTGTCGATGATGAAATCGAGGCC
  AATCTTGAAGAGTTCGACATCAGCGAGGATGACATTGATGATGGATTCAGGAGACTGTTT
  GCCCAGTTGGCAGGAGAGGATGCGGAGATCTCTGCCTTTGAGCTGCAGACCATCCTGAGA
  AGGGTTCTAGCAAAGCGCCAAGATATCAAGTCAGATGGCTTCAGCATCGAGACATGCAAA
  ATTATGGTTGACATGCTAGATTCGGACGGGAGTGGCAAGCTGGGGCTGAAGGAGTTCTAC
  ATTCTCTGGACGAAGATTCAAAAATACCAAAAAATTTACCGAGAAATCGACGTTGACAGG
  TCTGGTACCATGAATTCCTATGAAATGCGGAAGGCATTAGAAGAAGCAGGTTTCAAGATG
  CCCTGTCAACTCCACCAAGTCATCGTTGCTCGGTTTGCAGATGACCAGCTCATCATCGAT
  TTTGATAATTTTGTTCGGTGTTTGGTTCGGCTGGAAACGCTATTCAAGATATTTAAGCAG
  CTGGATCCCGAGAATACTGGAACAATAGAGCTCGACCTTATCTCTTGGCTCTGTTTCTCA
  GTACTT
  
• Restriction Sites: Please inquire
• ACCN: NM_001146068
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146068.1, NP_001139540.1
• RefSeq Size: 3270 bp
• RefSeq ORF: 1869 bp
• Locus ID: 824
• UniProt ID: P17655
• Cytogenetics: 1q41
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alzheimer's disease, Apoptosis, Focal adhesion
• Gene Summary: The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1.