NEUROD1 (NM_002500) Human Untagged Clone

CAT#: SC324139

NEUROD1 (untagged)-Human neurogenic differentiation 1 (NEUROD1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEUROD1
• Synonyms: BETA2; BHF-1; bHLHa3; MODY6; NEUROD; T2D
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002500 edited
  GTCCGGAGGCCCCAGGGTTATGAGACTATCACTGCTCAGGACCTACTAACAACAAAGGAA
  ATCGAAACATGACCAAATCGTACAGCGAGAGTGGGCTGATGGGCGAGCCTCAGCCCCAAG
  GTCCTCCAAGCTGGACAGACGAGTGTCTCAGTTCTCAGGACGAGGAGCACGAGGCAGACA
  AGAAGGAGGACGACCTCGAAGCCATGAACGCAGAGGAGGACTCACTGAGGAACGGGGGAG
  AGGAGGAGGACGAAGATGAGGACCTGGAAGAGGAGGAAGAAGAGGAAGAGGAGGATGACG
  ATCAAAAGCCCAAGAGACGCGGCCCCAAAAAGAAGAAGATGACTAAGGCTCGCCTGGAGC
  GTTTTAAATTGAGACGCATGAAGGCTAACGCCCGGGAGCGGAACCGCATGCACGGACTGA
  ACGCGGCGCTAGACAACCTGCGCAAGGTGGTGCCTTGCTATTCTAAGACGCAGAAGCTGT
  CCAAAATCGAGACTCTGCGCTTGGCCAAGAACTACATCTGGGCTCTGTCGGAGATCCTGC
  GCTCAGGCAAAAGCCCAGACCTGGTCTCCTTCGTTCAGACGCTTTGCAAGGGCTTATCCC
  AACCCACCACCAACCTGGTTGCGGGCTGCCTGCAACTCAATCCTCGGACTTTTCTGCCTG
  AGCAGAACCAGGACATGCCCCCCCACCTGCCGACGGCCAGCGCTTCCTTCCCTGTACACC
  CCTACTCCTACCAGTCGCCTGGGCTGCCCAGTCCGCCTTACGGTACCATGGACAGCTCCC
  ATGTCTTCCACGTTAAGCCTCCGCCGCACGCCTACAGCGCAGCGCTGGAGCCCTTCTTTG
  AAAGCCCTCTGACTGATTGCACCAGCCCTTCCTTTGATGGACCCCTCAGCCCGCCGCTCA
  GCATCAATGGCAACTTCTCTTTCAAACACGAACCGTCCGCCGAGTTTGAGAAAAATTATG
  CCTTTACCATGCACTATCCTGCAGCGACACTGGCAGGGGCCCAAAGCCACGGATCAATCT
  TCTCAGGCACCGCTGCCCCTCGCTGCGAGATCCCCATAGACAATATTATGTCCTTCGATA
  GCCATTCACATCATGAGCGAGTCATGAGTGCCCAGCTCAATGCCATATTTCATGATTAGA
  GGCACGCCAGTTTCACCATTTCCGGGAAACGAACCCACTGTGCTTACAGTGACTGTCGTG
  TTTACAAAAGGCAGCCCTTTGGGTACTACTGCTGCAAAGTGCAAATACTCCAAGCTTCAA
  GTGATATATGTATTTATTGTCATTACTGCCTTTGGAAGAAACAGGGGATCAAAGTTCCTG
  TTCACCTTATGTATTATTTTCTATAGCTCTTCTATTTAAAAAATAAAAAAATACAGTAAA
  GTTTAAAAAATACACCACGAATTTGGTGTGGCTGTATTCAGATCGTATTAATTATCTGAT
  CGGGATAACAAAATCACAAGCAATAATTAGGATCTATGCAATTTTTAAACTAGTAATGGG
  CCAATTAAAATATATATAAATATATATTTTTCAACCAGCATTTTACTACTTGTTACCTTT
  CCCATGCTGAATTATTTTGTTGTGATTTTGTACAGAATTTTTAATGACTTTTTATAATGT
  GGATTTCCTATTTTAAAACCATGCAGCTTCATCAATTTTTATACATATCAGAAAAGTAGA
  ATTATATCTAATTTATACAAAATAATTTAACTAATTTAAACCAGCAGAAAAGTGCTTAGA
  AAGTTATTGTGTTGCCTTAGCACTTCTTTCCTCTCCAATTGTAAAAAAAAAAAAAAAA
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_002500
• Insert Size: 1800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: no
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002500.2, NP_002491.2
• RefSeq Size: 2641 bp
• RefSeq ORF: 1071 bp
• Locus ID: 4760
• UniProt ID: Q13562
• Cytogenetics: 2q31.3
• Domains: HLH
• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young
• Gene Summary: This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the protein-coding transcript. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.