NUAK2 (NM_030952) Human Untagged Clone

CAT#: SC323459

NUAK2 (untagged)-Kinase deficient mutant (K81M) of Human NUAK family, SNF1-like kinase, 2 (NUAK2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NUAK2
• Synonyms: SNARK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_030952, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGTCGCTGGTTTTCGCGCGGCGCTCCGGCCCCACTCCCTCGGCCGCAGAGCTAGCCCGGCCGCTGG
  CGGAAGGGCTGATCAAGTCGCCCAAGCCCCTAATGAAGAAGCAGGCGGTGAAGCGGCACCACCACAAGCA
  CAACCTGCGGCACCGCTACGAGTTCCTGGAGACCCTGGGCAAAGGCACCTACGGGAAGGTGAAGAAGGCG
  CGGGAGAGCTCGGGGCGCCTGGTGGCCATCAAGTCAATCCGGAAGGACAAAATCAAAGATGAGCAAGATC
  TGATGCACATACGGAGGGAGATTGAGATCATGTCATCACTCAACCACCCTCACATCATTGCCATCCATGA
  AGTGTTTGAGAACAGCAGCAAGATCGTGATCGTCATGGAGTATGCCAGCCGGGGCGACCTTTATGACTAC
  ATCAGCGAGCGGCAGCAGCTCAGTGAGCGCGAAGCTAGGCATTTCTTCCGGCAGATCGTCTCTGCCGTGC
  ACTATTGCCATCAGAACAGAGTTGTCCACCGAGATCTCAAGCTGGAGAACATCCTCTTGGATGCCAATGG
  GAATATCAAGATTGCTGACTTCGGCCTCTCCAACCTCTACCATCAAGGCAAGTTCCTGCAGACATTCTGT
  GGGAGCCCCCTCTATGCCTCGCCAGAGATTGTCAATGGGAAGCCCTACACAGGCCCAGAGGTGGACAGCT
  GGTCCCTGGGTGTTCTCCTCTACATCCTGGTGCATGGCACCATGCCCTTTGATGGGCATGACCATAAGAT
  CCTAGTGAAACAGATCAGCAACGGGGCCTACCGGGAGCCACCTAAACCCTCTGATGCCTGTGGCCTGATC
  CGGTGGCTGTTGATGGTGAACCCCACCCGCCGGGCCACCCTGGAGGATGTGGCCAGTCACTGGTGGGTCA
  ACTGGGGCTACGCCACCCGAGTGGGAGAGCAGGAGGCTCCGCATGAGGGTGGGCACCCTGGCAGTGACTC
  TGCCCGCGCCTCCATGGCTGACTGGCTCCGGCGTTCCTCCCGCCCCCTCCTGGAGAATGGGGCCAAGGTG
  TGCAGCTTCTTCAAGCAGCATGCACCTGGTGGGGGAAGCACCACCCCTGGCCTGGAGCGCCAGCATTCGC
  TCAAGAAGTCCCGCAAGGAGAATGACATGGCCCAGTCTCTCCACAGTGACACGGCTGATGACACTGCCCA
  TCGCCCTGGCAAGAGCAACCTCAAGCTGCCAAAGGGCATTCTCAAGAAGAAGGTGTCAGCCTCTGCAGAA
  GGGGTACAGGAGGACCCTCCGGAGCTCAGCCCAATCCCTGCGAGCCCAGGGCAGGCTGCCCCGCTGCTCC
  CCAAGAAGGGCATTCTCAAGAAGCCCCGACAGCGCGAGTCTGGCTACTACTCCTCTCCCGAGCCCAGTGA
  ATCTGGGGAGCTCTTGGACGCAGGCGACGTGTTTGTGAGTGGGGATCCCAAGGAGCAGAAGCCTCCGCAA
  GCTTCAGGGCTGCTCCTCCATCGCAAAGGCATCCTCAAACTCAATGGCAAGTTCTCCCAGACAGCCTTGG
  AGCTCGCGGCCCCCACCACCTTCGGCTCCCTGGATGAACTCGCCCCACCTCGCCCCCTGGCCCGGGCCAG
  CCGACCCTCAGGGGCTGTGAGCGAGGACAGCATCCTGTCCTCTGAGTCCTTTGACCAGCTGGACTTGCCT
  GAACGGCTCCCAGAGCCCCCACTGCGGGGCTGTGTGTCTGTGGACAACCTCACGGGGCTTGAGGAGCCCC
  CCTCAGAGGGCCCTGGAAGCTGCCTGAGGCGCTGGCGGCAGGATCCTTTGGGGGACAGCTGCTTTTCCCT
  GACAGACTGCCAGGAGGTGACAGCGACCTACCGACAGGCACTGAGGGTCTGCTCAAAGCTCACCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_030952 unedited
  CCGCCCGTTCTCAGCAAATGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAG
  TGAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGGCTCCCC
  GTCCTGGTGCGGGACCTGTGCCCCGCGCTTCAGCCCTCCCCGCACAGCCTACTGATTCCCCTGCCGCCCT
  TGCTCACCTCCTGCTCGCCATGGAGTCGCTGGTTTTCGCGCGGCGCTCCGGCCCCACTCCCTCGGCCGCA
  GAGCTAGCCCGGCCGCTGGCGGAAGGGCTGATCAAGTCGCCCAAGCCCCTAATGAAGAAGCAGGCGGTGA
  AGCGGCACCACCACAAGCACAACCTGCGGCACCGCTACGAGTTCCTGGAGACCCTGGCAAAGGCACCTAC
  GGGAAGGTGAAGAGGCGCGGGAGAGCTCGGGCGCCTGTGGCCATCATGTCAATCCGGAGGACAAATTCAA
  AGATGAGCAGATCTGATGCAACATACGGAGGGAGATTGAAGATCATTGTCATCACTCACCACCTTCCAAT
  CATGCATCATGACTGTGTGGAAACAGCAGCAAGATCGGGATCGTCATGAATTATGCAGCTGGGCGGACTT
  ATTAGACCTCACTACCGACGCGCACACCGTCTATGTAGACGCGGAGCTAGGCATTCTTCCGGCATATCGT
  CTTTGGCTGTGCATATTGGCCCTCGAACAGATGTGCCCCGGATTCCACCTGGACACTCTTGTAGATCCAT
  GGGAATACGAATTGAATCTGCCCTCCAACTTACCAAGGAATTCGGACATCTTTGGGAACCTTAGCGCTCG
  AATGTAATGGAACCATCAAGCCAAGTGACAGTGCCGTGTTATCCTACGGTTAGCCAGCTTGGATCAGATC
  TAGCATTACCGTCTCGAT
  
  Kinase domain sequence

  >SC323459 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CYACKMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGGCTCCCCGTCCTGGTG
  CGGGACCTGTGCCCCGCGCTTCAGCCCTCCCCGCACAGCCTACTGATTCCCCTGCCGCCCTTGCTCACCT
  CCTGCTCGCCATGGAGTCGCTGGTTTTCGCGCGGCGCTCCGGCCC
  
• Restriction Sites: Please inquire
• ACCN: NM_030952
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030952.1, NP_112214.1
• RefSeq Size: 3443 bp
• RefSeq ORF: 1887 bp
• Locus ID: 81788
• UniProt ID: Q9H093
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cells from CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells. Able to phosphorylate 'Ser-464' of LATS1.[UniProtKB/Swiss-Prot Function]