FANCD2 (NM_033084) Human Untagged Clone

CAT#: SC305556

FANCD2 (untagged)-Human Fanconi anemia, complementation group D2 (FANCD2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FANCD2
• Synonyms: FA-D2; FA4; FACD; FAD; FAD2; FANCD
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033084, the custom clone sequence may differ by one or more nucleotides
  ATGGTTTCCAAAAGAAGACTGTCAAAATCTGAGGATAAAGAGAGCCTGACAGAAGATGCC
  TCCAAAACCAGGAAGCAACCACTTTCCAAAAAGACAAAGAAATCTCATATTGCTAATGAA
  GTTGAAGAAAATGACAGCATCTTTGTAAAGCTTCTTAAGATATCAGGAATTATTCTTAAA
  ACGGGAGAGAGTCAGAATCAACTAGCTGTGGATCAAATAGCTTTCCAAAAGAAGCTCTTT
  CAGACCCTGAGGAGACACCCTTCCTATCCCAAAATAATAGAAGAATTTGTTAGTGGCCTG
  GAGTCTTACATTGAGGATGAAGACAGTTTCAGGAACTGCCTTTTGTCTTGTGAGCGTCTG
  CAGGATGAGGAAGCCAGTATGGGTGCATCTTATTCTAAGAGTCTCATCAAACTGCTTCTG
  GGGATTGACATACTGCAGCCTGCCATTATCAAAACCTTATTTGAGAAGTTGCCAGAATAT
  TTTTTTGAAAACAAGAACAGTGATGAAATCAACATACCTCGACTCATTGTCAGTCAACTA
  AAATGGCTTGACAGAGTTGTGGATGGCAAGGACCTCACCACCAAGATCATGCAGCTGATC
  AGTATTGCTCCAGAGAACCTGCAGCATGACATCATCACCAGCCTACCTGAGATCCTAGGG
  GATTCCCAGCACGCTGATGTGGGGAAAGAACTCAGTGACCTACTGATAGAGAATACTTCA
  CTCACTGTCCCAATCCTGGATGTCCTTTCAAGCCTCCGACTTGACCCAAACTTCCTATTG
  AAGGTTCGCCAGTTGGTGATGGATAAGTTGTCGTCTATTAGATTGGAGGATTTACCTGTG
  ATAATAAAGTTCATTCTTCATTCCGTAACAGCCATGGATACACTTGAGGTAATTTCTGAG
  CTTCGGGAGAAGTTGGATCTGCAGCATTGTGTTTTGCCATCACGGTTACAGGCTTCCCAA
  GTAAAGTTGAAAAGTAAAGGACGAGCAAGTTCCTCAGGAAATCAAGAAAGCAGCGGTCAG
  AGCTGTATTATTCTCCTCTTTGATGTAATAAAGTCAGCTATTAGATATGAGAAAACCATT
  TCAGAAGCCTGGATTAAGGCAATTGAAAACACTGCCTCAGTATCTGAACACAAGGTGTTT
  GACCTGGTGATGCTTTTCATCATCTATAGCACCAATACTCAGACAAAGAAGTACATTGAC
  AGGGTGCTAAGAAATAAGATTCGATCAGGCTGCATTCAAGAACAGCTGCTCCAGAGTACA
  TTCTCTGTTCATTACTTAGTTCTTAAGGATATGTGTTCATCCATTCTGTCGCTGGCTCAG
  AGTTTGCTTCACTCTCTAGACCAGAGTATAATTTCATTTGGCAGTCTCCTATACAAATAT
  GCATTTAAGTTTTTTGACACGTACTGCCAGCAGGAAGTGGTTGGTGCCTTAGTGACCCAT
  ATCTGCAGTGGGAATGAAGCTGAAGTTGATACTGCCTTAGATGTCCTTCTAGAGTTGGTA
  GTGTTAAACCCATCTGCTATGATGATGAATGCTGTCTTTGTAAAGGGCATTTTAGATTAT
  CTGGATAACATATCCCCTCAGCAAATACGAAAACTCTTCTATGTTCTCAGCACACTGGCA
  TTTAGCAAACAGAATGAAGCCAGCAGCCACATCCAGGATGACATGCACTTGGTGATAAGA
  AAGCAGCTCTCTAGCACCGTATTCAAGTACAAGCTCATTGGGATTATTGGTGCTGTGACC
  ATGGCTGGCATCATGGCGGCAGACAGAAGTGAATCACCTAGTTTGACCCAAGAGAGAGCC
  AACCTGAGCGATGAGCAGTGCACACAGGTGACCTCCTTGTTGCAGTTGGTTCATTCCTGC
  AGTGAGCAGTCTCCTCAGGCCTCTGCACTTTACTATGATGAATTTGCCAACCTGATCCAA
  CATGAAAAGCTGGATCCAAAAGCCCTGGAATGGGTTGGGCATACCATCTGTAATGATTTC
  CAGGATGCCTTCGTAGTGGACTCCTGTGTTGTTCCGGAAGGTGACTTTCCATTTCCTGTG
  AAAGCACTGTACGGACTGGAAGAATACGACACTCAGGATGGGATTGCCATAAACCTCCTG
  CCGCTGCTGTTTTCTCAGGACTTTGCAAAAGATGGGGGTCCGGTGACCTCACAGGAATCA
  GGCCAAAAATTGGTGTCTCCGCTGTGCCTGGCTCCGTATTTCCGGTTACTGAGACTTTGT
