DBC2 (RHOBTB2) (NM_015178) Human Untagged Clone

CAT#: SC304248

RHOBTB2 (untagged)-Human Rho-related BTB domain containing 2 (RHOBTB2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DBC2
• Synonyms: DBC2; DEE64; EIEE64; p83
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015178, the custom clone sequence may differ by one or more nucleotides
  ATGGATTCTGACATGGATTATGAAAGGCCAAACGTAGAGACCATCAAGTGCGTTGTGGTG
  GGGGACAACGCCGTGGGTAAGACCAGGCTCATCTGTGCCCGCGCTTGCAATGCCACCCTC
  ACCCAGTACCAGCTGCTGGCCACGCATGTGCCCACAGTATGGGCCATCGACCAATATCGT
  GTGTGCCAGGAGGTGCTGGAACGCTCCCGAGACGTGGTAGATGATGTCAGCGTCTCTCTG
  CGCCTCTGGGACACCTTTGGAGACCACCACAAAGACCGTCGCTTTGCTTATGGGAGATCT
  GATGTGGTGGTTCTGTGCTTCTCCATTGCCAACCCCAATTCCCTCCACCATGTCAAGACC
  ATGTGGTACCCAGAAATCAAGCACTTCTGCCCCCGAGCACCTGTCATCTTGGTGGGCTGC
  CAGTTGGACCTGCGCTACGCTGACCTGGAGGCTGTCAACAGGGCTAGGCGACCCTTGGCT
  AGGCCCATCAAACCTAATGAAATCCTGCCCCCAGAGAAGGGTCGGGAGGTGGCCAAGGAG
  CTGGGCATCCCCTACTATGAGACCAGCGTGGTGGCCCAGTTCGGCATCAAGGACGTCTTT
  GACAACGCCATCCGAGCTGCACTCATCTCCCGCCGCCACCTGCAGTTCTGGAAGTCCCAC
  CTCCGCAATGTGCAGCGGCCTCTGCTGCAGGCACCCTTCCTACCCCCCAAGCCACCGCCC
  CCGATCATCGTGGTGCCCGACCCTCCCTCCAGCAGCGAGGAGTGCCCCGCCCACCTCCTG
  GAGGACCCGCTCTGCGCGGACGTCATCCTGGTGCTGCAGGAGCGGGTGCGCATCTTTGCC
  CACAAGATCTACCTCTCCACCTCTTCCTCCAAGTTCTATGACCTGTTCCTCATGGACCTG
  AGTGAGGGGGAGCTGGGGGGCCCCTCGGAGCCAGGGGGCACCCACCCAGAGGACCACCAG
  GGCCACTCTGATCAACACCACCACCATCACCACCACCACCATGGGCGAGACTTCCTGCTC
  CGAGCAGCCAGCTTTGACGTGTGCGAGAGCGTGGATGAGGCTGGGGGCTCCGGTCCTGCT
  GGCCTCCGTGCTTCCACCAGCGACGGGATCTTACGGGGCAACGGAACAGGGTACCTACCG
  GGCAGGGGTCGTGTGCTGTCTTCCTGGAGCCGAGCTTTTGTGAGCATCCAGGAAGAGATG
  GCAGAAGATCCTCTCACCTACAAATCCCGGCTGATGGTGGTGGTGAAGATGGACAGTTCC
  ATCCAGCCGGGGCCCTTCCGGGCTGTCCTCAAGTACCTGTACACGGGGGAGCTAGATGAG
  AACGAGCGTGACCTCATGCACATTGCCCACATTGCTGAGCTGCTCGAGGTCTTTGATCTG
  CGCATGATGGTGGCCAACATTCTCAACAATGAGGCCTTCATGAACCAGGAGATCACCAAG
  GCCTTCCACGTCCGCCGGACCAACCGGGTTAAGGAGTGCTTGGCAAAAGGCACCTTCTCA
  GATGTGACCTTCATCCTGGATGATGGCACCATCAGCGCCCACAAGCCCCTGTTGATTTCC
  AGCTGTGACTGGATGGCTGCCATGTTTGGGGGGCCATTTGTGGAGAGCTCCACCCGGGAG
  GTGGTGTTTCCCTACACAAGCAAGAGCTGCATGCGGGCCGTGCTGGAATACCTCTACACC
  GGCATGTTCACCTCCAGCCCCGACCTGGATGACATGAAGCTCATCATTCTAGCCAACCGC
  CTCTGCCTGCCACACCTGGTTGCCCTCACAGAGCAGTACACAGTGACCGGGCTGATGGAA
  GCGACCCAGATGATGGTGGACATCGATGGGGACGTCCTTGTGTTCCTGGAACTGGCTCAG
  TTCCACTGTGCGTACCAGCTGGCCGACTGGTGTCTCCACCACATCTGCACCAACTACAAC
  AACGTGTGCCGCAAGTTCCCCCGAGACATGAAGGCCATGTCCCCAGAAAACCAGGAGTAT
  TTCGAGAAGCATCGGTGGCCACCTGTGTGGTACCTGAAGGAGGAAGATCATTACCAGCGG
  GCACGGAAGGAGCGTGAGAAGGAGGACTACCTCCACCTCAAGCGGCAGCCCAAACGGCGT
  TGGCTCTTCTGGAACAGTCCATCCTCCCCGTCTTCCTCGGCAGCCTCCTCCTCATCCCCA
  TCTTCCTCCTCGGCTGTGGTCTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_015178
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015178.1, NP_055993.1
• RefSeq Size: 5101 bp
• RefSeq ORF: 2184 bp
• Locus ID: 23221
• UniProt ID: Q9BYZ6
• Cytogenetics: 8p21.3
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: The protein encoded by this gene is a small Rho GTPase and a candidate tumor suppressor. The encoded protein interacts with the cullin-3 protein, a ubiquitin E3 ligase necessary for mitotic cell division. This protein inhibits the growth and spread of some types of breast cancer. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
  Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.