TNNT3 (NM_006757) Human Untagged Clone

CAT#: SC310590

TNNT3 (untagged)-Human troponin T type 3 (skeletal, fast) (TNNT3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TNNT3
• Synonyms: beta-TnTF; DA2B2; TNTF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006757 edited
  GAACCAGCTGGGAGGACAGTACGAGGGGCTGGTGGGCAGCCTGGGACTGGGTCACACCGG
  GGGCCTGGACAACACTGGCTCGTGGGGCTGAGGACTGAGGAGAGCCTGGCCCAGCCGTGG
  CAGTTACAAGAGGCTTAACCTGAGGGGCAGCAGAGTCTCAGGAAGAGATTCTGGGATAGG
  AAAAAGCTACCCCCGTTGCCTGGAAGACAGCTATCTGCTGGAAACCACCCACCTTCACCA
  TGTCTGACGAGGAAGTTGAACAGGTGGAGGAGCAGTACGAAGAAGAAGAGGAAGCCCAGG
  AGGAAGAGGAAGTTCAAGAAGACACCGCAGAGGAGGACGCGGAAGAGGAGAAACCGAGAC
  CCAAACTCACTGCTCCTAAGATCCCAGAAGGGGAGAAAGTGGACTTCGATGACATCCAGA
  AGAAGCGTCAGAACAAAGACCTAATGGAGCTCCAGGCCCTCATCGACAGCCACTTTGAAG
  CCCGGAAGAAGGAGGAGGAGGAGCTGGTCGCTCTCAAAGAGAGAATCGAGAAGCGCCGTG
  CAGAGAGAGCGGAGCAGCAGAGGATTCGTGCAGAGAAGGAGAGGGAGCGCCAGAACAGAC
  TGGCGGAGGAAAAGGCCAGAAGGGAGGAGGAGGATGCCAAGAGGAGGGCAGAGGACGACC
  TGAAGAAGAAGAAAGCTCTGTCTTCCATGGGAGCCAACTACAGCAGCTACCTGGCCAAGG
  CTGACCAGAAGAGAGGCAAGAAGCAGACAGCCCGGGAAATGAAGAAGAAGATTCTGGCTG
  AGAGACGCAAGCCGCTCAACATCGATCACCTTGGTGAAGACAAACTGAGGGACAAGGCCA
  AGGAGCTCTGGGAGACCCTGCACCAGCTGGAGATTGACAAGTTCGAGTTTGGGGAGAAGC
  TGAAACGCCAGAAATATGACATCATGAATGTCCGGGCCAGAGTGCAGATGCTGGCCAAGT
  TCAGCAAGAAGGCTGGGACCCCAGCCAAGGGCAAAGTCGGCGGGCGCTGGAAGTAGAGAG
  GCCAGAAAGGCCCCTCGAGGCAGAGACCCTCCGCCCTCTTGCACACCAGGGCCGCTCGTG
  GGACTCCACATCCTCCAGCCCCCACAATCCTGTCAGGGGCTCCCTGACAGTCCTGGGGGT
  GGAGAGGCCATCCCGGGGCGTCCCCCGCGTCTGTGTCCTTGCTGCCTTCATCCCCTGGGG
  CCTGTGAATAAAGCTGCAGAACCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006757 unedited
  GGGCGTTCAGATTTTGTAATACGACTTCACTATAGGGCGGCACGCGCAATTCGGCACGAG
  GGAACCAGCTGGGAGGACAGTACGAGGGGCTGGTGGGCAGCCTGGGACTGGGTCACACCG
  GGGGCCTGGACAACACTGGCTCGTGGGGCTGAGGACTGAGGAGAGCCTGGCCCAGCCGTG
  GCAGTTACAAGAGGCTTAACCTGAGGGGCAGCAGAGTCTCAGGAAGAGATTCTGGGATAG
  GAAAAAGCTACCCCCGTTGCCTGGAAGACAGCTATCTGCTGGAAACCACCCACCTTCACC
  ATGTCTGACGAGGAAGTTGAACAGGTGGAGGAGCAGTACGAAGAAGAAGAGGAAGCCCAG
  GAGGAAGAGGAAGTTCAAGAAGACACCGCAGAGGAGGACGCGGAAGAGGAGAAACCGAGA
  CCCAAACTCACTGCTCCTAAGATCCCAGAAGGGGAGAAAGTGGACTTCGATGACATCCAG
  AAGAAGCGTCAGAACAAAGACCTAATGGAGCTCCAGGCCCTCATCGACAGCCACTTTGAA
  GCCCGGAAGAAGGAGGAGGAGGAGCTGGTCGCTCTCAAAGAGAGAATCGAGAAGCGCCGT
