GATA3 (NM_001002295) Human Untagged Clone

CAT#: SC300437

GATA3 (untagged)-Human GATA binding protein 3 (GATA3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GATA3
• Synonyms: HDR; HDRS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001002295 edited
  CGTCTTGATACTTTCAGAAAGAATGCATTCCCTGTAAAAAAAAAAAAAAAAAATACTGAG
  AGAGGGAGAGAGAGAGAGAAGAAGAGAGAGAGACGGAGGGAGAGCGAGACAGAGCGAGCA
  ACGCAATCTGACCGAGCAGGTCGTACGCCGCCGCCTCCTCCTCCTCTCTGCTCTTCGCTA
  CCCAGGTGACCCGAGGAGGGACTCCGCCTCCGAGCGGCTGAGGACCCCGGTGCAGAGGAG
  CCTGGCTCGCAGAATTGCAGAGTCGTCGCCCCTTTTTACAACCTGGTCCCGTTTTATTCT
  GCCATACCCAGTTTTTGGATTTTTGTCTTCCCCTTCTTCTCTTTGCTAAACGACCCCTCC
  AAGATAATTTTTAAAAAACCTTCTCCTTTGCTCACCTTTGCTTCCCAGCCTTCCCATCCC
  CCCACCGAAAGCAAATCATTCAACGACCCCCGACCCTCCGACGGCAGGAGCCCCCCGACC
  TCCCAGGCGGACCGCCCTCCCTCCCCGCGCGCGGGTTCCGGGCCCGGCGAGAGGGCGCGA
  GCACAGCCGAGGCCATGGAGGTGACGGCGGACCAGCCGCGCTGGGTGAGCCACCACCACC
  CCGCCGTGCTCAACGGGCAGCACCCGGACACGCACCACCCGGGCCTCAGCCACTCCTACA
  TGGACGCGGCGCAGTACCCGCTGCCGGAGGAGGTGGATGTGCTTTTTAACATCGACGGTC
  AAGGCAACCACGTCCCGCCCTACTACGGAAACTCGGTCAGGGCCACGGTGCAGAGGTACC
  CTCCGACCCACCACGGGAGCCAGGTGTGCCGCCCGCCTCTGCTTCATGGATCCCTACCCT
  GGCTGGACGGCGGCAAAGCCCTGGGCAGCCACCACACCGCCTCCCCCTGGAATCTCAGCC
  CCTTCTCCAAGACGTCCATCCACCACGGCTCCCCGGGGCCCCTCTCCGTCTACCCCCCGG
  CCTCGTCCTCCTCCTTGTCGGGGGGCCACGCCAGCCCGCACCTCTTCACCTTCCCGCCCA
  CCCCGCCGAAGGACGTCTCCCCGGACCCATCGCTGTCCACCCCAGGCTCGGCCGGCTCGG
  CCCGGCAGGACGAGAAAGAGTGCCTCAAGTACCAGGTGCCCCTGCCCGACAGCATGAAGC
  TGGAGTCGTCCCACTCCCGTGGCAGCATGACCGCCCTGGGTGGAGCCTCCTCGTCGACCC
  ACCACCCCATCACCACCTACCCGCCCTACGTGCCCGAGTACAGCTCCGGACTCTTCCCCC
  CCAGCAGCCTGCTGGGCGGCTCCCCCACCGGCTTCGGATGCAAGTCCAGGCCCAAGGCCC
  GGTCCAGCACAGAAGGCAGGGAGTGTGTGAACTGTGGGGCAACCTCGACCCCACTGTGGC
  GGCGAGATGGCACGGGACACTACCTGTGCAACGCCTGCGGGCTCTATCACAAAATGAACG
  GACAGAACCGGCCCCTCATTAAGCCCAAGCGAAGGCTGTCTGCAGCCAGGAGAGCAGGGA
  CGTCCTGTGCGAACTGTCAGACCACCACAACCACACTCTGGAGGAGGAATGCCAATGGGG
  ACCCTGTCTGCAATGCCTGTGGGCTCTACTACAAGCTTCACAATATTAACAGACCCCTGA
  CTATGAAGAAGGAAGGCATCCAGACCAGAAACCGAAAAATGTCTAGCAAATCCAAAAAGT
  GCAAAAAAGTGCATGACTCACTGGAGGACTTCCCCAAGAACAGCTCGTTTAACCCGGCCG
  CCCTCTCCAGACACATGTCCTCCCTGAGCCACATCTCGCCCTTCAGCCACTCCAGCCACA
  TGCTGACCACGCCCACGCCGATGCACCCGCCATCCAGCCTGTCCTTTGGACCACACCACC
  CCTCCAGCATGGTCACCGCCATGGGTTAGAGCCCTGCTCGATGCTCACAGGGCCCCCAGC
  GAGAGTCCCTGCAGTCCCTTTCGACTTGCATTTTTGCAGGAGCAGTATCATGAAGCCTAA
  ACGCGATGGATATATGTTTTTGAAGGCAGAAAGCAAAATTATGTTTGCCACTTTGCAAAG
  GAGCTCACTGTGGTGTCTGTGTTCCAACCACTGAATCTGGACCCCATCTGTGAATAAGCC
  ATTCTGACTCATATCCCCTATTTAACAGGGTCTCTAGTGCTGTGAAAAAAAAAAATGCTG
  AACATTGCATATAACTTATATTGTAAGAAATACTGTACAATGACTTTATTGCATCTGGGT
  AGCTGTAAGGCATGAAGGATGCCAAGAAGTTTAAGGAATATGGGAGAAATAGTGTGGAAA
  TTAAGAAGAAACTAGGTCTGATATTCAAATGGACAAACTGCCAGTTTTGTTTCCTTTCAC
