TGF alpha (TGFA) (NM_003236) Human Untagged Clone

CAT#: SC311226

TGFA (untagged)-Human transforming growth factor, alpha (TGFA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TGF alpha
• Synonyms: TFGA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003236 edited
  GCCATTACGGCCGGGGACTCGGGCACCGCAGGTAGGGCAGGAGGCTGGAGAGCCTGCTGC
  CCGCCCGCCCGTAAAATGGTCCCCTCGGCTGGACAGCTCGCCCTGTTCGCTCTGGGTATT
  GTGTTGGCTGCGTGCCAGGCCTTGGAGAACAGCACGTCCCCGCTGAGTGCAGACCCGCCC
  GTGGCTGCAGCAGTGGTGTCCCATTTTAATGACTGCCCAGATTCCCACACTCAGTTCTGC
  TTCCATGGAACCTGCAGGTTTTTGGTGCAGGAGGACAAGCCAGCATGTGTCTGCCATTCT
  GGGTACGTTGGTGCACGCTGTGAGCATGCGGACCTCCTGGCCGTGGTGGCTGCCAGCCAG
  AAGAAGCAGGCCATCACCGCCTTGGTGGTGGTCTCCATCGTGGCCCTGGCTGTCCTTATC
  ATCACATGTGTGCTGATACACTGCTGCCAGGTCCGAAAACACTGTGAGTGGTGCCGGGCC
  CTCATCTGCCGGCACGAGAAGCCCAGCGCCCTCCTGAAGGGAAGAACCGCTTGCTGCCAC
  TCAGAAACAGTGGTCTGAAGAGCCCAGAGGAGGAGTTTGGCCAGGTGGACTGTGGCAGAT
  CAATAAAGAAAGGCTTCTTCAGGACAGCACTGCCAGAGATGCCTGGGTGTGCCACAGACC
  TTCCTACTTGGCCTGTAATCACCTGTGCAGCCTTTTGTGGGCCTTCAAAACTCTGTCAAG
  AACTCCGTCTGCTTGGGGTTATTCAGTGTGACCTAGAGAAGAAATCAGCGGACCACGATT
  TCAAGACTTGTTAAAAAAGAACTGCAAAGAGACGGACTCCTGTTCACCTAGGTGAGGTGT
  GTGCAGCAGTTGGTGTCTGAGTCCACATGTGTGCAGTTGTCTTCTGCCAGCCATGGATTC
  CAGGCTATATATTTCTTTTTAATGGGCCACCTCCCCACAACAGAATTCTGCCCAACACAG
  GAGATTTCTATAGTTATTGTTTTCTGTCATTTGCCTACTGGGGAAGAAAGTGAAGGAGGG
  GAAACTGTTTAATATCACATGAAGACCCTAGCTTTAAGAGAAGCTGTATCCTCTAACCAC
  GAGACCCTCAACCAGCCCAACATCTTCCATGGACACATGACATTGAAGACCATCCCAAGC
  TATCGCCACCCTTGGAGATGATGTCTTATTTATTAGATGGATAATGGTTTTATTTTTAAT
  CTCTTAAGTCAATGTAAAAAGTATAAAACCCCTTCAGACTTCTAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003236 unedited
  NTTTTGTTNGCGTTCATCATTTGTNATACGACTCACTATAGGCGGCCGCGAATTCGCCAT
  TACGGCCGGGGACTCGGGCACCGCAGGTAGGGCAGGAGGCTGGAGAGCCTGCTGCCCGCC
  CGCCCGTAAAATGGTCCCCTCGGCTGGACAGCTCGCCCTGTTCGCTCTGGGTATTGTGTT
  GGCTGCGTGCCAGGCCTTGGAGAACAGCACGTCCCCGCTGAGTGCAGACCCGCCCGTGGC
  TGCAGCAGTGGTGTCCCATTTTAATGACTGCCCAGATTCCCACACTCAGTTCTGCTTCCA
  TGGAACCTGCAGGTTTTTGGTGCAGGAGGACAAGCCAGCATGTGTCTGCCATTCTGGGTA
  CGTTGGTGCACGCTGTGAGCATGCGGACCTCCTGGCCGTGGTGGCTGCCAGCCAGAAGAA
  GCAGGCCATCACCGCCTTGGTGGTGGTCTCCATCGTGGCCCTGGCTGTCCTTATCATCAC
  ATGTGTGCTGATACACTGCTGCCAGGTCCGAAAACACTGTGAGTGGTGCCGGGCCCTCAT
  CTGCCGGCACGAGAAGCCCAGCGCCCTCCTGAAGGGAAGAACCGCTTGCTGCCACTCAGA
  AACAGTGGTCTGAAGAGCCCAGAGGAGGAGTTTGGCCAGGTGGACTGTGGCAGATCAATA
  AAGAAAGGCTTCTTCAGGACAGCACTGCCAGAGATGCCTGGGTGTGCCACAGACCTTCCT
  ACTTGGCCTGTAATCACCTGTGCAGCCTTTTGTGGGCCTTCAAAACTCTGTCAAGAACTC
  CGTCTGCTTGGGGTTATTCAGTGTGACCTAGAGAAGAATCAGCGGACCACGATTTCAAGA
  CTTGTTAAAAAAGAACTGCANAGAGAC
  
• Restriction Sites: Please inquire
• ACCN: NM_003236
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003236.1, NP_003227.1
• RefSeq Size: 4119 bp
• RefSeq ORF: 483 bp
• Locus ID: 7039
• UniProt ID: P01135
• Cytogenetics: 2p13.3
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: ErbB signaling pathway, Glioma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Renal cell carcinoma
• Gene Summary: This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
  Transcript Variant: This variant (1) encodes isoform 1.