HIPK3 (NM_005734) Human Untagged Clone

CAT#: SC323551

HIPK3 (untagged)-Kinase deficient mutant (K226M) of Human homeodomain interacting protein kinase 3 (HIPK3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HIPK3
• Synonyms: DYRK6; FIST3; PKY; YAK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005734, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCTCACAAGTCTTGGTCTACCCACCATATGTTTATCAAACTCAGTCAAGTGCCTTTTGTAGTGTGA
  AGAAACTCAAAGTAGAGCCAAGCAGTTGTGTATTCCAGGAAAGAAACTATCCACGGACCTATGTGAATGG
  TAGAAACTTTGGAAATTCTCATCCTCCCACTAAGGGTAGTGCTTTTCAGACAAAGATACCATTTAATAGA
  CCTCGAGGACACAACTTTTCATTGCAGACAAGTGCTGTTGTTTTGAAAAACACTGCAGGTGCTACAAAGG
  TCATAGCAGCTCAGGCACAGCAAGCTCACGTGCAGGCACCTCAGATTGGGGCGTGGCGAAACAGATTGCA
  TTTCCTAGAAGGCCCCCAGCGATGTGGATTGAAGCGCAAGAGTGAGGAGTTGGATAATCATAGCAGCGCA
  ATGCAGATTGTCGATGAATTGTCCATACTTCCTGCAATGTTGCAAACCAACATGGGAAATCCAGTGACAG
  TTGTGACAGCTACCACAGGATCAAAACAGAATTGTACCACTGGAGAAGGTGACTATCAGTTAGTACAGCA
  TGAAGTCTTATGCTCCATGAAAAATACTTACGAAGTCCTTGATTTTCTTGGTCGAGGCACGTTTGGCCAG
  GTAGTTAAATGCTGGAAAAGAGGGACAAATGAAATTGTAGCAATCAAAATTTTGAAGAATCATCCTTCTT
  ATGCCCGTCAAGGTCAAATAGAAGTGAGCATATTAGCAAGGCTCAGTACTGAAAATGCTGATGAATATAA
  CTTTGTACGAGCTTATGAATGCTTTCAGCACCGTAACCATACTTGTTTAGTCTTTGAGATGCTGGAACAA
  AACTTGTATGACTTTCTGAAACAAAATAAATTTAGTCCCCTGCCACTAAAAGTGATTCGGCCCATTCTTC
  AACAAGTGGCCACTGCACTGAAAAAATTGAAAAGTCTTGGTTTAATTCATGCTGATCTCAAGCCAGAGAA
  TATTATGTTGGTGGATCCTGTTCGGCAGCCTTACAGGGTTAAAGTAATAGACTTTGGGTCGGCCAGTCAT
  GTATCAAAGACTGTTTGTTCAACATATCTACAATCTCGGTACTACAGAGCTCCAGAGATTATATTGGGGT
  TGCCATTTTGTGAAGCCATAGACATGTGGTCATTGGGATGTGTGATTGCAGAATTATTTCTTGGATGGCC
  GCTCTACCCAGGAGCCTTGGAGTATGATCAGATTCGATACATTTCTCAGACTCAAGGTTTGCCAGGAGAA
  CAGTTGTTAAATGTGGGTACTAAATCCACAAGATTTTTTTGCAAAGAAACAGATATGTCTCATTCTGGTT
  GGAGATTAAAGACATTGGAAGAGCATGAGGCAGAGACAGGAATGAAGTCTAAAGAAGCCAGAAAATACAT
  TTTCAACAGTCTGGATGATGTAGCGCATGTGAACACAGTGATGGATTTGGAAGGAAGTGATCTTTTGGCT
  GAGAAAGCTGATAGAAGAGAATTTGTTAGTCTGTTGAAGAAAATGTTGCTGATTGATGCAGATTTAAGAA
  TTACTCCAGCTGAGACCCTGAACCATCCTTTTGTTAATATGAAACATCTTCTAGATTTCCCTCATAGCAA
