PF4 (NM_002619) Human Untagged Clone

CAT#: SC303228

PF4 (untagged)-Human platelet factor 4 (PF4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PF4
• Synonyms: CXCL4; PF-4; SCYB4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002619 edited
  CCGCAGCATGAGCTCCGCAGCCGGGTTCTGCGCCTCACGCCCCGGGCTGCTGTTCCTGGG
  GTTGCTGCTCCTGCCACTTGTGGTCGCCTTCGCCAGCGCTGAAGCTGAAGAAGATGGGGA
  CCTGCAGTGCCTGTGTGTGAAGACCACCTCCCAGGTCCGTCCCAGGCACATCACCAGCCT
  GGAGGTGATCAAGGCCGGACCCCACTGCCCCACTGCCCAACTGATAGCCACGCTGAAGAA
  TGGAAGGAAAATTTGCTTGGACCTGCAAGCCCCGCTGTACAAGAAAATAATTAAGAAACT
  TTTGGAGAGTTAGCTACTAGCTGCCTACGTGTGTGCATTTGCTATA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002619 unedited
  CGTTCNAATATTTGTATACGACTCATATAGGCGGCCGCGNATTCGCCCTTAGCATGAGCT
  CCGCAGCCGGGTTCTGCGCCTCACGCCCCGGGCTGCTGTTCCTGGGGTTGCTGCTCCTGC
  CACTTGTGGTCGCCTTCGCCAGCGCTGAAGCTGAAGAAGATGGGGACCTGCAGTGCCTGT
  GTGTGAAGACCACCTCCCAGGTCCGTCCCAGGCACATCACCAGCCTGGAGGTGATCAAGG
  CCGGACCCCACTGCCCCACTGCCCAACTGATAGCCACGCTGAAGAATGGAAGGAAAATTT
  GCTTGGACCTGCAAGCCCCGCTGTACAAGAAAATAATTAAGAAACTTTTGGAGAGTTAGC
  TACTAGCTGCCTACGTGTGTGCATTTGCTATAAGGGCGAATTCAGATCTGGTACCGATAT
  CAAGCTTGTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGG
  TGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTG
  CCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTC
  TATAATATTATGGNGTGGAGGGGGGTGGGTATGGAGCAAGGGGCAAGTTGGGAAAGACAC
  CTGTAGGGCCCTGCGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCT
  CACTGCAATCTCCGNCTNCCTGGTTCAAGCGATTCTTCTGCCTCAGCCTCCCGNATTTGT
  TGGGATTCCAAGCATGCCTGACCAGGCTCAGCTAAATTTTGGTTTTTTGGGAAAGACGGG
  GTTTCACCATATTGGCCAGCCTGGTCTTCAACTCCTAATCTCAGGTGATCTACCCACCTT
  GGGCTCCCAAATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002619 unedited
  AGNAAAGCACTGGGGNAGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTA
  TGACCGCGGCCGCAATCTAGAGTCGACAAGCTTGATATCGGTACCAGATCTGAATTCGCC
  CTTATAGCAAATGCACACACGTAGGCAGCTAGTAGCTAACTCTCCAAAAGTTTCTTAATT
  ATTTTCTTGTACAGCGGGGCTTGCAGGTCCAAGCAAATTTTCCTTCCATTCTTCAGCGTG
  GCTATCAGTTGGGCAGTGGGGCAGTGGGGTCCGGCCTTGATCACCTCCAGGCTGGTGATG
  TGCCTGGGACGGACCTGGGAGGTGGTCTTCACACACAGGCACTGCAGGTCCCCATCTTCT
  TCAGCTTCAGCGCTGGCGAAGGCGACCACAAGTGGCAGGAGCAGCAACCCCAGGAACAGC
  AGCCCGGGGCGTGAGGCGCAGAACCCGGCTGCGGAGCTCATGCTAAGGGCGAATTCGCGG
  CCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTAT
  ATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGCGGGGTTATTACG
  ACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGG
  GTGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGGTGTACTGCCA
  AAACCGCATCACCATGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAA
  GTCCCGTAAAGTCATGTACTGGGCATAATGCCAGGCGGNCCATTTACCGTCATTGACGTC
  AATANGGGGCGGNACTGGNCATATGATACACT
  
• Restriction Sites: Please inquire
• ACCN: NM_002619
• Insert Size: 350 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002619.1, NP_002610.1
• RefSeq Size: 439 bp
• RefSeq ORF: 306 bp
• Locus ID: 5196
• UniProt ID: P02776
• Cytogenetics: 4q13.3
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction
• Gene Summary: This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. The protein also exhibits antimicrobial activity against Plasmodium falciparum. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.