CLK1 (NM_004071) Human Untagged Clone

CAT#: SC323558

CLK1 (untagged)-Kinase deficient mutant (K191M) of Human CDC-like kinase 1 (CLK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLK1
• Synonyms: CLK; CLK/STY; STY
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004071, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGACACTCAAAGAGAACTTACTGTCCTGATTGGGATGACAAGGATTGGGATTATGGAAAATGGAGGA
  GCAGCAGCAGTCATAAAAGAAGGAAGAGATCACATAGCAGTGCCCAGGAGAACAAGCGCTGCAAATACAA
  TCACTCTAAAATGTGTGATAGCCATTATTTGGAAAGCAGGTCTATAAATGAGAAAGATTATCATAGTCGA
  CGCTACATTGATGAGTACAGAAATGACTACACTCAAGGATGTGAACCTGGACATCGCCAAAGAGACCATG
  AAAGCCGGTATCAGAACCATAGTAGCAAGTCTTCTGGTAGAAGTGGAAGAAGTAGTTATAAAAGCAAACA
  CAGGATTCACCACAGTACTTCACATCGTCGTTCACATGGGAAGAGTCACCGAAGGAAAAGAACCAGGAGT
  GTAGAGGATGATGAGGAGGGTCACCTGATCTGTCAGAGTGGAGACGTACTAAGTGCAAGATATGAAATTG
  TTGATACTTTAGGTGAAGGAGCTTTTGGAAAAGTTGTGGAGTGCATCGATCATAAAGCGGGAGGTAGACA
  TGTAGCAGTAAAAATAGTTAAAAATGTGGATAGATACTGTGAAGCTGCTCGCTCAGAAATACAAGTTCTG
  GAACATCTGAATACAACAGACCCCAACAGTACTTTCCGCTGTGTCCAGATGTTGGAATGGTTTGAGCATC
  ATGGTCACATTTGCATTGTTTTTGAACTATTGGGACTTAGTACTTACGACTTCATTAAAGAAAATGGTTT
  TCTACCATTTCGACTGGATCATATCAGAAAGATGGCATATCAGATATGCAAGTCTGTGAATTTTTTGCAC
  AGTAATAAGTTGACTCACACAGACTTAAAGCCTGAAAACATCTTATTTGTGCAGTCTGACTACACAGAGG
  CGTATAATCCCAAAATAAAACGTGATGAACGCACCTTAATAAATCCAGATATTAAAGTTGTAGACTTTGG
  TAGTGCAACATATGATGACGAACATCACAGTACATTGGTATCTACAAGACATTATAGAGCACCTGAAGTT
  ATTTTAGCCCTAGGGTGGTCCCAACCATGTGATGTCTGGAGCATAGGATGCATTCTTATTGAATACTATC
  TTGGGTTTACCGTATTTCCAACACACGATAGTAAGGAGCATTTAGCAATGATGGAAAGGATTCTTGGACC
  TCTACCAAAACATATGATACAGAAAACCAGGAAACGTAAATATTTTCACCACGATCGATTAGACTGGGAT
  GAACACAGTTCTGCCGGCAGATATGTTTCAAGACGCTGTAAACCTCTGAAGGAATTTATGCTTTCTCAAG
  ATGTTGAACATGAGCGTCTCTTTGACCTCATTCAGAAAATGTTGGAGTATGATCCAGCCAAAAGAATTAC
  TCTCAGAGAAGCCTTAAAGCATCCTTTCTTTGACCTTCTGAAGAAAAGTATATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004071 unedited
  CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCGACGCAGTCAG
  CTGCGTGATTCCCGTGATTGCGTTACAAGCTTTGTCTCCTTCGACTTGGAGTCTTTGTCCAGGACGATGA
  GACACTCAAAGAGAACTTACTGTCCTGATTGGGATGACAAGGATTGGGATTATGGAAAATGGAGGAGCAG
  CAGCAGTCATAAAAGAAGGAAGAGATCACATAGCAGTGCCCATGAGAACAAGCGCTGCAAATACAATCAC
  TCTAAAATGTGTGATAGCCATTATTTGGAAAGCAGGTCTATAATGAAAAGGATTATCATAGTCGACCGCT
  ACATTGATGAGTCCGAAATGACTACACTCAGGATGTGACCCTGACATCCGCCAAAAAACCATGAAAGCGG
  TATAGACCATAGTAGCAAGTTCTTCTGGTTAAGTTGGAGAAAGGAGTTTAAAGCAACCCAGGATTACCAC
  GGTACTTACACTCTCGTCAAATGGGAAGATTACCAAGGAAGAACAGAGTGTAAGGAGAGAGAGGTCCCGT
  ATGGCAATGGAAGCTCATGGCAAAGAAATGTGTACTTGGAGAGCCTTGGAAATGTGATGTCACTCAACGA
  GAGGCGTGCCTATAGATTAATGGAAACCGGAGCTGCCAATACATTGAGAACTGAACAAAACAAGCTCCTG
  GCAGTGAGTGACTGCACTCTGTTAAATGCACTACACTAAAGGTTCTCTGTTACAAGAGCATACTCGGT
  
  Kinase domain sequence

  >SC323558 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TGRMGTCTAAGTGCAGATATGAATTGTTGATACTTTAGGTGAAGGAGCTTTTGGAAAAGTTGTGGAGTGC
  ATCGATCATAAAGCGGGAGGTAGGCATGTAGCAGTAATGATAGTTAAAAATGTGGATAGATACTGTGAAG
  CTGCTCGCTCAGAAATACAAGTTCTGGAACATCTGAATACAACAGACCCCAACAGTACTTTCCGCTGTGT
  CCAGATGTTGGAATGGTTTGAGCATCATGGTCACATTTGCATTGT
  
• Restriction Sites: Please inquire
• ACCN: NM_004071
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004071.2, NP_004062.2
• RefSeq Size: 1887 bp
• RefSeq ORF: 1455 bp
• Locus ID: 1195
• UniProt ID: P49759
• Cytogenetics: 2q33.1
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. In the nucleus, the encoded protein phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing, releasing them into the nucleoplasm. The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors, including serine/arginine rich proteins. Therefore, the encoded protein may play an indirect role in governing splice site selection. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]
  Transcript Variant: This variant (1) encodes the shorter isoform (1).