VRK1 (NM_003384) Human Untagged Clone

CAT#: SC323490

VRK1 (untagged)-Kinase deficient mutant (K71M) of Human vaccinia related kinase 1 (VRK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VRK1
• Synonyms: PCH1; PCH1A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323490 sequence for NM_003384 edited (data generated by NextGen Sequencing)
  ATGCCTCGTGTAAAAGCAGCTCAAGCTGGAAGACAGAGCTCTGCAAAGAGACATCTTGCA
  GAACAATTTGCAGTTGGAGAGATAATAACTGACATGGCAAAAAAGGAATGGAAAGTAGGA
  TTACCCATTGGCCAAGGAGGCTTTGGCTGTATATATCTTGCTGATATGAATTCTTCAGAG
  TCAGTTGGCAGTGATGCACCTTGTGTTGTAATGGTGGAACCCAGTGACAATGGACCTCTT
  TTTACTGAATTAAAGTTCTACCAACGAGCTGCAAAACCAGAGCAAATTCAGAAATGGATT
  CGTACCCGTAAGCTGAAGTACCTGGGTGTTCCTAAGTATTGGGGGTCTGGTCTACATGAC
  AAAAATGGAAAAAGTTACAGGTTTATGATAATGGATCGCTTTGGGAGTGACCTTCAGAAA
  ATATATGAAGCAAATGCCAAAAGGTTTTCTCGGAAAACTGTCTTGCAGCTAAGCTTAAGA
  ATTCTGGATATTCTGGAATATATTCACGAGCATGAGTATGTGCATGGAGATATCAAGGCC
  TCAAATCTTCTTCTGAACTACAAGAATCCTGACCAGGTGTACTTGGTAGATTATGGCCTT
  GCTTATCGGTACTGCCCAGAAGGAGTTCATAAAGAATACAAAGAAGACCCCAAAAGATGT
  CACGATGGCACTATTGAATTCACGAGCATCGATGCACACAATGGCGTGGCCCCATCAAGA
  CGTGGTGATTTGGAAATACTTGGTTATTGCATGATCCAATGGCTTACTGGCCATCTTCCT
  TGGGAGGATAATTTGAAAGATCCTAAATATGTTAGAGATTCCAAAATTAGATACAGAGAA
  AATATTGCAAGTTTGATGGACAAATGTTTTCCTGAGAAAAACAAACCAGGTGAAATTGCC
  AAATACATGGAAACAGTGAAATTACTAGACTACACTGAAAAACCTCTTTATGAAAATTTA
  CGTGACATTCTTTTGCAAGGACTAAAAGCTATAGGAAGTAAGGATGATGGCAAATTGGAC
  CTCAGTGTTGTGGAGAATGGAGGTTTGAAAGCAAAAACAATAACAAAGAAGCGAAAGAAA
  GAAATTGAAGAAAGCAAGGAACCTGGTGTTGAAGATACGGAATGGTCAAACACACAGACA
  GAGGAGGCCATACAGACCCGTTCAAGAACCAGAAAGAGAGTCCAGAAGTAA
  
  Clone variation with respect to NM_003384.2
  212 a=>t;213 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_003384 unedited
  ACCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGGAAGTTCGTA
  CTGGGCAGAACGCGACGGGTCTGCGGCTTAGGTGAAAATGCCTCGTGTAAAAGCAGCTCAAGCTGGAAGA
  CAGAGCTCTGCAAAGAGACATCTTGCAGAACAATTTGCAGTTGGAGAGATAATAACTGACATGGCAAAAA
  AGGAATGGAAAGTAGGATTACCCATTGGCCAAGGAGGCTTTTGGCTGTATATATCTTTGCTGATATGAAT
  TCTTCAGAGTCAGTTGGCAGTGATGCACCTTGTGTTGTAATGGGTGGGAACCCAGTGACAATGGACCCTC
  TTTTTTACCTGATTTAAGTTTCTAACCAAACGAAGCTTGCAAAACCCAGAGAGCAAAATCAGAGAAATTG
  GAATCCGTATACCCGTTAAGCTGTGAGTGTACCTGGGGTGGTTCCTATAATTTATTGGGGGTCTCTGGGT
  CCTACTGTGAACAAAAAGGGGAAAAAGTTACAGGTTTTATGAATATTGGATCGCTTTGGGGAGTGACCTT
  CAAAAAATATATAAGGCAAATGCAAAAGTGTTTTTCTGGGAAAACTGTCTCGCGCTAACCCTAAAAATTT
  GGGAATCTGGGAATATTCCACACGAGATGTGCGCGTGGATATATAGGCTAATCTTCTGAAACTACAAACT
  CACACGTGTGCTGTGATATAGGCTCTTATAGACTGCGCCAGAGGTCTAAGAATATCAAGACCCCAGAGTA
  TGCAGCGCTCTTGATATTCACACGTTGCGCCATGCGGCGCTCTTACAGTGAAATGTAGAACTCGTGTAGT
  ACATCGTGCTGCCCCTCTTGAGAGAAGTGAGACACAC
  
  Kinase domain sequence

  >SC323490 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CATGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGGAAGTTCGTACTGGGCA
  GAACGCGACGGGTCTGCGGCTTAGGTGAAAATGCCTCGTGTAAAAGCAGCTCAAGCTGGAAGACAGAGCT
  CTGCAAAGAGACATCTTGCAGAACAATTTGCAGTTGGAGAGATAA
  
• Restriction Sites: Please inquire
• ACCN: NM_003384
• Insert Size: 1630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003384.2, NP_003375.1
• RefSeq Size: 1720 bp
• RefSeq ORF: 1191 bp
• Locus ID: 7443
• UniProt ID: Q99986
• Cytogenetics: 14q32.2
• Domains: pkinase, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq, Jul 2008]