NRG1 (NM_013959) Human Untagged Clone

CAT#: SC312845

NRG1 (untagged)-Human neuregulin 1 (NRG1), transcript variant SMDF

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NRG1
• Synonyms: ARIA; GGF; GGF2; HGL; HRG; HRG1; HRGA; MST131; MSTP131; NDF; NRG1-IT2; SMDF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_013959 edited
  ATGGAGATTTATTCCCCAGACATGTCTGAGGTCGCCGCCGAGAGGTCCTCCAGCCCCTCC
  ACTCAGCTGAGTGCAGACCCATCTCTTGATGGGCTTCCGGCAGCAGAAGACATGCCAGAG
  CCCCAGACTGAAGATGGGAGAACCCCTGGACTCGTGGGCCTGGCCGTGCCCTGCTGTGCG
  TGCCTAGAAGCTGAGCGCCTGAGAGGTTGCCTCAACTCAGAGAAAATCTGCATTGTCCCC
  ATCCTGGCTTGCCTGGTCAGCCTCTGCCTCTGCATCGCCGGCCTCAAGTGGGTATTTGTG
  GACAAGATCTTTGAATATGACTCTCCTACTCACCTTGACCCTGGGGGGTTAGGCCAGGAC
  CCTATTATTTCTCTGGACGCAACTGCTGCCTCAGCTGTGTGGGTGTCGTCTGAGGCATAC
  ACTTCACCTGTCTCTAGGGCTCAATCTGAAAGTGAGGTTCAAGTTACAGTGCAAGGTGAC
  AAGGCTGTTGTCTCCTTTGAACCATCAGCGGCACCGACACCGAAGAATCGTATTTTTGCC
  TTTTCTTTCTTGCCGTCCACTGCGCCATCCTTCCCTTCACCCACCCGGAACCCTGAGGTG
  AGAACGCCCAAGTCAGCAACTCAGCCACAAACAACAGAAACTAATCTCCAAACTGCTCCT
  AAACTTTCTACATCTACATCCACCACTGGGACAAGCCATCTTGTAAAATGTGCGGAGAAG
  GAGAAAACTTTCTGTGTGAATGGAGGGGAGTGCTTCATGGTGAAAGACCTTTCAAACCCC
  TCGAGATACTTGTGCAAGTGCCCAAATGAGTTTACTGGTGATCGCTGCCAAAACTACGTA
  ATGGCCAGCTTCTACAGTACGTCCACTCCCTTTCTGTCTCTGCCTGAATAG
  
• Restriction Sites: Please inquire
• ACCN: NM_013959
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013959.1, NP_039253.1
• RefSeq Size: 1860 bp
• RefSeq ORF: 891 bp
• Locus ID: 3084
• UniProt ID: Q02297
• Cytogenetics: 8p12
• Domains: EGF, EGF
• Protein Families: Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
• Protein Pathways: ErbB signaling pathway
• Gene Summary: The protein encoded by this gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. An extraordinary variety of different isoforms are produced from this gene through alternative promoter usage and splicing. These isoforms are expressed in a tissue-specific manner and differ significantly in their structure, and are classified as types I, II, III, IV, V and VI. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). [provided by RefSeq, Apr 2016]
  Transcript Variant: This variant (SMDF), which uses the type III promoter, is expressed mainly in the nervous system. It lacks multiple 5' and 3' exons but has alternate 5' and 3' terminal exons, and it thus differs in the 5' and 3' coding regions and in both UTRs, compared to variant HRG-beta1. The resulting isoform (SMDF, also known as NRG-III-beta3) has distinct N- and C-termini and is shorter than isoform HRG-beta1.