Collagen II (COL2A1) (NM_033150) Human Untagged Clone

CAT#: SC305574

COL2A1 (untagged)-Human collagen, type II, alpha 1 (COL2A1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COL2A1
• Synonyms: ANFH; AOM; COL11A3; SEDC; STL1
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033150, the custom clone sequence may differ by one or more nucleotides
  ATGATTCGCCTCGGGGCTCCCCAGACGCTGGTGCTGCTGACGCTGCTCGTCGCCGCTGTC
  CTTCGGTGTCAGGGCCAGGATGTCCGGCAACCAGGACCAAAGGGACAGAAAGGAGAACCT
  GGAGACATCAAGGATATTGTAGGACCCAAAGGACCTCCTGGGCCTCAGGGACCTGCAGGG
  GAACAAGGACCCAGAGGGGATCGTGGTGACAAAGGTGAAAAAGGTGCCCCTGGACCTCGT
  GGCAGAGATGGAGAACCTGGGACCCCTGGAAATCCTGGCCCCCCTGGTCCTCCCGGCCCC
  CCTGGTCCCCCTGGTCTTGGTGGAAACTTTGCTGCCCAGATGGCTGGAGGATTTGATGAA
  AAGGCTGGTGGCGCCCAGTTGGGAGTAATGCAAGGACCAATGGGCCCCATGGGACCTCGA
  GGACCTCCAGGCCCTGCAGGTGCTCCTGGGCCTCAAGGATTTCAAGGCAATCCTGGTGAA
  CCTGGTGAACCTGGTGTCTCTGGTCCCATGGGTCCCCGTGGTCCTCCTGGTCCCCCTGGA
  AAGCCTGGTGATGATGGTGAAGCTGGAAAACCTGGAAAAGCTGGTGAAAGGGGTCCGCCT
  GGTCCTCAGGGTGCTCGTGGTTTCCCAGGAACCCCAGGCCTTCCTGGTGTCAAAGGTCAC
  AGAGGTTATCCAGGCCTGGACGGTGCTAAGGGAGAGGCGGGTGCTCCTGGTGTGAAGGGT
  GAGAGTGGTTCCCCGGGTGAGAACGGATCTCCGGGCCCAATGGGTCCTCGTGGCCTGCCT
  GGTGAAAGAGGACGGACTGGCCCTGCTGGCGCTGCGGGTGCCCGAGGCAACGATGGTCAG
  CCAGGCCCCGCAGGGCCTCCGGGTCCTGTCGGTCCTGCTGGTGGTCCTGGCTTCCCTGGT
  GCTCCTGGAGCCAAGGGTGAAGCCGGCCCCACTGGTGCCCGTGGTCCTGAAGGTGCTCAA
  GGTCCTCGCGGTGAACCTGGTACTCCTGGGTCCCCTGGGCCTGCTGGTGCCTCCGGTAAC
  CCTGGAACAGATGGAATTCCTGGAGCCAAAGGATCTGCTGGTGCTCCTGGCATTGCTGGT
  GCTCCTGGCTTCCCTGGGCCACGGGGCCCTCCTGGCCCTCAAGGTGCAACTGGTCCTCTG
  GGCCCGAAAGGTCAGACGGGTGAACCTGGTATTGCTGGCTTCAAAGGTGAACAAGGCCCC
  AAGGGAGAACCTGGCCCTGCTGGCCCCCAGGGAGCCCCTGGACCCGCTGGTGAAGAAGGC
  AAGAGAGGTGCCCGTGGAGAGCCTGGTGGCGTTGGGCCCATCGGTCCCCCTGGAGAAAGA
  GGTGCTCCCGGCAACCGCGGTTTCCCAGGTCAAGATGGTCTGGCAGGTCCCAAGGGAGCC
  CCTGGAGAGCGAGGGCCCAGTGGTCTTGCTGGCCCCAAGGGAGCCAACGGTGACCCTGGC
  CGTCCTGGAGAACCTGGCCTTCCTGGAGCCCGGGGTCTCACTGGCCGCCCTGGTGATGCT
  GGTCCTCAAGGCAAAGTTGGCCCTTCTGGAGCCCCTGGTGAAGATGGTCGTCCTGGACCT
  CCAGGTCCTCAGGGGGCTCGTGGGCAGCCTGGTGTCATGGGTTTCCCTGGCCCCAAAGGT
  GCCAACGGTGAGCCTGGCAAAGCTGGTGAGAAGGGACTGCCTGGTGCTCCTGGTCTGAGG
  GGTCTTCCTGGCAAAGATGGTGAGACAGGTGCTGCAGGACCCCCTGGCCCTGCTGGACCT
  GCTGGTGAACGAGGCGAGCAGGGTGCTCCTGGGCCATCTGGGTTCCAGGGACTTCCTGGC
  CCTCCTGGTCCCCCAGGTGAAGGTGGAAAACCAGGTGACCAGGGTGTTCCCGGTGAAGCT
  GGAGCCCCTGGCCTCGTGGGTCCCAGGGGTGAACGAGGTTTCCCAGGTGAACGTGGCTCT
  CCCGGTGCCCAGGGCCTCCAGGGTCCCCGTGGCCTCCCCGGCACTCCTGGCACTGATGGT
  CCCAAAGGTGCATCTGGCCCAGCAGGCCCCCCTGGGGCTCAGGGCCCTCCAGGTCTTCAG
  GGAATGCCTGGCGAGAGGGGAGCAGCTGGTATCGCTGGGCCCAAAGGCGACAGGGGTGAC
  GTTGGTGAGAAAGGCCCTGAGGGAGCCCCTGGAAAGGATGGTGGACGAGGCCTGACAGGT
  CCCATTGGCCCCCCTGGCCCAGCTGGTGCTAATGGCGAGAAGGGAGAAGTTGGACCTCCT
