RIPK4 (NM_020639) Human Untagged Clone

CAT#: SC323692

RIPK4 (untagged)-Kinase deficient mutant (K51M) of Human receptor-interacting serine-threonine kinase 4 (RIPK4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RIPK4
• Synonyms: ANKK2; ANKRD3; CHANDS; DIK; NKRD3; PKK; PPS2; RIP4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_020639, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGGCGACGGCGGGACCCCATGGGCCCTGGCGCTGCTGCGCACCTTCGACGCGGGCGAGTTCACGG
  GCTGGGAGAAGGTGGGCTCGGGCGGCTTCGGGCAGGTGTACAAGGTGCGCCATGTCCACTGGAAGACCTG
  GCTGGCCATCAAGTGCTCGCCCAGCCTGCACGTCGACGACAGGGAGCGCATGGAGCTTTTGGAAGAAGCC
  AAGAAGATGGAGATGGCCAAGTTTCGCTACATCCTGCCTGTGTATGGCATCTGCCGCGAACCTGTCGGCC
  TGGTCATGGAGTACATGGAGACGGGCTCCCTGGAAAAGCTGCTGGCTTCGGAGCCATTGCCATGGGATCT
  CCGGTTCCGAATCATCCACGAGACGGCGGTGGGCATGAACTTCCTGCACTGCATGGCCCCGCCACTCCTG
  CACCTGGACCTCAAGCCCGCGAACATCCTGCTGGATGCCCACTACCACGTCAAGATTTCTGATTTTGGTC
  TGGCCAAGTGCAACGGGCTGTCCCACTCGCATGACCTCAGCATGGATGGCCTGTTTGGCACAATCGCCTA
  CCTCCCTCCAGAGCGCATCAGGGAGAAGAGCCGGCTCTTCGACACCAAGCACGATGTATACAGCTTTGCG
  ATCGTCATCTGGGGCGTGCTCACACAGAAGAAGCCGTTTGCAGATGAGAAGAACATCCTGCACATCATGG
  TGAAGGTGGTGAAGGGCCACCGCCCCGAGCTGCCGCCCGTGTGCAGAGCCCGGCCGCGCGCCTGCAGCCA
  CCTGATACGCCTCATGCAGCGGTGCTGGCAGGGGGATCCGCGAGTTAGGCCCACCTTCCAAGAAATTACT
  TCTGAAACCGAGGACCTGTGTGAAAAGCCTGATGACGAAGTGAAAGAAACTGCTCATGATCTGGACGTGA
  AAAGCCCCCCGGAGCCCAGGAGCGAGGTGGTGCCTGCGAGGCTCAAGCGGGCCTCTGCCCCCACCTTCGA
  TAACGACTACAGCCTCTCCGAGCTGCTCTCACAGCTGGACTCTGGAGTTTCCCAGGCTGTCGAGGGCCCC
  GAGGAGCTCAGCCGCAGCTCCTCTGAGTCCAAGCTGCCATCGTCCGGCAGTGGGAAGAGGCTCTCGGGGG
  TGTCCTCGGTGGACTCCGCCTTCTCTTCCAGAGGATCACTGTCGCTGTCCTTTGAGCGGGAACCTTCAAC
  CAGCGATCTGGGCACCACAGACGTCCAGAAGAAGAAGCTTGTGGATGCCATCGTGTCCGGGGACACCAGC
  AAACTGATGAAGATCCTGCAGCCGCAGGACGTGGACCTGGCACTGGACAGCGGTGCCAGCCTGCTGCACC
  TGGCGGTGGAGGCCGGGCAAGAGGAGTGCGCCAAGTGGCTGCTGCTCAACAATGCCAACCCCAACCTGAG
  CAACCGTAGGGGCTCCACCCCGTTGCACATGGCCGTGGAGAGGAGGGTGCGGGGTGTCGTGGAGCTCCTG
  CTGGCGCGGAAGATCAGTGTCAACGCCAAGGATGAGGACCAGTGGACAGCCCTCCACTTTGCAGCCCAGA
  ACGGGGACGAGTCTAGCACACGGCTGCTGTTGGAGAAGAACGCCTCGGTCAACGAGGTGGACTTTGAGGG
  CCGGACGCCCATGCACGTGGCCTGCCAGCACGGGCAGGAGAATATCGTGCGCATCCTGCTGCGCCGAGGC
  GTGGACGTGAGCCTGCAGGGCAAGGATGCCTGGCTGCCACTGCACTACGCTGCCTGGCAGGGCCACCTGC
  CCATCGTCAAGCTGCTGGCCAAGCAGCCGGGGGTGAGTGTGAACGCCCAGACGCTGGATGGGAGGACGCC
  ATTGCACCTGGCCGCACAGCGCGGGCACTACCGCGTGGCCCGCATCCTCATCGACCTGTGCTCCGACGTC
