RSK4 (RPS6KA6) (NM_014496) Human Untagged Clone

CAT#: SC323689

RPS6KA6 (untagged)-Kinase deficient mutant (K455M) of Human ribosomal protein S6 kinase, 90kDa, polypeptide 6 (RPS6KA6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RSK4
• Synonyms: p90RSK6; PP90RSK4; RSK-4; RSK4; S6K-alpha-6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323689 sequence for NM_014496 edited (data generated by NextGen Sequencing)
  ATGCTACCATTCGCTCCTCAGGACGAGCCCTGGGACCGAGAAATGGAAGTGTTCAGCGGC
  GGCGGCGCGAGCAGCGGCGAGGTAAATGGTCTTAAAATGGTTGATGAGCCAATGGAAGAG
  GGAGAAGCAGATTCTTGTCATGATGAAGGAGTTGTTAAAGAAATCCCTATTACTCATCAT
  GTTAAGGAAGGCTATGAGAAAGCAGATCCTGCACAGTTTGAGTTGCTCAAGGTTCTTGGT
  CAGGGGTCATTTGGAAAGGTTTTTCTTGTTAGAAAGAAGACCGGTCCTGATGCTGGGCAG
  CTCTATGCAATGAAGGTGTTAAAAAAAGCCTCTTTAAAAGTTCGAGACAGAGTTCGGACA
  AAGATGGAGAGGGATATACTGGTGGAAGTAAATCATCCATTTATTGTCAAATTGCACTAT
  GCCTTTCAGACTGAAGGGAAACTGTACTTAATACTGGATTTTCTCAGGGGAGGAGATGTT
  TTCACAAGATTATCCAAAGAGGTTCTGTTTACAGAGGAAGATGTGAAATTCTACCTCGCA
  GAACTGGCCCTTGCTTTGGATCATCTGCACCAATTAGGAATTGTTTATAGAGACCTGAAG
  CCAGAAAACATTTTGCTTGATGAAATAGGACATATCAAATTAACAGATTTTGGACTCAGC
  AAGGAGTCAGTAGATCAAGAAAAGAAGGCTTACTCATTTTGTGGTACAGTAGAGTATATG
  GCTCCTGAAGTAGTAAATAGGAGAGGCCATTCCCAGAGTGCTGATTGGTGGTCATATGGT
  GTTCTTATGTTTGAAATGCTTACTGGTACTCTGCCATTTCAAGGTAAAGACAGAAATGAG
  ACCATGAATATGATATTAAAAGCAAAACTTGGAATGCCTCAATTTCTTAGTGCTGAAGCA
  CAAAGTCTTCTAAGGATGTTATTCAAAAGGAATCCAGCAAATAGATTGGGATCAGAAGGA
  GTTGAAGAAATCAAAAGACATCTGTTTTTTGCAAATATTGACTGGGATAAATTATATAAA
  AGAGAAGTTCAACCTCCTTTCAAACCTGCTTCTGGAAAACCAGATGATACTTTTTGTTTT
  GATCCTGAATTTACTGCAAAAACACCTAAAGATTCTCCCGGTTTGCCAGCCAGTGCAAAT
  GCTCATCAGCTCTTCAAAGGATTCAGCTTTGTTGCAACTTCTATTGCAGAAGAATATAAA
  ATCACTCCTATCACAAGTGCAAATGTATTACCAATTGTTCAGATAAATGGAAATGCTGCA
  CAATTTGGTGAAGTATATGAATTGAAGGAGGATATTGGTGTTGGCTCCTACTCTGTTTGC
  AAGCGATGCATACATGCAACTACCAACATGGAATTTGCAGTGATGATCATTGACAAAAGT
  AAGCGAGACCCTTCAGAAGAGATTGAAATATTGATGCGCTATGGACAACATCCCAACATT
  ATTACTTTGAAGGATGTCTTTGATGATGGTAGATATGTTTACCTTGTTACGGATTTAATG
  AAAGGAGGAGAGTTACTTGACCGTATTCTCAAACAAAAATGTTTCTCGGAACGGGAGGCT
  AGTGATATACTATATGTAATAAGTAAGACAGTTGACTATCTTCATTGTCAAGGAGTTGTT
  CATCGTGATCTTAAACCTAGTAATATTTTATACATGGATGAATCAGCCAGTGCAGATTCA
  ATCAGGATATGTGATTTTGGGTTTGCAAAACAACTTCGAGGAGAAAATGGACTTCTCTTA
  ACTCCATGCTACACTGCAAACTTTGTTGCACCTGAGGTTCTTATGCAACAGGGATATGAT
  GCTGCTTGTGATATCTGGAGTTTAGGAGTCCTTTTTTACACAATGTTGGCTGGCTACACT
  CCATTTGCTAATGGCCCCAATGATACTCCTGAAGAGATACTGCTGCGTATAGGCAATGGA
  AAATTCTCTTTGAGTGGTGGAAACTGGGACAATATTTCAGACGGAGCAAAGGATTTGCTT
  TCCCATATGCTTCATATGGACCCACATCAGCGGTATACTGCTGAACAAATATTAAAGCAC
  TCATGGATAACTCACAGAGACCAGTTGCCAAATGATCAGCCAAAGAGAAATGATGTGTCA
  CATGTTGTTAAGGGAGCAATGGTTGCAACATACTCTGCCCTGACTCACAAGACCTTTCAA
  CCAGTCCTAGAGCCTGTAGCTGCTTCAAGCTTAGCCCAGCGACGGAGCATGAAAAAGCGA
  ACATCAACTGGCCTGTAA
  