  CCATGTAAAGTCCTGTTTTCATATTATGGATATTTGTAAGTCCCACCTAAATTCATGTGACACAAATAAT
  CACAACAAAACTTCACTTTTAAGACCAGTTGCTTCAAGCAGTACTGCTACACTGACTGCAAATTTTACTA
  AAATCGGAACATTAAGAAGTCAGGCATTGACCACATCTGCTCATTCAGTTGTGCACCATGGAATACCTCT
  GCAGGCAGGAACTGCTCAGTTTGGTTGTGGTGATGCTTTTCAGCAGACATTGATTATCTGTCCCCCAGCT
  ATTCAAGGTATTCCTGCAACACATGGTAAACCCACCAGTTATTCAATAAGGGTAGATAATACAGTTCCAC
  TTGTAACTCAGGCCCCAGCTGTGCAGCCACTACAGATCCGACCAGGAGTTCTTTCTCAGACGTGGTCTGG
  TAGAACACAGCAGATGCTGGTGCCTGCCTGGCAACAGGTGACACCCCTGGCTCCTGCTACTACTACACTA
  ACTTCTGAGAGTGTGGCTGGTTCACACAGGCTTGGAGACTGGGGGAAGATGATTTCATGCAGCAATCATT
  ATAACTCAGTGATGCCGCAGCCTCTTCTGACCAATCAGATAACTTTATCTGCCCCTCAGCCAGTTAGTGT
  GGGGATTGCACATGTTGTCTGGCCTCAGCCTGCCACTACCAAGAAAAATAAACAGTGCCAGAACAGAGGT
  ATTTTGGTAAAACTAATGGAATGGGAGCCAGGAAGAGAGGAAATAAATGCTTTCAGTTGGAGTAATTCAT
  TACAGAATACCAATATCCCACATTCAGCATTTATTTCTCCAAAGATAATTAATGGGAAAGATGTCGAGGA
  AGTAAGTTGTATAGAAACACAGGACAATCAGAACTCAGAAGGAGAGGCAAGAAATTGCTGTGAAACATCT
  ATCAGACAGGACTCTGATTCATCAGTTTCAGACAAACAGCGGCAAACCATCATTATTGCCGACTCCCCGA
  GTCCTGCAGTGAGTGTCATCACTATCAGCAGTGACACTGATGAGGAAGAGACTTCCCAGAGACATTCACT
  CAGAGAATGTAAAGGTAGTCTAGATTGTGAAGCTTGCCAGAGCACTTTGAATATTGATCGGATGTGTTCA
  TTAAGTAGTCCTGATAGTACTCTGAGTACCAGCTCCTCAGGGCAGTCCAGCCCATCCCCCTGCAAGAGAC
  CGAATAGTATGTCAGATGAAGAGCAAGAAAGTAGTTGTGATACGGTGGATGGCTCTCCGACATCTGACTC
  TTCCGGGCATGACAGTCCATTTGCAGAGAGCACTTTTGTGGAGGACACTCATGAAAACACAGAATTGGTA
  TCCTCTGCTGACACAGAAACCAAGCCAGCTGTCTGTTCTGTTGTGGTGCCACCAGTGGAACTAGAAAATG
  GCTTAAATGCCGATGAGCATATGGCAAACACAGATTCTATATGCCAGCCATTAATAAAAGGACGATCTGC
  CCCTGGAAGATTAAACCAGCCTTCTGCAGTGGGTACTCGTCAGCAAAAATTGACATCAGCATTCCAGCAG
  CAGCATTTGAACTTCAGTCAGGTTCAGCACTTTGGATCTGGGCATCAAGAGTGGAATGGAAACTTTGGGC
  ACAGAAGACAGCAAGCTTATATTCCTACTAGTGTTACCAGTAATCCATTCACTCTTTCTCATGGAAGTCC
  CAATCACACAGCAGTGCATGCCCACCTGGCTGGAAATACACACCTCGGAGGACAGCCTACTCTACTTCCA
  TACCCATCATCAGCCACCCTCAGTAGTGCTGCACCAGTGGCCCACCTGTTAGCCTCTCCGTGTACCTCAA
  GACCTATGTTACAGCATCCAACTTATAATATCTCCCATCCCAGTGGCATAGTTCACCAAGTCCCAGTGGG
