Cullin 4B (CUL4B) (NM_001079872) Human Untagged Clone

CAT#: SC315848

CUL4B (untagged)-Human cullin 4B (CUL4B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cullin 4B
• Synonyms: CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001079872, the custom clone sequence may differ by one or more nucleotides
  ATGTTTCCAACAGGTTTTTCTTCCCCCAGTCCCTCAGCTGCTGCTGCTGCTCAGGAGGTC
  AGATCTGCCACTGATGGTAATACCAGCACCACTCCGCCCACCTCTGCCAAGAAGAGAAAG
  TTAAACAGCAGCAGCAGTAGCAGCAGTAACAGTAGTAACGAGAGAGAAGACTTTGATTCC
  ACCTCTTCCTCCTCTTCCACTCCTCCTTTACAACCCAGGGATTCGGCATCCCCTTCAACC
  TCGTCCTTCTGCCTGGGGGTTTCAGTGGCTGCTTCCAGCCACGTACCGATACAGAAGAAG
  CTGCGTTTTGAAGACACCCTGGAGTTTGTAGGGTTTGATGCGAAGATGGCTGAGGAATCC
  TCCTCCTCCTCCTCCTCATCTTCACCAACTGCTGCAACATCTCAGCAGCAGCAACTTAAA
  AATAAGAGTATATTAATCTCTTCTGTGGCTTCGGTGCATCATGCAAACGGCCTAGCCAAA
  TCTTCTACCACCGTCTCTAGCTTTGCTAACAGCAAACCTGGCTCTGCTAAGAAGTTAGTG
  ATCAAGAACTTTAAAGATAAGCCTAAATTACCAGAAAACTACACAGATGAAACCTGGCAA
  AAACTGAAAGAAGCAGTGGAAGCTATTCAGAATAGTACTTCAATTAAGTACAATTTAGAA
  GAACTCTACCAGGCTGTAGAAAATCTCTGTTCTTACAAGATTTCTGCAAACTTGTACAAA
  CAGCTGAGACAGATCTGCGAAGATCACATCAAAGCACAGATTCATCAATTCAGAGAGGAT
  TCATTGGATAGCGTTCTTTTTTTAAAGAAGATTGATAGATGCTGGCAAAACCATTGCAGA
  CAAATGATCATGATCAGGAGCATTTTTTTGTTTCTGGATAGAACTTACGTTCTTCAGAAT
  TCAATGCTACCCTCCATTTGGGACATGGGACTGGAGTTATTTAGGGCTCATATTATAAGT
  GATCAGAAAGTGCAGAATAAGACAATTGATGGCATTCTTCTCTTGATTGAGAGGGAAAGG
  AATGGTGAAGCAATTGATAGAAGTTTACTTCGAAGCCTTTTAAGCATGCTGTCTGATTTG
  CAAATTTATCAAGATTCTTTTGAACAACGATTTTTGGAAGAAACTAACCGGCTCTATGCA
  GCTGAAGGCCAAAAATTAATGCAAGAAAGAGAGGTTCCTGAATATCTACATCATGTTAAC
  AAACGTCTAGAAGAAGAAGCAGACAGACTTATTACTTACTTAGATCAGACCACCCAGAAG
  TCATTAATTGCTACTGTAGAAAAACAACTTCTAGGTGAACACTTAACAGCAATTCTTCAG
  AAAGGTTTAAATAACCTCCTTGATGAAAACCGAATTCAAGATTTGTCTCTTCTGTATCAG
  CTCTTCAGTAGAGTTCGAGGTGGAGTTCAGGTTCTTTTGCAGCAGTGGATCGAATATATC
  AAGGCATTTGGCAGCACTATTGTAATTAATCCTGAAAAAGATAAAACCATGGTTCAAGAA
  TTGCTGGATTTTAAAGATAAGGTTGACCATATAATTGATATCTGCTTTCTGAAGAATGAG
  AAATTTATCAATGCCATGAAAGAAGCATTTGAAACGTTCATTAACAAAAGACCAAATAAA
  CCAGCTGAACTTATAGCTAAGTATGTAGATTCAAAACTTCGTGCAGGCAACAAAGAAGCT
  ACAGATGAAGAACTTGAGAAAATGTTGGATAAAATTATGATCATATTTAGATTTATCTAT
  GGCAAGGATGTTTTTGAGGCCTTCTATAAGAAAGATTTAGCCAAGCGCCTGTTAGTCGGA
  AAGAGTGCATCTGTAGATGCTGAAAAATCAATGCTGTCCAAACTTAAACATGAATGCGGA
  GCTGCTTTCACCAGCAAACTTGAAGGAATGTTTAAAGACATGGAACTTTCTAAAGACATC
  ATGATTCAGTTCAAACAGTATATGCAGAATCAGAATGTTCCGGGAAATATTGAGTTAACT
  GTGAATATCCTGACAATGGGCTATTGGCCGACATATGTGCCTATGGAAGTTCATTTACCA
  CCAGAGATGGTAAAACTTCAGGAGATTTTCAAGACATTTTACCTAGGCAAACATAGTGGC
  AGGAAACTTCAGTGGCAGTCAACCCTAGGACACTGTGTGTTAAAAGCAGAATTTAAAGAG
  GGTAAAAAGGAACTCCAGGTCTCTCTTTTTCAAACACTGGTGCTGCTAATGTTTAATGAG
  GGAGAGGAGTTCAGTTTAGAAGAGATCAAGCAGGCAACTGGAATAGAGGATGGAGAGTTA
  AGGAGAACACTGCAGTCATTAGCCTGTGGCAAAGCTAGAGTTCTGGCGAAAAATCCAAAG
  GGCAAAGACATTGAAGATGGTGACAAGTTCATTTGTAATGATGATTTCAAACATAAACTT
  TTCAGGATAAAGATCAATCAAATCCAGATGAAAGAAACGGTTGAAGAACAAGCAAGCACT
  ACAGAAAGAGTATTTCAAGACAGACAGTATCAAATTGATGCTGCAATTGTTCGAATTATG
  AAGATGAGAAAGACACTTAGCCACAATCTCCTTGTTTCAGAAGTGTACAACCAGTTGAAA
  TTTCCAGTAAAGCCTGCTGATCTTAAGAAGAGAATAGAATCTTTAATTGACCGGGACTAC
  ATGGAAAGAGATAAAGAAAATCCAAACCAGTACAACTATATTGCA
  
• Restriction Sites: Please inquire
• ACCN: NM_001079872
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079872.1, NP_001073341.1
• RefSeq Size: 5198 bp
• RefSeq ORF: 2688 bp
• Locus ID: 8450
• UniProt ID: Q13620
• Cytogenetics: Xq24
• Protein Pathways: Nucleotide excision repair, Ubiquitin mediated proteolysis
• Gene Summary: This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter and unique N-terminus, compared to isoform 1.