PPP1A (PPP1CA) (NM_206873) Human Untagged Clone

CAT#: SC308293

PPP1CA (untagged)-Human protein phosphatase 1, catalytic subunit, alpha isozyme (PPP1CA), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP1CA
• Synonyms: PP-1A; PP1A; PP1alpha; PPP1A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_206873 edited
  GAAGGAGAGCCAGGCCGGAAGGAGGCTGCCGGAGGGCGGGAGGCAGGAGCGGGCCAGGAG
  CTGCTGGGCTGGAGCGGCGGCGCCGCCATGTCCGACAGCGAGAAGCTCAACCTGGACTCG
  ATCATCGGGCGCCTGCTGGAAGGTGACATACACGGCCAGTACTACGACCTTCTGCGACTA
  TTTGAGTATGGCGGTTTCCCTCCCGAGAGCAACTACCTCTTTCTGGGGGACTATGTGGAC
  AGGGGCAAGCAGTCCTTGGAGACCATCTGCCTGCTGCTGGCCTATAAGATCAAGTACCCC
  GAGAACTTCTTCCTGCTCCGTGGGAACCACGAGTGTGCCAGCATCAACCGCATCTATGGT
  TTCTACGATGAGTGCAAGAGACGCTACAACATCAAACTGTGGAAAACCTTCACTGACTGC
  TTCAACTGCCTGCCCATCGCGGCCATAGTGGACGAAAAGATCTTCTGCTGCCACGGAGGC
  CTGTCCCCGGACCTGCAGTCTATGGAGCAGATTCGGCGGATCATGCGGCCCACAGATGTG
  CCTGACCAGGGCCTGCTGTGTGACCTGCTGTGGTCTGACCCTGACAAGGACGTGCAGGGC
  TGGGGCGAGAACGACCGTGGCGTCTCTTTTACCTTTGGAGCCGAGGTGGTGGCCAAGTTC
  CTCCACAAGCACGACTTGGACCTCATCTGCCGAGCACACCAGGTGGTAGAAGACGGCTAC
  GAGTTCTTTGCCAAGCGGCAGCTGGTGACACTTTTCTCAGCTCCCAACTACTGTGGCGAG
  TTTGACAATGCTGGCGCCATGATGAGTGTGGACGAGACCCTCATGTGCTCTTTCCAGATC
  CTCAAGCCCGCCGACAAGAACAAGGGGAAGTACGGGCAGTTCAGTGGCCTGAACCCTGGA
  GGCCGACCCATCACCCCACCCCGCAATTCCGCCAAAGCCAAGAAATAGCCCCCGCACACC
  ACCCTGTGCCCCAGATGATGGATTGATTGTACAGAAATCATGCTGCCATGCTGGGGGGGG
  GTCACCCCGACCCCTCAGGCCCACCTGTCACGGGGAACATGGAGCCTTGGTGTATTTTTC
  TTTTCTTTTTTTAATGAATCAATAGCAGCGTCCAGTCCCCCAGGGCTGCTTCCTGCCTGC
  ACCTGCGGTGACTGTGAGCAGGATCCTGGGGCCGAGGCTGCAGCTCAGGGCAACGGCAGG
  CCAGGTCGTGGGTCTCCAGCCGTGCTTGGCCTCAGGGCTGGCAGCCGGATCCTGGGGCAA
  CCCATCTGGTCTCTTGAATAAAGGTCAAAGCTGGATTCTCAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_206873 unedited
  GTCACAATTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACCAGGGAAGAGAGCC
  AGGCCGGAAGGAGGCTGCCGGAGGGCGGGAGGCAGGAGCGGGCCAGGAGCTGCTGGGCTG
  GAGCGGCGGCGCCGCCATGTCCGACAGCGAGAAGCTCAACCTGGACTCGATCATCGGGCG
  CCTGCTGGAAGGTGACATACACGGCCAGTACTACGACCTTCTGCGACTATTTGAGTATGG
  CGGTTTCCCTCCCGAGAGCAACTACCTCTTTCTGGGGGACTATGTGGACAGGGGCAAGCA
  GTCCTTGGAGACCATCTGCCTGCTGCTGGCCTATAAGATCAAGTACCCCGAGAACTTCTT
  CCTGCTCCGTGGGAACCACGAGTGTGCCAGCATCAACCGCATCTATGGTTTCTACGATGA
  GTGCAAGAGACGCTACAACATCAAACTGTGGAAAACCTTCACTGACTGCTTCAACTGCCT
  GCCCATCGCGGCCATAGTGGACGAAAAGATCTTCTGCTGCCACGGAGGCCTGTCCCCGGA
  CCTGCAGTCTATGGAGCAGATTCGGCGGATCATGCGGCCCACAGATGTGCCTGACCAGGG
  CCTGCTGTGTGACCTGCTGTGGTCTGACCCTGACAGGACGTGCANGGCTGGGGCGAGAAC