  CTTAAATCCCCGTCTGTTACCATCCCCAACCATTCATCAGACTCAGTACAAACCAATCTTCCCACCACAT
  TCTTACATTGCAGCATCACCTGCATATACTGGATTTCCACTGAGTCCAACAAAACTCAGCCAGTATCCAT
  ATATGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_005734 unedited
  ACCCGCCCGTTGCAGCAATGGGCGGTAGGCGTGTACAGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAG
  TGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGAAGATGAGG
  GAGACGGGCCCGGCGCTTAGCAGCCAGAGCAGCAGCAGCAGCAGCAGCGGTCGGGGGAGGGTGTTTCGCC
  GTTTCCTCTCAGCCGCCAGGACAAGATGGCAGCGGCCGCGGAGAGGGGCTGAGCCCGGGCTGGGTGGTGC
  CGCCTGCTGAAGCGCCTGGCTCCCGGTCCCCGGCACGGCCCTGCGCCCCCCCCCCGGACATGCTCAGGGC
  TGCGGGCCGCCCGAAAAAGGAGAGAGCGCGGGCCTCTAGGAAGGTATGGCCTCCCAAGTCTGGGTCTAAC
  CCCCCATATGTTTATTCAAATCAGTCCAATGGCCTTTGGTATGGGGAGAAAACTCAAAGTAAAAGCCAAC
  CAGTTGGGTTTTCCAGGAAAAAAACTATCCCCCGGACCTTTGGGATGGGGAAAAACTTTGGAAAATTCCC
  ATCTTCCCCCAAGGGGTAGGCCTTTTCAAAACAAAAATCCCTTTTAATAGACCCTCGGGGACCAACTTTT
  TCATTTCCAAAAAAAGGCTTTTGTTTTGAAAAAACCCTGGAGGGGCCCCCAAAGGGTAAAAGCCTCTAGG
  GCACACAGTTCCCTGTCGCGGGCCCCTAAAAGGGGGGGTGTGGAAAAAAAAATGTGTTTTTCATAAGAGC
  CCCCCCCACATGTGTGTTTTGCGCCCCAGAGGAGTGAGGTGTGTGATAATCTATACGCCCAGTCGCGATG
  TGTGTCAAGATATTCGCACTCTCCTCCGCGTGTGTGACACACAGTGAGAATCCGCTGCGTGTTGACACAC
  CACAGCTCACAATTATTCCCCGGAGGAGAGGTATCTATATTATTCACGCGGAGTATTTCTCGCCGCAAAA
  TATACATGACTAG
  
  Kinase domain sequence

  >SC323551 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  ARTCTTGWTTTCTTGGTCGAGGCACGTTTGGCCAGGTAGTTAAATGCTGGAAAAGAGGGACAAATGAAAT
  TGTAGCAATCATGATTTTGAAGAATCATCCTTCTTATGCCCGTCAAGGTCAAATAGAAGTGAGCATATTA
  GCAAGGCTCAGTACTGAAAATGCTGATGAATATAACTTTGTACGAGCTTATGAATGCTTTCAGCACCGTA
  ACCATACTTGTTTAGTCTTTGAGATGCTGGAACAAAACTTGTATG
  
• Restriction Sites: Please inquire
• ACCN: NM_005734
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005734.2, NP_005725.2
• RefSeq Size: 3657 bp
• RefSeq ORF: 3648 bp
• Locus ID: 10114
• UniProt ID: Q9H422
• Cytogenetics: 11p13
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Gene Summary: Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.