RFX3 (NM_002919) Human Untagged Clone

CAT#: SC314482

RFX3 (untagged)-Human regulatory factor X, 3 (influences HLA class II expression) (RFX3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RFX3
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002919, the custom clone sequence may differ by one or more nucleotides
  ATGCAGACATCAGAGACTGGGTCGGACACAGGCTCGACAGTGACCTTACAAACATCTGTG
  GCTAGTCAAGCAGCAGTGCCTACGCAGGTGGTACAGCAAGTACCAGTACAACAACAGGTA
  CAGCAGGTACAGACTGTGCAGCAGGTACAACATGTCTATCCCGCTCAGGTGCAGTATGTG
  GAAGGAAGCGATACTGTCTATACCAATGGAGCAATCCGAACAACAACGTATCCTTACACA
  GAGACACAGATGTACAGCCAAAATACTGGAGGGAATTACTTTGATACTCAAGGGAGTTCC
  GCCCAGGTGACTACCGTGGTCTCATCCCACAGTATGGTGGGCACTGGTGGGATTCAGATG
  GGCGTCACAGGAGGACAACTCATCAGCAGCTCTGGAGGAACCTATCTGATCGGCAACTCA
  ATGGAGAATTCTGGTCACTCAGTGACACACACAACTCGGGCCTCCCCAGCGACAATTGAA
  ATGGCGATTGAGACGCTGCAAAAGTCTGACGGTCTGTCCACTCACAGAAGCTCTCTTCTC
  AACAGCCATCTCCAGTGGCTGTTGGACAATTATGAGACAGCAGAAGGAGTGAGCCTTCCC
  AGAAGCACTCTGTACAACCACTACCTTCGACACTGTCAGGAACACAAACTGGACCCAGTC
  AATGCTGCCTCTTTTGGAAAATTAATAAGATCAATTTTTATGGGGCTACGAACCAGGAGA
  TTGGGCACTAGAGGAAACTCCAAATACCACTACTATGGGATTCGTGTCAAGCCAGATTCC
  CCTCTTAATCGTCTGCAAGAAGACATGCAGTATATGGCTATGAGACAACAACCCATGCAA
  CAGAAACAAAGGTACAAGCCTATGCAGAAAGTGGATGGGGTTGCAGATGGTTTCACAGGA
  AGTGGTCAACAGACAGGCACATCTGTTGAGCAAACTGTAATTGCCCAAAGCCAACATCAT
  CAACAGTTTTTAGATGCATCTCGAGCACTTCCAGAGTTTGGAGAAGTTGAAATCTCTTCT
  CTGCCAGATGGTACTACCTTTGAGGATATCAAGTCACTGCAGAGTCTTTATAGAGAGCAC
  TGTGAGGCAATATTGGACGTTGTTGTGAATCTTCAATTTAGCCTGATAGAAAAATTGTGG
  CAAACATTCTGGCGCTATTCTCCCTCTACTCCAACTGATGGCACTACCATTACCGAATCG
  AGCAATCTGAGTGAAATAGAAAGTCGACTTCCGAAAGCAAAGCTGATAACTCTGTGCAAA
  CATGAGTCTATCCTGAAATGGATGTGTAACTGTGACCATGGGATGTACCAGGCTTTGGTG
  GAGATTCTCATCCCCGACGTCCTTAGACCTATTCCTAGTGCCTTGACCCAAGCCATTCGA
  AATTTTGCAAAAAGCCTTGAAGGTTGGCTTTCCAATGCCATGAACAATATTCCACAGAGA
  ATGATACAAACCAAGGTTGCCGCTGTAAGTGCCTTTGCCCAGACTCTGCGAAGATACACG
  TCGCTTAATCACCTGGCCCAGGCAGCTCGTGCAGTGCTTCAGAACACTTCCCAAATCAAC
  CAGATGCTTAGTGACCTCAACCGTGTCGACTTTGCCAATGTCCAGGAGCAGGCTTCCTGG
  GTGTGCCAGTGTGATGACAACATGGTTCAGAGACTAGAAACAGACTTCAAGATGACTCTT
  CAGCAGCAGAGCACCCTGGAGCAGTGGGCTGCGTGGCTTGACAATGTGATGATGCAAGCA
  CTGAAACCCTATGAAGGAAGACCCAGTTTTCCTAAAGCCGCCAGGCAGTTTCTGCTAAAA
  TGGTCTTTCTACAGCTCAATGGTTATTCGGGACTTAACCTTACGCAGTGCTGCTAGCTTT
  GGCTCCTTCCACCTGATCCGTCTACTCTACGACGAATATATGTTTTACTTAGTAGAACAT
  CGTGTTGCTCAGGCAACAGGAGAGACTCCTATAGCAGTCATGGGCGAGGTAAGAGAGGCT
  GAAAGAGCTGTGACCCACTGGGTTATTAAAAATAAGCCAGAATTACACTTCAGTCTAAAT
  ACATTATTGATTAAAACCATGGTTCCTAACCAAGTCAGCCTCAGAGCCAGGAGGGACTGT
  GGAGTTATTGCAAGAGTTCCT
  
• Restriction Sites: Please inquire
• ACCN: NM_002919
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002919.2, NP_002910.1
• RefSeq Size: 3275 bp
• RefSeq ORF: 2124 bp
• Locus ID: 5991
• UniProt ID: P48380
• Cytogenetics: 9p24.2
• Domains: RFX_DNA_binding, RFX1_trans_act
• Protein Families: Transcription Factors
• Gene Summary: This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (1) differs in the 5' UTR and its 3' terminal exon extends past a splice site that is used in variant 2. This results in a novel 3' coding region and 3' UTR, compared to variant 2. It encodes isoform a which is shorter and has a distinct C-terminus, compared to isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.