CSNK1G1 (NM_022048) Human Untagged Clone

CAT#: SC323608

CSNK1G1 (untagged)-Kinase deficient mutant (K73M) of Human casein kinase 1, gamma 1 (CSNK1G1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CSNK1G1
• Synonyms: CK1gamma1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323608 sequence for NM_022048 edited (data generated by NextGen Sequencing)
  ATGGACCATCCTAGTAGGGAAAAGGATGAAAGACAACGGACAACTAAACCCATGGCACAA
  AGGAGTGCACACTGCTCTCGACCATCTGGCTCCTCATCGTCCTCTGGGGTTCTTATGGTG
  GGACCCAACTTCAGGGTTGGCAAGAAGATAGGATGTGGGAACTTCGGAGAGCTCAGATTA
  GGTAAAAATCTCTACACCAATGAATATGTAGCAATCAAACTGGAACCAATAAAATCACGT
  GCTCCACAGCTTCATTTAGAGTACAGATTTTATAAACAGCTTGGCAGTGCAGGTGAAGGT
  CTCCCACAGGTGTATTACTTTGGACCATGTGGGAAATATAATGCCATGGTGCTGGAGCTC
  CTTGGCCCTAGCTTGGAGGACTTGTTTGACCTCTGTGACCGAACATTTACTTTGAAGACG
  GTGTTAATGATAGCCATCCAGCTGCTTTCTCGAATGGAATACGTGCACTCAAAGAACCTC
  ATTTACCGAGATGTCAAGCCAGAGAACTTCCTGATTGGTCGACAAGGCAATAAGAAAGAG
  CATGTTATACACATTATAGACTTTGGACTGGCCAAGGAATACATTGACCCCGAAACCAAA
  AAACACATACCTTATAGGGAACACAAAAGTTTAACTGGAACTGCAAGATATATGTCTATC
  AACACGCATCTTGGCAAAGAGCAAAGCCGGAGAGATGATTTGGAAGCCCTAGGCCATATG
  TTCATGTATTTCCTTCGAGGCAGCCTCCCCTGGCAAGGACTCAAGGCTGACACATTAAAA
  GAGAGATATCAAAAAATTGGTGACACCAAAAGGAATACTCCCATTGAAGCTCTCTGTGAG
  AACTTTCCAGAGGAGATGGCAACCTACCTTCGATATGTCAGGCGACTGGACTTCTTTGAA
  AAACCTGATTATGAGTATTTACGGACCCTCTTCACAGACCTCTTTGAAAAGAAAGGCTAC
  ACCTTTGACTATGCCTATGATTGGGTTGGGAGACCTATTCCTACTCCAGTAGGGTCAGTT
  CACGTAGATTCTGGTGCATCTGCAATAACTCGAGAAAGCCACACACATAGGGATCGGCCA
  TCACAACAGCAGCCTCTTCGAAATCAGGTGGTTAGCTCAACCAATGGAGAGCTGAATGTT
  GATGATCCCACGGGAGCCCACTCCAATGCACCAATCACAGCTCATGCCGAGGTGGAGGTA
  GTGGAGGAAGCTAAGTGCTGCTGTTTCTTTAAGAGGAAAAGGAAGAAGACTGCTCAGCGC
  CACAAGTGA
  
  Clone variation with respect to NM_022048.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_022048 unedited
  CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGTTCACCTAAGATTG
  AGACCTAGTGACTACATTTCCTACGGGAACAAATAAATGGTTTTTCATCTCCCGGAGATACATTACAAAC
  AAATATGGTGCTAAAAGAACTCCTTACCTTTCTCTGACTACAATTTATTTGGACATACTTTTGTATTGAA
  GAGAGGTATACATACTGAAGCTACTTGCTGTACTATAGGAGACTCTGTCCTGTAGGATCATGGACCATCC
  TAGTAGGGAAAAGGATGAAAGACAACGGACAACTAAAACCCATGGCAACAAAGGAGTGCACACTGCTCTC
  GACCATCTGGCTCCTCATCGTCCCTCTGGGGTTCCTTATGGGTGGGACCCCACTTTCAGGGTTGGCAAAG
  AAGATAGGATGGTGGGACCTCCGGAAGAGCTAGATAGGTAAAAATCTCTCCCCAATGAATTTGTAGCATT
  CATGCCTGAAACAATAAATCCCGTTCTCCCCGGCTTCATTAAAATCCGATTTATAAAACGCTGGGCAGTG
  CAGTGAAGGTTTCCAAAGTTGATTTCCTTTGACCCTGGGGGAAATAAATGCCTGGGTCGTGGACTCTTTG
  GCCCTCACTGAGAGCTTTGTTGACCTGTGACCGCACTTCTTTGAACCGGTATAGTACGCACCTCTCTCCC
  AGGGAACTGACTCAAACTAATACGAGTAGAGCGACTCGAGTGCCAGCATGAGACGTTAACTTAGCTGAAC
  GCGAACCTGGCGGACAGACATTAGAACAGATCAGATCGAGTCTCAC
  
  Kinase domain sequence

  >SC323608 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CTCGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGTTCACCTAAGATTGAGACCT
  AGTGACTACATTTCCTACGGGAACAAATAAATGGTTTTTCATCTCCCGGAGATACATTACAAACAAATAT
  GGTGCTAAAAGAACTCCTTACCTTTCTCTGACTACAATTTATTTG
  
• Restriction Sites: Please inquire
• ACCN: NM_022048
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022048.3, NP_071331.2
• RefSeq Size: 8163 bp
• RefSeq ORF: 1269 bp
• Locus ID: 53944
• UniProt ID: Q9HCP0
• Cytogenetics: 15q22.31
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Hedgehog signaling pathway
• Gene Summary: This gene encodes a member of the casein kinase I gene family. This family is comprised of serine/threonine kinases that phosphorylate acidic proteins such as caseins. The encoded kinase plays a role in cell cycle checkpoint arrest in response to stalled replication forks by phosphorylating Claspin. A mutation in this gene may be associated with non-syndromic early-onset epilepsy (NSEOE). [provided by RefSeq, Jul 2016]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the shortest isoform (1). Both variants 1 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.