  GGTCCTGCAGGAAGTGCTGGTGCTCGTGGCGCTCCGGGTGAACGTGGAGAGACTGGGCCC
  CCCGGACCAGCGGGATTTGCTGGGCCTCCTGGTGCTGATGGCCAGCCTGGGGCCAAGGGT
  GAGCAAGGAGAGGCCGGCCAGAAAGGCGATGCTGGTGCCCCTGGTCCTCAGGGCCCCTCT
  GGAGCACCTGGGCCTCAGGGTCCTACTGGAGTGACTGGTCCTAAAGGAGCCCGAGGTGCC
  CAAGGCCCCCCGGGAGCCACTGGATTCCCTGGAGCTGCTGGCCGCGTTGGACCCCCAGGC
  TCCAATGGCAACCCTGGACCCCCTGGTCCCCCTGGTCCTTCTGGAAAAGATGGTCCCAAA
  GGTGCTCGAGGAGACAGCGGCCCCCCTGGCCGAGCTGGTGAACCCGGCCTCCAAGGTCCT
  GCTGGACCCCCTGGCGAGAAGGGAGAGCCTGGAGATGACGGTCCCTCTGGTGCCGAAGGT
  CCACCAGGTCCCCAGGGTCTGGCTGGTCAGAGAGGCATCGTCGGTCTGCCTGGGCAACGT
  GGTGAGAGAGGATTCCCTGGCTTGCCTGGCCCGTCGGGTGAGCCCGGCAAGCAGGGTGCT
  CCTGGAGCATCTGGAGACAGAGGTCCTCCTGGCCCCGTGGGTCCTCCTGGCCTGACGGGT
  CCTGCAGGTGAACCTGGACGAGAGGGAAGCCCCGGTGCTGATGGCCCCCCTGGCAGAGAT
  GGCGCTGCTGGAGTCAAGGGTGATCGTGGTGAGACTGGTGCTGTGGGAGCTCCTGGAGCC
  CCTGGGCCCCCTGGCTCCCCTGGCCCCGCTGGTCCAACTGGCAAGCAAGGAGACAGAGGA
  GAAGCTGGTGCACAAGGCCCCATGGGACCCTCAGGACCAGCTGGAGCCCGGGGAATCCAG
  GGTCCTCAAGGCCCCAGAGGTGACAAAGGAGAGGCTGGAGAGCCTGGCGAGAGAGGCCTG
  AAGGGACACCGTGGCTTCACTGGTCTGCAGGGTCTGCCCGGCCCTCCTGGTCCTTCTGGA
  GACCAAGGTGCTTCTGGTCCTGCTGGTCCTTCTGGCCCTAGAGGTCCTCCTGGCCCCGTC
  GGTCCCTCTGGCAAAGATGGTGCTAATGGAATCCCTGGCCCCATTGGGCCTCCTGGTCCC
  CGTGGACGATCAGGCGAAACCGGCCCTGCTGGTCCTCCTGGAAATCCTGGACCCCCTGGT
  CCTCCAGGTCCCCCTGGCCCTGGCATCGACATGTCCGCCTTTGCTGGCTTAGGCCCGAGA
  GAGAAGGGCCCCGACCCCCTGCAGTACATGCGGGCCGACCAGGCAGCCGGTGGCCTGAGA
  CAGCATGACGCCGAGGTGGATGCCACACTCAAGTCCCTCAACAACCAGATTGAGAGCATC
  CGCAGCCCCGAGGGCTCCCGCAAGAACCCTGCTCGCACCTGCAGAGACCTGAAACTCTGC
  CACCCTGAGTGGAAGAGTGGAGACTACTGGATTGACCCCAACCAAGGCTGCACCTTGGAC
  GCCATGAAGGTTTTCTGCAACATGGAGACTGGCGAGACTTGCGTCTACCCCAATCCAGCA
  AACGTTCCCAAGAAGAACTGGTGGAGCAGCAAGAGCAAGGAGAAGAAACACATCTGGTTT
  GGAGAAACCATCAATGGTGGCTTCCATTTCAGCTATGGAGATGACAATCTGGCTCCCAAC
  ACTGCCAACGTCCAGATGACCTTCCTACGCCTGCTGTCCACGGAAGGCTCCCAGAACATC
  ACCTACCACTGCAAGAACAGCATTGCCTATCTGGACGAAGCAGCTGGCAACCTCAAGAAG
  GCCCTGCTCATCCAGGGCTCCAATGACGTGGAGATCCGGGCAGAGGGCAATAGCAGGTTC
  ACGTACACTGCCCTGAAGGATGGCTGCACGAAACATACCGGTAAGTGGGGCAAGACTGTT
  ATCGAGTACCGGTCACAGAAGACCTCACGCCTCCCCATCATTGACATTGCACCCATGGAC
  ATAGGAGGGCCCGAGCAGGAATTCGGTGTGGACATAGGGCCGGTCTGCTTCTTGTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_033150
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033150.1, NP_149162.1
• RefSeq Size: 4853 bp
• RefSeq ORF: 4257 bp
• Locus ID: 1280
• UniProt ID: P02458
• Cytogenetics: 12q13.11
• Protein Pathways: ECM-receptor interaction, Focal adhesion
• Gene Summary: This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in a shorter protein. The resulting protein (isoform 2) lacks the von Willebrand factor type C (VWC) domain, compared to isoform 1.