  AACGTCTGCAGCCTGCTGGCACAGACACCCCTGCACGTGGCCGCGGAGACGGGGCACACGAGCACTGCCA
  GGCTGCTCCTGCATCGGGGCGCTGGCAAGGAGGCCATGACCTCAGACGGCTACACCGCTCTGCACCTGGC
  TGCCCGCAACGGACACCTGGCCACTGTCAAGCTGCTTGTCGAGGAGAAGGCCGATGTGCTGGCCCGGGGA
  CCCCTGAACCAGACGGCGCTGCACCTGGCTGCCGCCCACGGGCACTCGGAGGTGGTGGAGGAGTTGGTCA
  GCGCCGATGTCATTGACCTGTTCGACGAGCAGGGGCTCAGCGCGCTGCACCTGGCCGCCCAGGGCCGGCA
  CGCACAGACGGTGGAGACTCTGCTCAGGCATGGGGCCCACATCAACCTGCAGAGCCTCAAGTTCCAGGGC
  GGCCATGGCCCCGCCGCCACGCTCCTGCGGCGAAGCAAGACCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_020639 unedited
  CCCGCCGTTCAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCGACGGCGGGACCC
  CATGGGCCCTGGCGCTGCTGCGCACCTTCGACGCGGGCGAGTTCACGGGCTGGGAGAAGGTGGGCTCGGG
  CGGCTTCGGGCAGGTGTACAAGGTGCGCCATGTCCACTGGAAGACCTGGCTGGCCATCATGTGCTCGCCC
  AGCCTGCACGTCGACGACAGGGAGCGCATGGAGCTTTTTGGAAGAAGCCAAGAAAGATGGGAGATGGGCC
  AAGTTTTCGCTACATCCCTGCCCTGTGTATGGGCATCTGCCGCGAAACCTGTCGGCCTGTTCATGGGAGT
  ACATGGGAACAGGCTCCCCTGAAAAAGCTGCTGGGCTTCCGAACCATTTGCATGGGGATTCTCCGTTTCG
  GATTCATCCACAAAACGGCGTTGGCATTGAACTTCTGGAATTGCAGGCCCCCGCAATTCCGCGAACCTGA
  CCTTCAGGCCCGGACATTCTGTTGAATGCCAATTCACCGCCTAGAGATCTCTGATTTGGCTCGGGCAAGT
  TGACACGGGTGTGTCCCACCGGCGAGACCTAGACAGGAGATGCCCGGTTGGCAAACGCGTCATCTTCTAC
  AGAGCATTCGAGAGAGACCGGGCTTCTAAACCAAACACAGTATAAAACGTTTCGATATCACTCGGGCGGC
  TCACAGAGAACGCTTTGATAGAGAAACTCTGCACACTGTGAAGTGTGAAGCCGCCCAATCCCCGTGTGAA
  CGCGCCTGAACTGGCTTACAGCTGCAAGATCAATTAACTCGAACTTTGAGCACGTGAAACTGCGAGTGAG
  GCTGACTGGT
  
  Kinase domain sequence

  >SC323692 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CSAAKGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGT
  CAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCGACGGCGGGACCCCATG
  GGCCCTGGCGCTGCTGCGCACCTTCGACGCGGGCGAGTTCACGGGCTGGGAGAAGGTGGGCTCGGGCGGC
  TTCGGGCAGGTGTACAAGGTGCGCCATGTCCACTGGAAGACCTGG
  
• Restriction Sites: Please inquire
• ACCN: NM_020639
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020639.2, NP_065690.2
• RefSeq Size: 3890 bp
• RefSeq ORF: 2355 bp
• Locus ID: 54101
• Cytogenetics: 21q22.3
• Domains: pkinase, TyrKc, ANK, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene is a serine/threonine protein kinase that interacts with protein kinase C-delta. The encoded protein can also activate NFkappaB and is required for keratinocyte differentiation. This kinase undergoes autophosphorylation. [provided by RefSeq, Jul 2008]