  GTGGAGAGACAGCATAACGGAAACTTGGAGGAGATTGATGGTCTACTAGATTGTCCTATA
  TTCCTAACTGACCTGGAGCCTGGAGAGAAGTTGGAGTCCATGTCTGCTAAAGAGCGTTCA
  TTCATGTGTTCTCTCATATTTCTTACTCTCAACTGGTTCCGAGAGATTGTAAATGCCTTC
  TGCCAGGAAACATCACCTGAGATGAAGGGGAAGGTGCTCACTCGGTTAAAGCACATTGTA
  GAATTGCAAATAATCCTGGAAAAGTACTTGGCAGTCACCCCAGACTATGTCCCTCCTCTT
  GGAAACTTTGATGTGGAAACTTTAGATATAACACCTCATACTGTTACTGCTATTTCAGCA
  AAAATCAGAAAGAAAGGAAAAATAGAAAGGAAACAAAAAACAGATGGCAGCAAGACATCC
  TCCTCTGACACACTTTCAGAAGAGAAAAATTCAGAATGTGACCCTACGCCATCTCATAGA
  GGCCAGCTAAACAAGGAGTTCACAGGGAAGGAAGAAAAGACATCATTGTTACTACATAAT
  TCCCATGCTTTTTTCCGAGAGCTGGACATTGAGGTCTTCTCTATTCTACATTGTGGACTT
  GTGACGAAGTTCATCTTAGATACTGAAATGCACACTGAAGCTACAGAAGTTGTGCAACTT
  GGGCCCCCTGAGCTGCTTTTCTTGCTGGAAGATCTCTCCCAGAAGCTGGAGAGTATGCTG
  ACACCTCCTATTGCCAGGAGAGTCCCCTTTCTCAAGAACAAAGGAAGCCGGAATATTGGA
  TTCTCACATCTCCAACAGAGATCTGCCCAAGAAATTGTTCATTGTGTTTTTCAACTGCTG
  ACCCCAATGTGTAACCACCTGGAGAACATTCACAACTATTTTCAGTGTTTAGCTGCTGAG
  AATCACGGTGTAGTTGATGGACCAGGAGTGAAAGTTCAGGAGTACCACATAATGTCTTCC
  TGCTATCAGAGGCTGCTGCAGATTTTTCATGGGCTTTTTGCTTGGAGTGGATTTTCTCAA
  CCTGAAAATCAGAATTTACTGTATTCAGCCCTCCATGTCCTTAGTAGCCGACTGAAACAG
  GGAGAACACAGCCAGCCTTTGGAGGAACTACTCAGCCAGAGCGTCCATTACTTGCAGAAT
  TTCCATCAAAGCATTCCCAGTTTCCAGTGTGCTCTTTATCTCATCAGACTTTTGATGGTT
  ATTTTGGAGAAATCAACAGCTTCTGCTCAGAACAAAGAAAAAATTGCTTCCCTTGCCAGA
  CAATTCCTCTGTCGGGTGTGGCCAAGTGGGGATAAAGAGAAGAGCAACATCTCTAATGAC
  CAGCTCCATGCTCTGCTCTGTATCTACCTGGAGCACACAGAGAGCATTCTGAAGGCCATA
  GAGGAGATTGCTGGTGTTGGTGTCCCAGAACTGATCAACTCTCCTAAAGATGCATCTTCC
  TCCACATTCCCTACACTGACCAGGCATACTTTTGTTGTTTTCTTCCGTGTGATGATGGCT
  GAACTAGAGAAGACGGTGAAAAAAATTGAGCCTGGCACAGCAGCAGACTCGCAGCAGATT
  CATGAAGAGAAACTCCTCTACTGGAACATGGCTGTTCGAGACTTCAGTATCCTCATCAAC
  TTGATAAAGGTATTTGATAGTCATCCTGTTCTGCATGTATGTTTGAAGTATGGGCGTCTC
  TTTGTGGAAGCATTTCTGAAGCAATGTATGCCGCTCCTAGACTTCAGTTTTAGAAAACAC
  CGGGAAGATGTTCTGAGCTTACTGGAAACCTTCCAGTTGGACACAAGGCTGCTTCATCAC
  CTGTGTGGGCATTCCAAGATTCACCAGGACACGAGACTCACCCAACATGTGCCTCTGCTC
  AAAAAGACCCTGGAACTTTTAGTTTGCAGAGTCAAAGCTATGCTCACTCTCAACAATTGT
  AGAGAGGCTTTCTGGCTGGGCAATCTAAAAAACCGGGACTTGCAGGGTGAAGAGATTAAG
  TCCCAAAATTCCCAGGAGAGCACAGCAGATGAGAGTGAGGATGACATGTCATCCCAGGCC
  TCCAAGAGCAAAGCCACTGAGGTATCTCTACAAAACCCACCAGAGTCTGGCACTGATGGT
  TGCATTTTGTTAATTGTTCTAAGTTGGTGGAGCAGAACTTTGCCTACTTATGTTTATTGT
  CAAATGCTTCTATGCCCATTTCCATTCCCTCCATAA
  
• Restriction Sites: Please inquire
• ACCN: NM_033084
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033084.3, NP_149075.2
• RefSeq Size: 5204 bp
• RefSeq ORF: 4416 bp
• Locus ID: 2177
• UniProt ID: Q9BXW9
• Cytogenetics: 3p25.3
• Protein Families: Druggable Genome
• Gene Summary: The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a).