TCF4 (NM_001083962) Human Untagged Clone

CAT#: SC316252

TCF4 (untagged)-Human transcription factor 4 (TCF4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCF4
• Synonyms: bHLHb19; CDG2T; E2-2; FECD3; ITF-2; ITF2; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; TCF-4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001083962 edited
  ATGGAGAGAGCGATGGAGCAACTCAACCGCCTGACGCGCTCGCTGCGCCGCGCGCGCACC
  GTGGAGTTGCCCGAGGATAATGAAACTGCTGTTTATACATTAATGCCAATGGTTATGGCT
  GATCAACACAGGTCTGTTTCTGAACTACTATCAAATTCAAAATTTGATGTCAATTATGCA
  TTCGGACGTGTGAAAAGAAGCTTGCTTCACATTGCAGCAAATTGTGGATCGGTGGAATGC
  TTGGTTTTGCTGTTAAAGAAAGGAGCAAATCCTAACTATCAAGATATTTCAGGCTGTACA
  CCCCTTCATTTGGCAGCAAGAAATGGGCAGAAGAAATGTATGAGTAAATTATTAGAATAT
  AGCGCTGATGTCAACATTTGTAATAATGAAGGCCTTACAGCAATACATTGGCTGGCTGTG
  AATGGGCGGACAGAACTACTCCATGACCTTGTGCAGCATGTCAGTGATGTTGATGTTGAG
  GATGCCATGGGGCAGACAGCACTGCATGTTGCCTGCCAGAACGGTCACAAGACGACAGTG
  CAGTGCTTGCTAGACAGTGGTGCTGATATTAACAGGCCAAATGTATCAGGAGCAACTCCA
  TTGTACTTTGCTTGCAGTCATGGTCAGAGAGATACAGCACAGATCCTACTATTACGAGGA
  GCCAAATATCTGCCAGATAAAAATGGAGTAACTCCTCTGGATTTATGTGTACAGGGTGGA
  TATGGAGAGACTTGTGAAGTATTAATTCAATATCACCCGAGGCTTTTTCAGACTATTATT
  CAAATGACACAGAATGAAGACCTCCGAGAAAACATGTTACGGCAAGTTCTGGAGCATTTG
  TCTCAGCAAAGTGAAAGCCAGTACCTAAAGATTCTAACAAGCCTTGCTGAAGTTGCTACA
  ACAAATGGTCATAAACTGCTTAGCCTCTCTAGCAATTATGATGCTCAAATGAAGAGCCTT
  TTAAGGATTGTGAGAATGTTTTGTCACGTCTTTCGAATTGGTCCATCCTCCCCCAGTAAT
  GGAATTGATATGGGCTACAATGGGAATAAAACTCCAAGAAGCCAGGTGTTCAAGCCTCTG
  GAATTGCTTTGGCACTCGTTAGATGAATGGCTAGTTTTAATAGCCACAGAATTGATGAAA
  AACAAAAGAGACTCAACAGAGATCACTTCTATTTTACTGAAACAAAAAGGCCAAGATCAA
  GATGCTGCTTCCATTCCTCCATTTGAACCTCCAGGACCTGGGAGCTATGAAAATCTGTCC
  ACTGGCACAAGGGAATCTAAACCAGATGCTCTTGCAGGGAGACAGGAAGCCAGTGCAGAT
  TGTCAGGATGTTATTTCTATGACAGCTAACCGGCTAAGTGCTGTCATTCAAGCTTTTTAC
  ATGTGCTGTTCTTGTCAGATGCCTCCGGGAATGACTTCACCTCGTTTCATTGAATTTGTC
  TGCAAACATGATGAAGTTTTAAAATGCTTTGTTAATAGAAATCCCAAAATTATATTTGAC
  CACTTTCACTTTCTCCTTGAATGTCCTGAGTTGATGTCAAGATTCATGCATATCATAAAA
  GCACAGCCTTTTAAAGATCGCTGTGAATGGTTCTATGAACATTTGCATTCAGGACAGCCA
  GATTCAGATATGGTGCACAGGCCAGTGAATGAAAATGATATCCTGCTGGTTCACAGAGAT
  TCTATTTTTAGGAGTAGCTGTGAAGTTGTGTCAAAAGCAAATTGTGCAAAGCTAAAGCAA
  GGGATTGCTGTACGGTTCCATGGAGAAGAAGGCATGGGTCAAGGTGTTGTGCGTGAGTGG
  TTTGATATTCTGTCCAATGAGATAGTCAATCCTGATTATGCATTGTTTACCCAGTCAGCT
  GATGGAACAACTTTTCAGCCTAATAGCAACTCTTATGTAAATCCTGATCACTTGAACTAT
  TTTCGGTTTGCTGGGCAGATCTTGGGATTAGCGTTGAACCACAGGCAGCTGGTCAATATT
  TACTTCACACGATCCTTCTACAAGCACATTCTTGGTATTCCTGTAAATTACCAAGATGTG
  GCATCCATTGATCCAGAATATGCGAAAAATTTGCAATGGATTTTAGATAATGATATAAGT
  GATCTGGGTCTAGAACTAACTTTTTCTGTTGAGACTGATGTGTTTGGAGCAATGGAAGAG
  GTGCCTTTGAAACCTGGGGGTGGGAGTATTCTTGTGACACAAAATAATAAAGCGGAGTAC
  GTCCAGCTTGTTACTGAACTTCGAATGACAAGAGCCATTCAGCCTCAGATCAATGCTTTT
  TTACAGGGCTTTCATATGTTCATTCCACCCTCCCTCATACAGCTTTTTGATGAATATGAA
  TTGGAGCTACTGCTTTCTGGCATGCCAGAAATTGATGTGAGTGATTGGATAAAAAATACA
  GAATACACAAGTGGCTATGAAAGAGAAGATCCAGTTATTCAGTGGTTCTGGGAAGTTGTA
  GAAGACATTACTCAAGAGGAGAGAGTTCTTCTCTTACAGTTTGTTACGGGCAGTTCCAGG
  GTCCCACATGGTGGGTTTGCTAATATCATGGGTGGAAGTGGATTGCAAAACTTTACAATC
  GCTGCTGTGCCATATACTCCAAATCTTTTACCAACTTCAAGCACATGCATCAACATGCTC
  AAGTTACCTGAATACCCAAGTAAAGAAATACTCAAGGACAGACTTCTTGTGGCACTACAT
  TGTGGCAGCTATGGTTACACAATGGCAACGCGTACGCGGCCGCTCGAGCAGAAACTCATC
  TCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTT
  TAAAC
  
• Restriction Sites: Please inquire
• ACCN: NM_001083962
• Insert Size: 2200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: ORF sequence matches with the reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001083962.1, NP_001077431.1
• RefSeq Size: 8332 bp
• RefSeq ORF: 2016 bp
• Locus ID: 6925
• UniProt ID: P15884
• Cytogenetics: 18q21.2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Gene Summary: This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. In addition, an intronic CTG repeat normally numbering 10-37 repeat units can expand to >50 repeat units and cause Fuchs endothelial corneal dystrophy. Multiple alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq, Jul 2016]
  Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (a, also known as TCF4-B+) is shorter at the N-terminus compared to isoform c. Sequence Note: This gene is distinct from TCF7L2 (alias TCF-4).