GALNT8 (NM_017417) Human Untagged Clone

CAT#: SC304459

GALNT8 (untagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 8 (GalNAc-T8) (GALNT8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GALNT8
• Synonyms: GALNAC-T8
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_017417 edited
  ATGATGTTTTGGAGGAAACTCCCCAAAGCCCTCTTCATTGGGCTGACTCTGGCCATTGCT
  GTCAATCTCCTTCTGGTATTTTCTAGCAAGGGGACTTTACAAAACCTGTTTACGGGTGGT
  CTCCACAGGGAGCTTCCTTTACATCTGAATAAACGCTACGGGGCAGTGATAAAGAGACTC
  TCCCACTTGGAGGTGGAATTGCAGGATCTGAAAGAAAGTATGAAATTAGCTCTGAGGCAA
  CAAGAAAATGTGAACAGCACACTGAAGAGGGCGAAAGATGAAGTACGCCCTCTTCTAAAG
  GCAATGGAAACCAAGGTGAATGAGACAAAGAAGCACAAAACCCAAATGAAACTCTTCCCA
  CACTCACAGCTTTTCAGGCAATGGGGCGAGGATCTTTCTGAGGCCCAGCAGAAGGCGGCC
  CAGGACCTCTTCCGGAAGTTTGGTTACAACGCGTACCTCAGCAACCAGCTGCCTCTCAAT
  CGCACCATCCCCGACACGCGAGACTACAGATGTCTTCGGAAGACATATCCTTCCCAACTC
  CCATCCCTCAGTGTCATTCTCATATTCGTGAATGAAGCTCTGTCCATTATACAACGGGCC
  ATCACCAGTATCATCAACCGGACGCCCTCTCGATTGTTGAAGGAAATCATCTTGGTGGAT
  GATTTCAGCTCAAATGGAGAACTAAAGGTACACTTGGATGAGAAGATTAAGCTTTACAAC
  CAGAAGTATCCAGGACTACTGAAAATAATACGGCATCCTGAAAGGAAAGGTCTTGCTCAA
  GCCCGCAACACTGGCTGGGAAGCTGCCACAGCAGACGTGGTCGCCATCTTGGATGCTCAC
  ATTGAAGTCAATGTTGGGTGGGCAGAGCCAATCTTGGCTCGGATTCAGGAGGACCGCACT
  GTGATTGTGTCTCCTGTGTTTGACAACATTCGTTTTGACACCTTCAAACTGGATAAGTAT
  GAACTGGCAGTTGATGGGTTTAACTGGGAACTCTGGTGCCGCTACGATGCACTGCCACAA
  GCCTGGATTGATCTGCATGATGTCACTGCCCCAGTGAAGAGTCCTTCAATCATGGGCATC
  CTGGCTGCTAACAGGCACTTCCTGGGAGAGATCGGGTCTCTGGATGGTGGAATGCTCATC
  TATGGAGGAGAGAACGTGGAGCTTAGCCTGAGGGTGTGGCAGTGTGGAGGGAAGGTCGAG
  ATTTTGCCCTGTTCCCGGATTGCCCACCTAGAGAGACACCACAAGCCCTACGCCTTGGAT
  CTCACCGCTGCCTTGAAGCGCAATGCTCTGCGAGTGGCCGAAATCTGGATGGATGAGCAC
  AAACACATGGTCTACTTGGCCTGGAACATACCTCTCCAGAACTCTGGAATAGATTTTGGA
  GACGTTTCTTCCAGAATGGCACTCCGGGAAAAACTGAAATGTAAAACTTTTGACTGGTAC
  CTGAAAAATGTTTATCCACTCTTGAAGCCACTCCACACCATCGTGGGCTATGGAAGAATG
  AAAAACCTATTGGATGAAAATGTCTGCTTGGATCAGGGACCCGTTCCAGGCAACACCCCC
  ATCATGTATTACTGCCATGAATTCAGCTCACAGAATGTCTACTATCACCTAACTGGGGAG
  CTCTATGTGGGACAACTGATTGCAGAGGCCAGTGCTAGTGATCGCTGCCTGACAGACCCT
  GGCAAGGCGGAGAAGCCCACCTTAGAACCATGCTCCAAGGCAGCTAAGAATAGACTGCAT
  ATATATTGGGATTTTAAACCGGGAGGAGCTGTCATAAACAGAGATACCAAGCGGTGTCTG
  GAGATGAAGAAGGATCTTTTGGGTAGCCACGTGCTTGTGCTCCAGACCTGTAGCACGCAA
  GTGTGGGAAATCCAGCACACTGTCAGAGACTGGGGTCAGACCAACAGCCAGTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_017417
• Insert Size: 1900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017417.1, NP_059113.1
• RefSeq Size: 2135 bp
• RefSeq ORF: 1914 bp
• Locus ID: 26290
• UniProt ID: Q9NY28
• Cytogenetics: 12p13.32
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• Gene Summary: This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. [provided by RefSeq, Jul 2008]