GLB1 (NM_001135602) Human Untagged Clone

CAT#: SC326059

GLB1 (untagged)-Human galactosidase, beta 1 (GLB1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GLB1
• Synonyms: EBP; ELNR1; MPS4B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001135602 edited
  CCCTCGTGCCGAATTCGGCACGAGGGTCAAGTGACGCGAAGCGGCCGGCCTGGGCGCCGA
  CTGCAGAGCCGGGAGGCTGGTGGTCATGCCGGGGTTCCTGGTTCGCATCCTCCCTCTGCT
  GCTGGTTCTGCTGCTTCTGGGCCCTACGCGCGGCTTGCGCAATGCCACCCAGAGGATGTT
  TGAAATTGACTATAGCCGGGACTCCTTCCTCAAGGATGGCCAGCCATTTCGCTACATCTC
  AGGAAGCATTCACTACTCCCGTGTGCCCCGCTTCTACTGGAAGGACCGGCTGCTGAAGAT
  GAAGATGGCTGGGCTGAACGCCATCCAGACATTACCTGGCAGCTGTGGACAAGTGGTTGG
  GAGTCCTTCTGCCCAAGATGAAGCCTCTCCTCTATCAGAATGGAGGGCCAGTTATAACAG
  TGCAGGCAGCAACATCACAGATGCTTTCCTAAGCCAGAGGAAGTGTGAGCCCAAAGGACC
  CTTGATCAATTCTGAATTCTATACTGGCTGGCTAGATCACTGGGGCCAACCTCACTCCAC
  AATCAAGACCGAAGCAGTGGCTTCCTCCCTCTATGATATACTTGCCCGTGGGGCGAGTGT
  GAACTTGTACATGTTTATAGGTGGGACCAATTTTGCCTATTGGAATGGGGCCAACTCACC
  CTATGCAGCACAGCCCACCAGCTACGACTATGATGCCCCACTGAGTGAGGCTGGGGACCT
  CACTGAGAAGTATTTTGCTCTGCGAAACATCATCCAGAAGTTTGAAAAAGTACCAGAAGG
  TCCTATCCCTCCATCTACACCAAAGTTTGCATATGGAAAGGTCACTTTGGAAAAGTTAAA
  GACAGTGGGAGCAGCTCTGGACATTCTGTGTCCCTCTGGGCCCATCAAAAGCCTTTATCC
  CTTGACATTTATCCAGGTGAAACAGCATTATGGGTTTGTGCTGTACCGGACAACACTTCC
  TCAAGATTGCAGCAACCCAGCACCTCTCTCTTCACCCCTCAATGGAGTCCACGATCGAGC
  ATATGTTGCTGTGGATGGGATCCCCCAGGGAGTCCTTGAGCGAAACAATGTGATCACTCT
  GAACATAACAGGGAAAGCTGGAGCCACTCTGGACCTTCTGGTAGAGAACATGGGACGTGT
  GAACTATGGTGCATATATCAACGATTTTAAGGGTTTGGTTTCTAACCTGACTCTCAGTTC
  CAATATCCTCACGGACTGGACGATCTTTCCACTGGACACTGAGGATGCAGTGCGCAGCCA
  CCTGGGGGGCTGGGGACACCGTGACAGTGGCCACCATGATGAAGCCTGGGCCCACAACTC
  ATCCAACTACACGCTCCCGGCCTTTTATATGGGGAACTTCTCCATTCCCAGTGGGATCCC
  AGACTTGCCCCAGGACACCTTTATCCAGTTTCCTGGATGGACCAAGGGCCAGGTCTGGAT
  TAATGGCTTTAACCTTGGCCGCTATTGGCCAGCCCGGGGCCCTCAGTTGACCTTGTTTGT
  GCCCCAGCACATCCTGATGACCTCGGCCCCAAACACCATCACCGTGCTGGAACTGGAGTG
  GGCACCCTGCAGCAGTGATGATCCAGAACTATGTGCTGTGACGTTCGTGGACAGGCCAGT
  TATTGGCTCATCTGTGACCTACGATCATCCCTCCAAACCTGTTGAAAAAAGACTCATGCC
  CCCACCCCCGCAAAAAAACAAAGATTCATGGCTGGACCATGTATGATGATGAAAGCCTGT
  GTCTTTGAGGGATTCTACCCTGAACATACCTCACAGATCCTCCCTGTCATGCCACATTTC
  ACTGATTGGAATGTGGAAATGGAAAAGGAATTTAGGATGTGCATTTTCACCTGAGGTTTC
  CCTGCATCCCTGCAGTGCCAAAGCCCCACCTTCAGGGACCACCTGGAATGTGTGAGGGGC
  TGACAGCACAGTAACGTGCATACATATCTGCAGGGCTGGAATGGAAGCTTTAAAGGTGGT
  AGTGATTTTTATTTTGGAAGAATCATGTTACCTTTTTGTTAAATAAAATTTGTACTCAAA
  AAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001135602
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001135602.1, NP_001129074.1
• RefSeq Size: 2195 bp
• RefSeq ORF: 1641 bp
• Locus ID: 2720
• UniProt ID: P16278
• Cytogenetics: 3p22.3
• Protein Families: Druggable Genome
• Protein Pathways: Galactose metabolism, Glycosaminoglycan degradation, Glycosphingolipid biosynthesis - ganglio series, Lysosome, Metabolic pathways, Other glycan degradation, Sphingolipid metabolism
• Gene Summary: This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (3) lacks three alternate exons in the coding region compared to variant 1. The encoded isoform (c) is shorter than isoform a. This isoform (c) may undergo proteolytic processing similar to isoform a.