  Clone variation with respect to NM_014496.4
  1364 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_014496 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCTCCGTGGAGTGG
  GCAGGTTTACCGAGGCGCTAGCAGGGGCTTTTACTACTCTTCAGTCGCCTCCCCAGCCCAGCTCCCAGCC
  TCTGAGAGACCCGGCGGCGGCGGCGGCGGGCCAGTTCAATAGACAGGATGCGCGGCCGCGCCAGCGGTAG
  GCGGCAGCTCCTGTTTGAGTGCTCCTGAAGGGAGATGCTACCATTCGCTCCTCAGGACGAGCCCTGGGAC
  CCGAGAAATGGGAAGTGTTCAGGCGGCCGGCGGGCGCGAGCAGCGGCGAAGGTAAAATGTCCTTAAAATG
  TTTGATGAGCCAAGGAAAAGGGGAGAAGGCAGAATTCTTGGTCTGAATGAAGGAGTGGTTAAGGAATTCC
  TTTTAACCAATATTTGTAAGGGAAGGTAATGAAAAGCAGTTCCGGCCCGTTTAGTTGGCTCAGGGTTTTT
  GTCAGGGTCTATTTGAAAGGTTTCTCTGTTAGAAAAAAACGGCCGTATGCTGGGCGCTTATGCTGAAAGT
  GTAAAAAACCTTTTAAATGTCGAGACAGATTGCGAAGAGAAGAAGGGAATCGTGGAAGATACTCACTATA
  TGGCAATTCCATGCCCTGAACTAGGAACTTGCTATACTGAATTCTAGGGAGAAGTTTCCAGATTCCAAGT
  TGTTCAAGAGATGAAATCTCGAATGCTGTCGTACTGCCATAGGATGGAGTCAACACACGTGTGGAAAGAA
  CAATGAATTGGGCCAAGCGTGTCAACAA
  
  Kinase domain sequence

  >SC323689 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  AGAGAGWTTGGTGTTGGCTCCTACTCTGTTTGCAAGCGATGCATACATGCAACTACCAACATGGAATTTG
  CAGTGATGATCATTGACAAAAGTAAGCGAGACCCTTCAGAAGAGATTGAAATATTGATGCGCTATGGACA
  ACATCCCAACATTATTACTTTGAAGGATGTCTTTGATGATGGTAGATATGTTTACCTTGTTACGGATTTA
  ATGAAAGGAGGAGAGTTACTTGACCGTATTCTCAAACAAAAATGT
  
• Restriction Sites: Please inquire
• ACCN: NM_014496
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014496.1, NP_055311.1
• RefSeq Size: 2640 bp
• RefSeq ORF: 2238 bp
• Locus ID: 27330
• UniProt ID: Q9UK32
• Cytogenetics: Xq21.1
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Long-term potentiation, MAPK signaling pathway, mTOR signaling pathway, Neurotrophin signaling pathway, Oocyte meiosis, Progesterone-mediated oocyte maturation
• Gene Summary: This gene encodes a member of ribosomal S6 kinase family, serine-threonine protein kinases which are regulated by growth factors. The encoded protein may be distinct from other members of this family, however, as studies suggest it is not growth factor dependent and may not participate in the same signaling pathways. [provided by RefSeq, Jan 2010]
  Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, compared to variant 1. The encoded isoform (2) is the same length as isoform 1, but has a distinct N-terminus. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.