GABRG3 (NM_033223) Human Untagged Clone

CAT#: SC313609

GABRG3 (untagged)-Human gamma-aminobutyric acid (GABA) A receptor, gamma 3 (GABRG3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GABRG3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_033223 edited
  ATGGCCCCGAAGCTGCTGCTCCTCCTCTGCCTGTTCTCGGGCTTGCACGCGCGGTCCAGA
  AAGGTGGAAGAGGATGAATATGAAGATTCATCATCAAACCAAAAGTGGGTCTTGGCTCCA
  AAATCCCAAGACACCGACGTGACTCTTATTCTCAACAAGTTGCTAAGAGAATATGATAAA
  AAGCTGAGGCCAGATATTGGAATAAAACCGACCGTAATTGACGTTGACATTTATGTTAAC
  AGCATTGGTCCTGTGTCATCAATAAACATGGAATACCAAATTGACATATTTTTTGCTCAG
  ACCTGGACAGATAGTCGCCTTCGATTCAACAGCACAATGAAAATTCTTACTCTGAACAGC
  AACATGGTGGGGTTAATCTGGATCCCAGACACCATCTTCCGCAATTCTAAAACCGCAGAG
  GCTCACTGGATCACCACACCCAATCAGCTCCTCCGGATTTGGAATGACGGGAAAATCCTT
  TACACTTTGAGGCTCACCATCAATGCTGAGTGCCAGCTGCAGCTGCACAACTTCCCCATG
  GACGAACACTCCTGCCCGCTGATTTTCTCCAGCTATGGCTATCCCAAAGAAGAAATGATT
  TATAGATGGAGAAAAAATTCAGTGGAGGCAGCTGACCAGAAATCATGGCGGCTTTATCAG
  TTTGACTTCATGGGCCTCAGAAACACCACAGAAATCGTGACAACGTCTGCAGGTGATTAT
  GTTGTCATGACTATATATTTTGAATTGAGTAGAAGAATGGGATACTTCACCATTCAGACA
  TACATTCCCTGTATACTGACTGTGGTTTTATCCTGGGTGTCATTTTGGATCAAAAAAGAT
  GCTACGCCAGCAAGAACAGCATTAGGCATCACCACGGTGCTGACCATGACCACCCTGAGC
  ACCATCGCCAGGAAGTCCTTGCCACGCGTGTCCTACGTGACCGCCATGGACCTTTTTGTG
  ACCGTGTGCTTCCTGTTTGTCTTCGCCGCGCTGATGGAGTATGCCACCCTCAACTACTAT
  TCCAGCTGTAGAAAACCAACCACCACGAAGAAGACAACATCGTTACTACATCCAGATTCC
  TCAAGATGGATTCCTGAGCGAATAAGCCTACAAGCCCCTTCCAACTATTCCCTCCTGGAC
  ATGAGGCCACCACCAACTGCGATGATCACTTTAAACAATTCCGTTTACTGGCAGGAATTT
  GAAGATACCTGTGTCTATGAGTGTCTGGATGGCAAAGACTGTCAGAGCTTCTTCTGCTGC
  TATGAAGAATGTAAATCAGGATCCTGGAGGAAAGGGCGTATTCACATAGACATCTTGGAG
  CTGGACTCGTACTCCCGGGTCTTTTTCCCCACGTCCTTCCTGCTCTTTAACCTGGTCTAC
  TGGGTTGGATACCTGTATCTCTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033223 unedited
  GTCAGATTAGGTATACGACTTATATAGGCGGCCGCGNAATTCGCACGAGGGCCGCCCGGT
  TGCGCGGACCGGCGCTAGTGCGCGGGTGGGGGCGGCGCGCCGCGGTGGCCCGGCGTCCCG
  TGTGCGTCCAGTGTGCGCCCCGCGGGGGCGCGGCCAGCGCCAGAGTAGATACCTGTCCCG
  CCCGCCGCGGCCAGCAGCCTCGGAGGAAGCCAGGGCAAAGAGGGCCGGCGGAGACCAGGT
  CCGCGCCGGAGGAAGCCGCGCCCGGCCGAGGCCCCGGACCCTGCGCCCCGAGCTCCACGG
  CACCATGGCCCCGAAGCTGCTGCTCCTCCTCTGCCTGTTCTCGGGCTTGCACGCGCGGTC
  CAGAAAGGTGGAAGAGGATGAATATGAAGATTCATCATCAAACCAAAAGTGGGTCTTGGC
  TCCAAAATCCCAAGACACCGACGTGACTCTTATTCTCAACAAGTTGCTAAGAGAATATGA
  TAAAAAGCTGAGGCCAGATATTGGAATAAAACCGACCGTAATTGACGTTGACATTTATGT
  TAACAGCATTGGTCCTGTGTCATCAATAAACATGGAATACCAAATTGACATATTTTTTGC
  TCAGACCTGGACAGATAGTCGCCTTCGATTCAACAGCACAATGAAAATTCTTACTCTGAA
  CAGCAACATGGTGGGGTTAATCTGGATCCCAGACACCATCTTCCGCAATTCTAAAACCGC
  AGAGGCTCACTGGATCACCACACCCAATCAGCTCCTNCGGATTTGGAATGACGGNAAAAA
  CCTTTACACTTTGAGGCTCACCATCAATGCTGAGTGCCAGCTGCAGCTGCACAACTTCCC
  ATGGACGAACACTTCTGCCCGCTGATTNTCTCCAGCTTGGCTATCCCNAAGGAGAAATGA
  TTATTAGTTGAGAAAAAATTCATGGAGCAGCTGACCANAATNATGGCGGGTTTATAGTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033223
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: It is not a varient.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033223.1, NP_150092.1
• RefSeq Size: 1536 bp
• RefSeq ORF: 1404 bp
• Locus ID: 2567
• UniProt ID: Q99928
• Cytogenetics: 15q12
• Domains: Neur_chan_memb, Neur_chan_LBD
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. The protein encoded by this gene is a gamma subunit, which contains the benzodiazepine binding site. Two transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Aug 2012]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).