  GCAGAGAGAGCGGAGCAGCAGAGGATTCGTGCAGAGAAGGAGAGGGAGCGCCAGAACAGA
  CTGGCGGAGGAAAAGGCCAGAAAGGAGGAGGAGGATGCCAAGAGGAGGGCAGAGGACGAC
  CTGAAGAAGAAGAAGCTCTGTCTTCCATGGGAGCCAACTACAGCAGCTACCTGGCCAAGG
  CTGACCAGAAGAGAGGCAAGAAGCAGACAGCCCGGGAAATGAAGAAGAAGATTCTGGCTG
  AGAGACGCAAGCCGCTCAACATCGATCACCTTTGGTGAAGACAAACTGAGGGACAAGGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006757 unedited
  NNGGGTTTTGACTAGNAACCGCGGCCGCATNCTAGNGATCGATTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTGGGGGTTCTGCAGCTTTATTCACAGGCCCCAAGGGATGA
  AAGCAGCAAGGACACAAACCCCGGGGACGCCCCGGGATGGCCTCTCCACCCCCAGGACTG
  TCAAGGAGCCCCTGACAGGATTGTGGGGGCTGGAGGATGTGGAGTCCCACGAACGGCCCT
  GGTGTGCAAAAAGGCGGAGGGTCTCTGCCTCGAGGGGCCTTTTTGGCCTCTCTACTTTCA
  ACGCCCGCCGACTTTGCCCTTGGCTGGGGTCCCAACCTTCTTGCTGAACTTGGCCAACAT
  CTGCACTCTGGCCCGGACATTCATGAAGTCATATTTCTGGCGTTTCAACTTCTCCCCAAA
  CTCGAACTTGTCAATCTCCAACTGAGGCAAGGTCTCCCAAAACTCCTTGGCCTTGTCCCT
  CAATTTGTCTTCACCAAAGTGATCCATGTTGAACGGCTTGCGTCTCTTAACCAGAATCTT
  CTTCTTTATTTCCCGGGCTGTCTGCTTCTTGCCTCTCTTCTGGTCAACCTTGGCCAAGTA
  ACTGCTGTAATTGGCTCCCATGGAAGACAGAACTTTCTTCTTCTTCAAGTCGTCCTCTGG
  CCTCCTCTTGGCATCCTCCTCCTCCCTTCTGGCCTTTTCCTCCGCCAGTCTGTTCTGGCG
  CTCCCTCTCCTTCTCTGCACGAATCCTCTGGTGGTCCGCTCTCTCTGGACGGCGCTTCTC
  GATTCTCTCTTTGAGAGCGAACAACTTCTTCTCCTCCTTCTTTCGGGCTTTAAAATGGCT
  GTCGATGAAGGCCTGGGAGCTCCATTAAGTCTTTGGTCTGACGCTTCTTCTTGG
  
• Restriction Sites: Please inquire
• ACCN: NM_006757
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There are 26 nucleotide differences between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 10 amino acids. There is another published reference that contains an exact match to the sequence discrepancies, which supports the existence of these polymorphisms.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006757.1, NP_006748.1
• RefSeq Size: 1000 bp
• RefSeq ORF: 777 bp
• Locus ID: 7140
• UniProt ID: P45378
• Cytogenetics: 11p15.5
• Domains: Troponin
• Gene Summary: The binding of Ca(2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca(2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction. The troponin complex has protein subunits C, I, and T. Subunit C binds Ca(2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B). [provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (1) encodes isoform 1.