DISC1 (NM_001164545) Human Untagged Clone

CAT#: SC327104

DISC1 (untagged)-Human disrupted in schizophrenia 1 (DISC1) transcript variant h

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DISC1
• Synonyms: C1orf136; SCZD9
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001164545, the custom clone sequence may differ by one or more nucleotides
  ATGCCAGGCGGGGGTCCTCAGGGCGCCCCAGCCGCCGCCGGCGGCGGCGGCGTGAGCCAC
  CGCGCAGGCAGCCGGGATTGCTTACCACCTGCAGCGTGCTTTCGGAGGCGGCGGCTGGCA
  CGGAGGCCGGGCTACATGAGAAGCTCGACAGGGCCTGGGATCGGGTTCCTTTCCCCAGCA
  GTGGGCACACTGTTCCGGTTCCCAGGAGGGGTGTCTGGCGAGGAGTCCCACCACTCGGAG
  TCCAGGGCCAGACAGTGTGGCCTTGACTCGAGAGGCCTCTTGGTCCGGAGCCCTGTTTCC
  AAGAGTGCAGCAGCCCCTACTGTGACCTCTGTGAGAGGAACCTCGGCGCACTTTGGGATT
  CAGCTCAGAGGTGGCACCAGATTGCCTGACAGGCTTAGCTGGCCGTGTGGCCCTGGGAGT
  GCTGGGTGGCAGCAAGAGTTTGCAGCCATGGATAGTTCTGAGACCCTGGACGCCAGCTGG
  GAGGCAGCCTGCAGCGATGGAGCAAGGCGTGTCCGGGCAGCAGGCTCTCTGCCATCAGCA
  GAGTTGAGTAGCAACAGCTGCAGCCCTGGCTGTGGCCCTGAGGTCCCCCCAACCCCTCCT
  GGCTCTCACAGTGCCTTTACCTCAAGCTTTAGCTTTATTCGGCTCTCGCTTGGCTCTGCC
  GGGGAACGTGGAGAAGCAGAAGGCTGCCCACCATCCAGAGAGGCTGAGTCCCATTGCCAG
  AGCCCCCAGGAGATGGGAGCCAAAGCTGCCAGCTTGGACGGGCCTCACGAGGACCCGCGA
  TGTCTCTCTCGGCCCTTCAGTCTCTTGGCTACACGGGTCTCTGCAGACTTGGCCCAGGCC
  GCAAGGAACAGCTCCAGGCCAGAGCGTGACATGCATTCTTTACCAGACATGGACCCTGGC
  TCCTCCAGTTCTCTGGATCCCTCACTGGCTGGCTGTGGTGGTGATGGGAGCAGCGGCTCA
  GGGGATGCCCACTCTTGGGACACCCTGCTCAGGAAATGGGAGCCAGTGCTGCGGGACTGC
  CTGCTGAGAAACCGGAGGCAGATGGAGGTAATATCCTTAAGATTAAAACTTCAGAAACTT
  CAGGAAGATGCAGTTGAGAATGATGATTATGATAAAGCTGAGACGTTACAACAAAGATTA
  GAAGACCTGGAACAAGAGAAAATCAGCCTGCACTTTCAACTTCCTTCAAGGCAGCCAGCT
  CTTAGCAGTTTCCTGGGTCACCTGGCAGCACAAGTCCAGGCTGCCTTGCGCCGTGGGGCC
  ACTCAGCAGGCCAGCGGAGATGACACCCACACCCCACTGAGAATGGAGCCGAGGCTGTTG
  GAACCCACTGCTCAGGACAGCTTGCACGTGTCCATCACGAGACGAGACTGGCTTCTTCAG
  GAAAAGCAGCAGCTACAGAAAGAAATCGAAGCTCTCCAAGCAAGGATGTTTGTGCTGGAA
  GCCAAAGATCAACAGCTGAGAAGGGAAATAGAGGAGCAAGAGCAGCAACTCCAGTGGCAG
  GGCTGCGACCTGACCCCACTGGTGGGCCAGCTGTCCCTGGGTCAGCTGCAGGAGGTCAGC
  AAGGCCTTGCAGGACACCCTGGCCTCAGCCGGTCAGATTCCCTTCCATGCAGAGCCACCG
  GAAACCATAAGGAGCCTCCAGGAAAGAATAAAATCCCTCAACTTGTCACTTAAAGAAATC
  ACTACTAAGGAAACCATTTCTGGACGGCTAAAGACCTCACCGAGGAGATTAGATCAT
  
• Restriction Sites: Please inquire
• ACCN: NM_001164545
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164545.1, NP_001158017.1
• RefSeq Size: 2658 bp
• RefSeq ORF: 1740 bp
• Locus ID: 27185
• UniProt ID: Q9NRI5
• Cytogenetics: 1q42.2
• Gene Summary: This gene encodes a protein with multiple coiled coil motifs which is located in the nucleus, cytoplasm and mitochondria. The protein is involved in neurite outgrowth and cortical development through its interaction with other proteins. This gene is disrupted in a t(1;11)(q42.1;q14.3) translocation which segregates with schizophrenia and related psychiatric disorders in a large Scottish family. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (h) lacks an internal exon and multiple 3' exons but has an alternate 3' segment, as compared to variant L. The resulting isoform (h, also known as isoform 35) is much shorter and has a distict C-terminus, as compared to isoform L.