  GACCGTGGCGTCTCTTTTACCTTGGAGCCGAGGTGGTGGCCAAGTTCCTCCACAGCACGA
  CTTGGACTCATCTGCCGAGCACACCNAGTGGTANAAGACGGCTACNAGTTCTTTGCCAAG
  NCGCAGCTGGTGACACTTTTCTCAGCTCCAACTACTGTGGCGAGTTTGACATGCTGGCGC
  CTGATGAGTGTGGACGAGACCCTATGTGCTCTTTCCATTC
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_206873 unedited
  TTTGCCCTACCGGCCATATGGTAGAAAGATCTTCTGCTGCCACGNAGCCTGTCCCCGGAC
  CTGCAGTCTATGGAGCAGATTCGGCGGATCATGCGGCCCACAGATGTGCCTGACCAGGGC
  CTGCTGTGTGACCTGCTGTGGTCTGACCCTGACAAGGACGTGCAGGGCTGGGGCGAGAAC
  GACCGTGGCGTCTCTTTTACCTTTGGAGCCGAGGTGGTGGCCAAGTTCCTCCACAAGCAC
  GACTTGGACCTCATCTGCCGAGCACACCAGGTGGTAGAAGACGGCTACGAGTTCTTTGCC
  AAGCGGCAGCTGGTGACACTTTTCTCAGCTCCCAACTACTGTGGCGAGTTTGACAATGCT
  GGCGCCATGATGAGTGTGGACGAGACCCTCATGTGCTCTTTCCAGATCCTCAAGCCCGCC
  GACAAGAACAAGGGGAAGTACGGGCAGTTCAGTGGCCTGAACCCTGGAGGCCGACCCATC
  ACCCCACCCCGCAATTCCGCCAAAGCCAAGAAATAGCCCCCGCACACCACCCTGTGCCCC
  AGATGATGGATTGATTGTACAGAAATCATGCTGCCATGCTGGGGGGGGGGTCACCCCGAC
  CCCTCAGGCCCACCTGTCACGGNGAACATGGAGCCTTGGTGTATTTTTCTTTTCTTTTTT
  TAATGAATCAATAGCAGCGTCCAGTCCCCCAGGGCTGCTTCCTGCCTGCACCCTGCGTGA
  CTGTGAGCAGGATCCTGGGGCCCGAGCTGCAGCTCAAGGCAACCGCCAGCCCAGTCGTGG
  GTCCTCAGCCGTGCTTGACCTCAAGGCTGCCAGCCGGATTCTGGGGGCAACCCATCTGGG
  TCTCTTGAATAAAGTCAAAAGCTGAATCTTAAAAAAAAAAANAAAAAAAAACCCGAACCT
  TTAAATTGCGGCCCCA
  
• Restriction Sites: Please inquire
• ACCN: NM_206873
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_206873.1, NP_996756.1
• RefSeq Size: 1356 bp
• RefSeq ORF: 861 bp
• Locus ID: 5499
• UniProt ID: P62136
• Cytogenetics: 11q13.2
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Focal adhesion, Insulin signaling pathway, Long-term potentiation, Oocyte meiosis, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
• Gene Summary: The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). This broadly expressed gene encodes the alpha subunit of the PP1 complex that associates with over 200 regulatory proteins to form holoenzymes which dephosphorylate their biological targets with high specificity. PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies suggest that PP1 is an important regulator of cardiac function and that PP1 deregulation is implicated in diabetes and multiple types of cancer. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2020]
  Transcript Variant: This variant (2) lacks an in-frame coding exon compared to variant 1. The resulting isoform (2) lacks an internal region, as compared to isoform 1.