  TGGCCACAGTTGTTTGATGCATTAAAAGAAAATAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001002295 unedited
  CGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGTCTTGATACTTTCAGAAAGAT
  GCATTCCCTGTAAAAAAAAAAAAAAAAAATACTGGAGAGAGGGGAGAGAGAGAGAGAAGA
  AGAGAGAGAGACGGAGGGAGAGCGAGACAGAGCGAGCAACGCAATCTGACCGAGCAGGTC
  GTACGCCGCCGCCTCCTCCTCCTCTCTGCTCTTCGCTACCCAGGTGACCCGAGGAGGGAC
  TCCGCCTCCGAGCGGCTGAGGACCCCGGTGCAGAGGAGCCTGGCTCGCAGAATTGCAGAG
  TCGTCGCCCCTTTTTACAACCTGGTCCCGTTTTATTCTGCCATACCCAGTTTTTGGATTT
  TTGTCTTCCCCTTCTTCTCTTTGCTAAACGACCCCTCCAAGATAATTTTTAAAAAACCTT
  CTCCTTTGCTCACCTTTGCTTCCCAGCCTTCCCATCCCCCCACCGAAAGCAAATCATTCA
  ACGACCCCCGACCCTCCGACGGCAGGAGCCCCCCGACCTCCCAGGCGGACCGCCCTCCCT
  CCCCGCGCGCGGGTTCCGGGCCCGGCGAGAGGGCGCGAGCACAGCCGAGGCCATGGAGGT
  GACGGCGGACCANCCGCGCTGGGTGAGCCACCACCACCCCGCCGTGCTCAACGGGCAGCA
  CCCGGACACGCACCACCCGGGCCTCAGCCACTCCTACATGGACGCGGCGCANTACCCGCT
  GCCGGAGGAGGTGGATGTGCTTTTTAACATCGACGGTCAAGGGCACCACGTNCCGCCCTT
  ACTACGGAACTCGGTCAGGGCCACGGTGCANAGGTACCCTCCGACCCACACGGGAGCCAN
  GTGTGCCGCCCGCCTCTGCTCATGGATCCCCTACCTGGCTGGACNGCNGCAAGCCCTGGG
  GCAGCACACACCGNCTCCCCTGGATNTNANCCTNTNCAAGAGTCATCACACGGNTCCGGG
  CCCTTTCGTTACCCGGNCTGTCTCTTTTGTGGGGCAGCACGNTTTTTTTTTTTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_001002295 unedited
  TATGTACGCGGCCGCATCTATAGTCGGTTTTTTTTTTTTTTTTTTATTTTCTTTTAATGC
  ATCAAACAACTGTGGCCAGTGAAAGGAAACAAAACTGGCAGTTTGTCCATTTGAATATCA
  GACCTAGTTTCTTCTTAATTTCCACACTATTTCTCCCATATTCCTTAAACTTCTTGGCAT
  CCTTCATGCCTTACAGCTACCCAGATGCAATAAAGTCATTGTACAGTATTTCTTACAATA
  TAAGTTATATGCAATGTTCAGCATTTTTTTTTTTCACAGCACTAGAGACCCTGTTAAATA
  GGGGATATGAGTCAGAATGGCTTATTCACAGATGGGGTCCAGATTCAGTGGTTGGAACAC
  AGACACCACAGTGAGCTCCTTTGCAAAGTGGCAAACATAATTTTGCTTTCTGCCTTCAAA
  AACATATATCCATCGCGTTTAGGCTTCATGATACTGCTCCTGCAAAAATGCAAGTCGAAA
  GGGACTGCAGGGACTCTCGCTGGGGGCCCTGTGAGCATCGAGCAGGGCTCTAACCCATGG
  CGGTGACCATGCTGGAGGGGTGGTGTGGTCCAAAGGACAGGCTGGATGGCGGGTGCATCG
  GCGTGGGCGTGGTCAACATGTGGCTGGAGTGGCTGAAAGGCGATATGTGGCTCAAGGAGG
  ACATGTGTCTGGAGAGGCCGCCGGGTTAAACGAGCTGTTCCTGGGGAAGCCTNCAGTGAG
  TCATGCACTTTTTGCACTTTTAGGATTGCTGAACATTTTCGTTCTGACTGGATGCCTTCC
  TCTTCAAGTAGGGGGCTGTAATATTGGAACCTGGAGAAAGCCCAAGCCTTTGAAAAAGGG
  CCCATTGGCTTCCTCCCCAAAGGTGGTGGGGGGGGCGAACATTCCACCAGAAGTCCTGGT
  TTCGGGTGGAAAACCCTTCTTGGGCTATAAAGGGCGGGTTGGCCTTCTTGGGATAAACCC
  CAGGCTCCAAGTGTGCCCGCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001002295
• Insert Size: 2400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002295.1, NP_001002295.1
• RefSeq Size: 3070 bp
• RefSeq ORF: 1335 bp
• Locus ID: 2625
• UniProt ID: P23771
• Cytogenetics: 10p14
• Protein Families: Adult stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors
• Gene Summary: This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).