Casein Kinase 1 alpha (CSNK1A1) (NM_001892) Human Untagged Clone

CAT#: SC323470

CSNK1A1 (untagged)-Kinase deficient mutant (K46M) of Human casein kinase 1, alpha 1 (CSNK1A1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Casein Kinase 1 alpha
• Synonyms: CK1; CK1a; CKIa; HEL-S-77p; HLCDGP1; PRO2975
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323470 sequence for NM_001892 edited (data generated by NextGen Sequencing)
  ATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTCGGAGGGAAATATAAACTGGTA
  CGGAAGATCGGGTCTGGCTCCTTCGGGGACATCTATTTGGCGATCAACATCACCAACGGC
  GAGGAAGTGGCAGTGAAGCTAGAATCTCAGAAGGCCAGGCATCCCCAGTTGCTGTACGAG
  AGCAAGCTCTATAAGATTCTTCAAGGTGGGGTTGGCATCCCCCACATACGGTGGTATGGT
  CAGGAAAAAGACTACAATGTACTAGTCATGGATCTTCTGGGACCTAGCCTCGAAGACCTC
  TTCAATTTCTGTTCAAGAAGGTTCACAATGAAAACTGTACTTATGTTAGCTGACCAGATG
  ATCAGTAGAATTGAATATGTGCATACAAAGAATTTTATACACAGAGACATTAAACCAGAT
  AACTTCCTAATGGGTATTGGGCGTCACTGTAATAAGTTATTCCTTATTGATTTTGGTTTG
  GCCAAAAAGTACAGAGACAACAGGACAAGGCAACACATACCATACAGAGAAGATAAAAAC
  CTCACTGGCACTGCCCGATATGCTAGCATCAATGCACATCTTGGTATTGAGCAGAGTCGC
  CGAGATGACATGGAATCATTAGGATATGTTTTGATGTATTTTAATAGAACCAGCCTGCCA
  TGGCAAGGGCTAAAGGCTGCAACAAAGAAACAAAAATATGAAAAGATTAGTGAAAAGAAG
  ATGTCCACGCCTGTTGAAGTTTTATGTAAGGGGTTTCCTGCAGAATTTGCGATGTACTTA
  AACTATTGTCGTGGGCTACGCTTTGAGGAAGCCCCAGATTACATGTATCTGAGGCAGCTA
  TTCCGCATTCTTTTCAGGACCCTGAACCATCAATATGACTACACATTTGATTGGACAATG
  TTAAAGCAGAAAGCAGCACAGCAGGCAGCCTCTTCCAGTGGGCAGGGTCAGCAGGCCCAA
  ACCCCCACAGGCAAGCAAACTGACAAAACCAAGAGTAACATGAAAGGTTTCTAA
  
  Clone variation with respect to NM_001892.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_001892 unedited
  CCCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGACAGGGGTCCCT
  CTGGGCCGGAGCGCGGCGGCAGTGGTGGCAGCGGTATCGCCGCCCTAGCTCACCGCGCCCCTTTTCCAGC
  CCGCGACGTCGCCGCGCAAGCGAGGCAGCGGCGGCCGCCGAGAAACAAGTGGCCCAGCCTGGTAACCGCC
  GAGAAGCCCTTCACAAACTGCGGCCTGGCAAAAAGAAACCTGACTGAGCGGCGGTGATCAGGTGTCCCGC
  TCTGCTGATTCTGGGCCCCGAACCCCCGGTAAAGGCCTCCGTGTTCCGTTTTCCTGCCGCCCCTCCTCCG
  TAGCCCTTGCCTATGTAGGACCCCCGAGGCCTCCGTCCTCTTCCCAGAGGTTGTCGGGGCTTGCCCCAGC
  CCTCCATCTTCGTTCTCTAAGGATGGCAAGTAGCAGCGGGTCCAAGGCTGATTTCATTGCCGGGAGGGAA
  TTTACCTGGTACGGAGATCGGGTCTGGTCCCTCGGGACATATTTTGGCGTTAACTCCCACGGAGGGAATG
  GGCATGATGCTAATCTAAAGGGCGAGCTCAATGTGTGTCAGAGACAGCTATAGAGATTTCAAGGGGGTGG
  CCCACCATCGTGTGATGACGGAACACAACAGATATCTCGGCGTA
  
  Kinase domain sequence

  >SC323470 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CYATGMGCATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGACAGGGGTCCCTCTGGGCC
  GGAGCCGGCGGCAGTGGTGGCAGCGGTATCGCCGCCCTAGCTCACCGCGCCCCTTTTCCAGCCCGCGACG
  TCGCCGCGCAAGCGAGGCAGCGGCGGCCGCCGAGAAACAAGTGGC
  
• Restriction Sites: Please inquire
• ACCN: NM_001892
• Insert Size: 2630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001892.4, NP_001883.4
• RefSeq Size: 3065 bp
• RefSeq ORF: 1014 bp
• Locus ID: 1452
• UniProt ID: P48729
• Cytogenetics: 5q32
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Hedgehog signaling pathway, Wnt signaling pathway
• Gene Summary: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis (PubMed:11955436, PubMed:1409656, PubMed:18305108). May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration (PubMed:23902688).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame internal exon, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.