CHI3L2 (NM_004000) Human Untagged Clone

CAT#: SC303417

CHI3L2 (untagged)-Human chitinase 3-like 2 (CHI3L2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHI3L2
• Synonyms: CHIL2; YKL-39; YKL39
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004000 edited
  GGCCAAGGATATGGGAGCAACCACCATGGACCAGAAGTCTCTCTGGGCAGGTGTAGTGGT
  CTTGCTGCTTCTCCAGGGAGGATCTGCCTACAAACTGGTTTGCTACTTTACCAACTGGTC
  CCAGGACCGGCAGGAACCAGGAAAATTCACCCCTGAGAATATTGACCCCTTCCTATGCTC
  TCATCTCATCTATTCATTCGCCAGCATCGAAAACAACAAGGTTATCATCAAGGACAAGAG
  TGAAGTGATGCTCTACCAGACCATCAACAGTCTCAAAACCAAGAATCCCAAACTGAAAAT
  TCTCTTGTCCATTGGAGGGTACCTGTTTGGTTCCAAAGGGTTCCACCCTATGGTGGATTC
  TTCTACATCACGCTTGGAATTCATTAACTCCATAATCCTGTTTCTGAGGAACCATAACTT
  TGATGGACTGGATGTAAGCTGGATCTACCCAGATCAGAAAGAAAACACTCATTTCACTGT
  GCTGATTCATGAGTTAGCAGAAGCCTTTCAGAAGGACTTCACAAAATCCACCAAGGAAAG
  GCTTCTCTTGACTGTGGGCGTATCTGCAGGGAGGCAAATGATTGATAACAGCTATCAAGT
  TGAGAAACTGGCAAAAGATCTGGATTTCATCAACCTCCTGTCCTTTGACTTCCATGGGTC
  TTGGGAAAAGCCCCTTATCACTGGCCACAACAGCCCTCTGAGCAAGGGGTGGCAGGACAG
  AGGGCCAAGCTCCTACTACAATGTGGAATATGCTGTGGGGTACTGGATACATAAGGGAAT
  GCCATCAGAGAAGGTGGTCATGGGCATCCCCACATATGGGCACTCCTTCACACTGGCCTC
  TGCAGAAACCACCGTGGGGGCCCCTGCCTCTGGCCCTGGAGCTGCTGGACCCATCACAGA
  GTCTTCAGGCTTCCTGGCCTATTATGAGATCTGCCAGTTCCTGAAAGGAGCCAAGATCAC
  GCGGCTCCAGGATCAGCAGGTTCCCTACGCAGTCAAGGGGAACCAGTGGGTGGGCTATGA
  TGATGTGAAGAGTATGGAGACCAAGGTTCAGTTCTTAAAGAATTTAAACCTGGGAGGAGC
  CATGATCTGGTCTATTGACATGGATGACTTCACTGGCAAATCCTGCAACCAGGGCCCTTA
  CCCTCTTGTCCAAGCAGTCAAGAGAAGCCTTGGCTCCCTGTGAAGGATTAACTTACAGAG
  AAGCAGGCAAGATGACCTTGCTGCCTGGGGCCTGCTCTCTCCCAGGAATTCTCATGTGGG
  ATTCCCCTTGCCAGGCCGGCCTTTGGATCTCTCTTCCAAGCCTTTCCTGACTTCCTCTTA
  GATCATAGATTGGACCTGGTTTTGTTTTCCTGCAGCTGTTGACTTGTTGCCCTGAAGTAC
  AATAAAAAAAATTCATTTTGCTCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004000 unedited
  NNNGGTGCAGTTCAGAACTTTGTATAACGACTCACTATAGGGCGGCCGCGACATCAAATC
  TGGTACCGGTCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGGCCAAGGATATGGG
  AGCAACCACCATGGACCAGAAGTCTCTCTGGGCAGGTGTAGTGGTCTTGCTGCTTCTCCA
  GGGAGGATCTGCCTACAAACTGGTTTGCTACTTTACCAACTGGTCCCAGGACCGGCAGGA
  ACCAGGAAAATTCACCCCTGAGAATATTGACCCCTTCCTATGCTCTCATCTCATCTATTC
  ATTCGCCAGCATCGAAAACAACAAGGTTATCATCAAGGACAAGAGTGAAGTGATGCTCTA
  CCAGACCATCAACAGTCTCAAAACCAAGAATCCCAAACTGAAAATTCTCTTGTCCATTGG
  AGGGTACCTGTTTGGTTCCAAAGGGTTCCACCCTATGGTGGATTCTTCTACATCACGCTT
  GGAATTCATTAACTCCATAATCCTGTTTCTGAGGAACCATAACTTTGATGGACTGGATGT
  AAGCTGGATCTACCCAGATCAGAAAGAAAACACTCATTTCACTGTGCTGATTCATGAGTT
  AGCAGAAGCCTTTCAGAAGGACTTCACAAAATCCACCAAGGAAAGGCTTCTCTTGACTGT
  GGGCGTATCTGCAGGGAGGCAAATGATTGATAACAGCTATCAAGTTGAGAAACTGGCAAA
  AGATCTGGATTTCATCAACCTCCTGTCCTTTGACTTCCATGGGGTCTTGGNGAAAAGCCC
  CTTATCACTGGCCACAACAGCCCTCTGAGCNAAGGGGTGGCANGACAGAGGGCCAAGCTC
  CCTACTACATGTGGAATATGCNTGTGGNGTACTGGATACATAAGGGAATGCCATCGAGNA
  AGGTGGTCATGGGCATCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004000
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. These result in the substitution of 1 aa.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004000.2, NP_003991.2
• RefSeq Size: 1486 bp
• RefSeq ORF: 1173 bp
• Locus ID: 1117
• UniProt ID: Q15782
• Cytogenetics: 1p13.2
• Gene Summary: The protein encoded by this gene is similar to bacterial chitinases but lacks chitinase activity. The encoded protein is secreted and is involved in cartilage biogenesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
  Transcript Variant: This variant (1) encodes the longest isoform (a).